Assessment of Wound-Healing Properties of Medicinal Plants: The Case of Phyllanthus muellerianus

Boakye, Yaw Duah; Agyare, Christian; Ayande, George P.; Titiloye, Nicholas; Asiamah, Emmanuel Akomanin; Danquah, Kwabena Owusu

doi:10.3389/fphar.2018.00945

Cited by 56 publications

(45 citation statements)

References 45 publications

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“…It has been documented that the amount of hydroxyproline in wound tissue is widely used as a measure of collagen quality (Boakye et al, 2018). To validate our histological findings, hydroxyproline contents in the lesions were assessed, and the results revealed that hydroxyproline contents in the wound tissues of SPPE-treated rats were higher.…”

Section: Discussionmentioning

confidence: 51%

Effects of Methanolic Extract Based-Gel From Saudi Pomegranate Peels With Enhanced Healing Potential on Excision Wounds in Diabetic Rats

et al. 2021

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Introduction: Current study was designed to evaluate the wound healing activity of a Saudi pomegranate peel extract on excision wound healing in experimentally induced diabetes in rats.Methodology: Animals were divided into three groups: diabetic excision wound with no treatment, diabetic excision wound with gel alone and diabetic excision wound with Saudi pomegranate peel extract in gel. Animals were monitored for clinical signs, weekly body weight, morbidity and mortality during entire study period. The efficacy parameters evaluated were percent wound contraction, Hydroxyproline content, estimation of Transforming Growth Factor ß1 (TGF-ß1), Vascular Endothelial Growth Factor (VEGF), and Epidermal Growth Factor (EGF) in wound lysates by ELISA, mRNA expression of TGF-ß1, VEGF, and EGF in wound lysates by qPCR, Estimation of nitric oxide (NO) and NO synthase (NOS) in Wound Lysates and histopathology of skin for reepithelization, neovascularization, and inflammation.Results: The Saudi pomegranate peel extract in gel (5.0 g extract per 100 g gel) showed significant wound healing activity when compared to the vehicle control [p < 0.05] following 21 days of treatment. Animals in the control and treatment groups were apparently normal through the study with no significant differences in body weights between groups. Expression of mRNA of TGFβ1, EGF and VEGF in wounds was the highest on day 14 post treatment 4.3, 3.5 and 0.9 fold higher respectively in the treatment group when compared to vehicle control, and on day 21, the values were 0.12, 0.3 and 0.83, respectively. No statistically significant differences were observed in TGF-ß1 levels in wounds on days 4, 7, 14 and 21 post treatment when compared to the vehicle control (p > 0.05). Significantly higher levels of VEGF were observed in treatment group on day 7 and 21 when compared to vehicle control (p < 0.05). Significantly higher levels of EGF were observed in treatment group on day 7 and 21 when compared to vehicle control (p < 0.05). Mean hydroxyproline levels were higher in treatment group on days 4 and 7 when compared to vehicle control. NO levels in treatment group were significantly lower on days 7, 14 and 21 when compared to vehicle control (p < 0.05). NOS activity in treatment group were significantly lower on days 4 and 7 when compared to vehicle control (p < 0.05). Histopathological changes in skin wound in the treatment group were consistent with wound healing when compared to the vehicle group.Conclusion: This study’s findings suggest that topical application of SPPE gel effectively enhanced wound healing in experimentally induced diabetic conditions.

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Section: Discussionmentioning

confidence: 51%

Effects of Methanolic Extract Based-Gel From Saudi Pomegranate Peels With Enhanced Healing Potential on Excision Wounds in Diabetic Rats

et al. 2021

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“…Like many countries in Sub-Saharan Africa, Ghana hosts a number of plants with medicinal properties [25]. However, just a handful of studies have exploited the activity of these plants against wound infected bacterial pathogens [14,18]. For instance, Boakye et al [18] determined the in vivo wound-healing activity of aqueous aerial part extract of a medicinal plant using the case of only Phyllanthus muellerianus.…”

Section: Discussionmentioning

confidence: 99%

“…However, just a handful of studies have exploited the activity of these plants against wound infected bacterial pathogens [14,18]. For instance, Boakye et al [18] determined the in vivo wound-healing activity of aqueous aerial part extract of a medicinal plant using the case of only Phyllanthus muellerianus. Agyare et al [14] also evaluated the antimicrobial and wound healing potential of two plants; Justicia flava and Lannea welwitschii.…”

Section: Discussionmentioning

confidence: 99%

“…For example, in Nigeria, Udegbunam and colleagues investigated the wound healing and antibacterial properties of Pupalia lappacea Juss [16], Crinum jagus [17] and Stephania dinklagei [17]. In Ghana, Boakye et al [18] determined the in vivo wound-healing activity of aqueous aerial part extract of a medicinal plant using the case of only Phyllanthus muellerianus. Also, Agyare and colleagues [14] evaluated the antimicrobial and wound healing potential of two plants; Justicia flava and Lannea welwitschii.…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial Activity of Selected Medicinal Plants from a Sub-Saharan African Country against Bacterial Pathogens from Post-Operative Wound Infections

