Assessment of vaginal and endometrial microbiota by real‐time <scp>PCR</scp> in women with unexplained infertility

Sezer, Özlem; Çalışkan, Canan Soyer; Çelik, Samettin; Kılıç, Süleyman; Kuruoğlu, Tuba; Ustun, