Assessment of variability in secondary metabolites and expected response to genotype selection in fenugreek (Trigonella spp.)

Riasat, Mehrnaz; Heidari, Bahram; Pakniyat, Hassan; Jafari, Ali

doi:10.1016/j.indcrop.2018.06.068

Cited by 18 publications

(7 citation statements)

References 40 publications

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“…Accumulation of secondary metabolites is not only influenced by environmental factors (e.g., temperature, light, the supply of water and minerals) but also genotypes (e.g., variety, strain and cultivar) [ 21 , 22 , 23 ]. Previous studies have demonstrated that there is a significant difference of secondary metabolites among the three Angelica species: A. sinensis (Oliver) Diels, A. dahurica (Fisch.…”

Section: Discussionmentioning

confidence: 99%

Integrated Metabolomic and Transcriptomic Analysis Reveals Differential Mechanism of Flavonoid Biosynthesis in Two Cultivars of Angelica sinensis

Zhu

Zhang

et al. 2022

Molecules

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Angelica sinensis is a traditional Chinese medicinal plant that has been primarily used as a blood tonic. It largely relies on its bioactive metabolites, which include ferulic acid, volatile oils, polysaccharides and flavonoids. In order to improve the yield and quality of A. sinensis, the two cultivars Mingui 1 (M1), with a purple stem, and Mingui 2 (M2), with a green stem, have been selected in the field. Although a higher root yield and ferulic acid content in M1 than M2 has been observed, the differences of flavonoid biosynthesis and stem-color formation are still limited. In this study, the contents of flavonoids and anthocyanins were determined by spectrophotometer, the differences of flavonoids and transcripts in M1 and M2 were conducted by metabolomic and transcriptomic analysis, and the expression level of candidate genes was validated by qRT-PCR. The results showed that the contents of flavonoids and anthocyanins were 1.5- and 2.6-fold greater in M1 than M2, respectively. A total of 26 differentially accumulated flavonoids (DAFs) with 19 up-regulated (UR) and seven down-regulated (DR) were obtained from the 131 identified flavonoids (e.g., flavonols, flavonoid, isoflavones, and anthocyanins) in M1 vs. M2. A total 2210 differentially expressed genes (DEGs) were obtained from the 34,528 full-length isoforms in M1 vs. M2, and 29 DEGs with 24 UR and 5 DR were identified to be involved in flavonoid biosynthesis, with 25 genes (e.g., CHS1, CHI3, F3H, DFR, ANS, CYPs and UGTs) mapped on the flavonoid biosynthetic pathway and four genes (e.g., RL1, RL6, MYB90 and MYB114) belonging to transcription factors. The differential accumulation level of flavonoids is coherent with the expression level of candidate genes. Finally, the network of DAFs regulated by DEGs was proposed. These findings will provide references for flavonoid production and cultivars selection of A. sinensis.

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Section: Discussionmentioning

confidence: 99%

Integrated Metabolomic and Transcriptomic Analysis Reveals Differential Mechanism of Flavonoid Biosynthesis in Two Cultivars of Angelica sinensis

Zhu

Zhang

et al. 2022

Molecules

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“…Fenugreek (Trigonella foenum-graecum L.) is a plant used for the culinary and medicinal uses [14]. Fenugreek seeds and fenugreek leaves are the main parts that consumed in the diet, and fenugreek plant have been used as medicines to treat some chronical diseases such as diabetes and high blood pressure [15,16]. Even though having a bitter taste of the fenugreek seed extracts, it can be possible to evaluate them in some food formulations.…”

Section: Introductionmentioning

confidence: 99%

Improving the quality attributes of mayonnaise enriched with phenolic extracts of fenugreek seeds and leaves during storage

