Assessment of variability in acquired thermotolerance: Potential option to study genotypic response and the relevance of stress genes

Senthil‐Kumar, Muthappa; Kumar, Ganesh; Srikanthbabu, Venkatachalayya; Udayakumar, M.

doi:10.1016/j.jplph.2006.09.009

Cited by 76 publications

(62 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Further alteration in membrane characteristics, chlorophyll degradation, cell viability and free radical production on exposure to high or low temperature substantiate the earlier reports (Siripornadulsil et al 2002;Senthil-Kumar et al 2003, 2007) that responses to these can be elucidated in Chlamydomonas. Chlamydomonas has also been shown to respond UV stress both transcriptionally and post-transcriptionally .…”

Section: Discussionsupporting

confidence: 87%

“…However, in all these studies, cells were directly exposed to severe stress levels and growth response was studied. Many studies have shown the ability of plants and other organisms to tolerate otherwise lethal stress upon prior exposure to sub lethal stress often referred to as induction stress (reviewed in Bartels and Sunkar 2005;Senthil-Kumar et al 2007). During induction stress, many stress-responsive genes are expressed which, in-turn trigger several physiological and biochemical processes relevant for stress tolerance.…”

Section: Discussionmentioning

confidence: 99%

“…There are evidences to show that several transgenics expressing stress responsive genes did not show elevated levels of tolerance over wild type following acclimation (Prandl et al 1998; also reviewed in Senthil- Kumar et al 2007). These studies conWrm that the acclimation response of the transgene will alone provide clues about its relevance.…”

Section: Genetic Transformation and Expression Of Coda Gene In Chlamymentioning

confidence: 99%

See 2 more Smart Citations

Chlamydomonas reinhardtii, a model system for functional validation of abiotic stress responsive genes

Hema

Senthil‐Kumar

Shivakumar

et al. 2007

Planta

View full text Add to dashboard Cite

Stress tolerance is a multigenic character and there are many stress responsive genes, which are stress specific. Although many of these have been cloned, their functional significance remains fragmentary. Hence it is important to identify the relevant stress genes involved in altering the metabolism for adaptation. Overexpression is one of the several approaches and Chlamydomonas is a suitable system to study the functional relevance of stress genes. Stress responses can only be assessed on prior exposure to sublethal induction stress. In this study the acclimation response of Chlamydomonas was assessed for different abiotic stresses using physiological screens like chlorophyll stability, membrane damage, cell viability, accumulation of free radicals, survival and recovery growth. We demonstrate that Chlamydomonas responds to diverse stresses and is a potential system to study the relevance of stress genes. The relevance of choline oxidase A (codA), a key enzyme in glycinebetaine biosynthesis, was examined by developing transformants expressing codA gene from Arthrobacter globiformis. Southern positive transformants showed enhanced accumulation of glycinebetaine. The transformants also showed enhanced growth under salinity, high light coupled with methylviologen-induced oxidative stress, high temperature and cold stress. However the transgenics were not tolerant to PEG-mediated simulated osmotic stress, LiCl, menadione and UV stress. Increased cell survival and decreased chlorophyll degradation in transformants under acclimated conditions further confirmed the relevance of codA in imparting stress tolerance. Our results indicated that the relevance of stress responsive genes can be efficiently validated for diverse abiotic stresses using Chlamydomonas system.

show abstract

Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Genetic Transformation and Expression Of Coda Gene In Chlamymentioning

confidence: 99%

See 1 more Smart Citation

Chlamydomonas reinhardtii, a model system for functional validation of abiotic stress responsive genes

Hema

Senthil‐Kumar

Shivakumar

et al. 2007

Planta

View full text Add to dashboard Cite

show abstract

“…Temperature-stress-acclimated plants are known to activate a wide range of stress-responsive pathways, and this can contribute to tolerance upon exposure to severe stress. 22 Moreover, this result demonstrates that the high temperature tolerance of mutant and transgenic plants can be best assessed after exposure to acclimation stress. Consistently, earlier studies have shown that acclimation-based high temperature stress imposition was useful to best study the relevance of candidate genes that impart thermotolerance.…”

Section: Introductionmentioning

confidence: 81%

“…Consistently, earlier studies have shown that acclimation-based high temperature stress imposition was useful to best study the relevance of candidate genes that impart thermotolerance. 22,23 Further, the survival frequency was significantly greater in AtCYP710A1 gene overexpressor plants when compared with wild-type and mutant plants. In order to understand the relevance of this gene in maintaining plasma membrane stability under high temperature stress, we assessed the extent of membrane leakage.…”

Section: Introductionmentioning

confidence: 96%

AtCYP710A1gene-mediated stigmasterol production plays a role in imparting temperature stress tolerance inArabidopsis thaliana

Senthil‐Kumar

Wang

Mysore

2013

Plant Signaling & Behavior

Self Cite

View full text Add to dashboard Cite

Stigmasterol and sitosterol, important sterols present in plants, are known to influence permeability and fluidity characteristics of the plasma membrane and other organellar membranes. We had previously demonstrated that the Arabidopsis Atcyp710A1 gene, which catalyzes conversion of sitosterol into stigmasterol, plays a role in plasma membrane permeability, thus influencing leakage of cellular nutrients and ions into apoplast. In this study, we investigated the role of this gene in imparting various abiotic stress tolerances in Arabidopsis. By analyzing Atcyp710a1 mutant and AtCYP710A1 overexpressor lines, we found that the AtCYP710A1 gene plays a role in imparting low and high temperature tolerance.

show abstract

Cotton: Genetic Improvement for Drought Stress Tolerance – Current Status and Research Needs

abou-kheir

Sheshshayee

Prasad

et al. 2012

Improving Crop Resistance to Abiotic Stress

View full text Add to dashboard Cite

Assessment of variability in acquired thermotolerance: Potential option to study genotypic response and the relevance of stress genes

Cited by 76 publications

References 65 publications

Chlamydomonas reinhardtii, a model system for functional validation of abiotic stress responsive genes

Chlamydomonas reinhardtii, a model system for functional validation of abiotic stress responsive genes

AtCYP710A1gene-mediated stigmasterol production plays a role in imparting temperature stress tolerance inArabidopsis thaliana

Cotton: Genetic Improvement for Drought Stress Tolerance – Current Status and Research Needs

Contact Info

Product

Resources

About