et al. 2021

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Background: Globally, the application of medicinal plants in the management of acute and chronic wounds can be considered a common occurrence in most traditional medicine practices. In view of this, many plants in the tropical and subtropical regions have been screened for their wound-healing activities. Consequently, plants having antimicrobial activity against multidrug-resistant (MD-R) pathogens can be considered great assets. Therefore, this study evaluated ethanolic and aqueous extracts of five medicinal plants (Psidium guajava, Myrianthus arboreus, Alchornea cordifolia, Momordica charantia, and Justicia flava) for their antimicrobial activities against MD-R bacterial pathogens isolated from post-operative wounds; Methods: This involved the aqueous and ethanolic extraction of the selected medicinal plants. Preliminary phytochemical constituents of the plants were examined. The agar well diffusion method was then used to determine the antibacterial activity of the leaves against reference strains (Escherichia coli ATCC 25922, Salmonella typhi ATCC 19430, Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus ATCC 25923, and a Methicillin-Resistant Staphylococcus aureus strain) as well as the MDR clinical isolates (E. coli, P. aeruginosa, S. aureus and CoNS) from the wounds; Results: The preliminary phytochemical analysis of the leaves showed the presence of saponins, phenolics, and reducing sugars in almost all the plants tested. All plant extracts were observed to have some antimicrobial activity against at least one reference strain. For the clinical MDR isolates, A. cordifolia from this study showed highest inhibition to growth of all bacteria used. Activity of J. flava against S. aureus was highest as compared to that of E.coli and P. aeruginosa. Similar observation was made for M. arboreus, P. guajava and M. charantia where the highest activity was observed against S. aureus; Conclusion: This study has mainly shown that P. guajava, M. arboreus, A. cordifolia, M. charantia, and J. flava exhibits antimicrobial activities against MD-R bacterial pathogens isolated from post-operative wounds. Also, these plants has bioactive phytochemical compounds with potential medicinal values for the treatment of numerous infections. Therefore, these plants may be helpful in the management of acute and chronic wounds, especially in traditional medicine practices.

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“…A similar test was done using 1% dimethyl sulfoxide (DMSO) solution (used as a negative control for this test), and the absorbance ( Ad ) was measured [ 19 , 20 ]. The IC 50 for CFHE was estimated.…”

Section: Methodsmentioning

confidence: 99%

Wound Management Property of a Hydroethanolic Leaf Extract of Cnestis ferruginea DC

Jibira

Koffuor

Atsu-Nunyawu

et al. 2021

Advances in Pharmacological and Pharmaceutical Sciences

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Context. Nature assumes imperative part in managing both acute and chronic ailments. Cnestis ferruginea is one of the sources of healing prowlers in traditional medicine practice for the treatment of wounds. Nonetheless, there are no scientific reports archived so far on the wound management activity of this plant. Objective. To establish the wound management property of a hydroethanolic Cnestis ferruginea leaf extract (CFHE). Materials and Methods. The wound area was measured after excision at the dorsal part of the Albino rats, and after treatment with 5–15% w / w CFHE ointments for 14 days. Absorbances of platelet-rich plasma treated with 0.8–100 mg/mL CFHE and an aggregating agent were spectrophotometrically determined in an in vitro platelet aggregation test. Wound tissue histopathology of CFHE ointment-treated animals revealed angiogenesis, reepithelialization, deposition of collagen, and granular tissue formation in wound tissues. Reduction in thigh oedema and pain threshold, in 7-day-old chicks, were assessed by carrageenan-induced oedema and Randall-Sellito pressure models, respectively. By the Agar diffusion method, bacterial growth inhibition by a 15% w / w CFHE ointment was investigated on Salmonella typhi, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus. Aureus, and Streptococcus pyogenes. Results. All concentrations of CFHE ointment significantly reduced ( p < 0.0001 ) wound area by 29–41% posttreatment. CFHE (1.6–100 mg/ml) promoted platelet aggregation ( p ≤ 0.0001 ) by 37–67% (IC50: 3.1–6.2 mg/ml). There were improved wound tissue reepithelization, fibroblast proliferation, angiogenesis, and collagen deposition with 15% CFHE ointment treatment. CFHE ointment significantly ( p ≤ 0.0001 ) and dose-dependently reduced thigh oedema and showed a significant ( p ≤ 0.05 ) analgesic effect. In vitro, 15% CFHE ointment caused >100% growth inhibition of selected bacteria. Conclusion. The hydroethanolic leaf extract of Cnestis ferruginea possesses wound healing, platelet aggregation, anti-inflammatory, analgesic, and antimicrobial properties and, hence, could be effective in the management of open and some closed wounds.

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Assessment of Wound-Healing Properties of Medicinal Plants: The Case of Phyllanthus muellerianus

Cited by 56 publications

References 45 publications

Effects of Methanolic Extract Based-Gel From Saudi Pomegranate Peels With Enhanced Healing Potential on Excision Wounds in Diabetic Rats

Effects of Methanolic Extract Based-Gel From Saudi Pomegranate Peels With Enhanced Healing Potential on Excision Wounds in Diabetic Rats

Antimicrobial Activity of Selected Medicinal Plants from a Sub-Saharan African Country against Bacterial Pathogens from Post-Operative Wound Infections

Wound Management Property of a Hydroethanolic Leaf Extract of Cnestis ferruginea DC

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