Turker

Isleroglu

2022

Preprint

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In this study, mayonnaise was enriched with phenolic extracts of fenugreek seeds (FSE) and fenugreek leaves (FLE), and quality attributes of the enriched mayonnaises were investigated during storage. FSE and FLE were added to mayonnaises at three different levels (0.05, 0.10 and 0.20%) and the samples were stored at 4°C for 12 weeks, and at 25°C for 6 weeks. Antioxidant activity, peroxide value, titratable acidity, color change, microbial and sensorial analysis were carried out. As a result, enrichment of mayonnaise with FSE and FLE improved its quality properties during storage. FSE and FLE added samples’ antioxidant activities were preserved ~ 87 and ~ 47% at 4°C, 81% and ~ 27% at 25°C, respectively. In addition, the increase in peroxide values of enriched mayonnaise with the extracts during storage was less than that of synthetic antioxidant (EDTA) added samples. The highest total color change (ΔE) was observed for FLE added samples for all the addition levels. Addition of FLE and FSE at the highest level (0.20%) was prevented the total aerobic mesophilic bacteria growth as 2.1 and 3.2 logs, and the total yeast/mold growth as 1.0 and 1.3 logs at 25°C, respectively. Sensory attributes (color, odor, taste, texture, overall acceptance) of the FSE and FLE added mayonnaises had higher scores than the control sample at the end of the storage.

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“…Fenugreek belongs to the family of Leguminosae and its seeds are the main parts consumed in the human diet. Fenugreek is used for treating different kinds of diseases such as diabetes, fever, high blood pressure, and migraines [2]. The seed powder of fenugreek is also used as spice to flavor foods for consumption [3].…”

Section: Introductionmentioning

confidence: 99%

“…These seeds have a bitter taste and very aromatic flavor, and they have carminative, antibacterial and antioxidant properties. Fenugreek seeds have remarkable bioactive compounds, namely phenolic compounds, flavonoids and saponins, which make these seeds rewarding in research [2].…”

Section: Introductionmentioning

confidence: 99%

“…Fenugreek seeds are also rich in flavonoids. The flavonoid level of fenugreek seeds can be more than 100 mg per g of dry seed [2,8]. Gyawali and Ibrahim [9] reported that a wide variety of phenolic compounds and flavonoids which were responsible for the scavenging activity of hydroxyl radicals and antimicrobial activity existed in fenugreek seeds.…”

Section: Introductionmentioning

confidence: 99%

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Enhanced recovery of bioactive compounds from Trigonella-foenum graecum seeds by ultrasonic-assisted extraction

2021

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In this study, bioactive rich extracts were obtained from Trigonella-foenum graecum (fenugreek) seeds that are considered to have a rich content of bioactive materials using the ultrasonic-assisted extraction (UAE) method and the recovery of bioactive materials was enhanced by UAE when compared with classical maceration technique (CE). The process conditions ensuring the highest total phenolic (TPC), total flavonoid (TFL) and total saponin (TSC) compounds and antioxidant activity (AA) for both UAE and CE were determined by response surface methodology. Firstly, for CE, the effect of distilled water and ethanol concentration and sample:solvent ratio on AA was investigated. After determination of the optimum solvent mixture ratio in CE (60.938% distilled water, 39.062% ethanol), it was kept constant and effects of the sample:solvent ratio, ultrasonic amplitude and extraction time on the recovery of bioactive materials were investigated for UAE process. UAE enhanced the recovery of TPC, TFL, TSC and AA as 28, 25, 26 and 21%, respectively when compared with CE. This enhancement by UAE was ensured at the half of the extraction time of CE. The optimum process conditions for UAE providing the highest AA values as follows: 13.26 g/L sample:solvent ratio, 54.72% ultrasonic amplitude and 59.71 minutes of extraction time.

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Assessment of variability in secondary metabolites and expected response to genotype selection in fenugreek (Trigonella spp.)

Cited by 18 publications

References 40 publications

Integrated Metabolomic and Transcriptomic Analysis Reveals Differential Mechanism of Flavonoid Biosynthesis in Two Cultivars of Angelica sinensis

Integrated Metabolomic and Transcriptomic Analysis Reveals Differential Mechanism of Flavonoid Biosynthesis in Two Cultivars of Angelica sinensis

Improving the quality attributes of mayonnaise enriched with phenolic extracts of fenugreek seeds and leaves during storage

Enhanced recovery of bioactive compounds from Trigonella-foenum graecum seeds by ultrasonic-assisted extraction

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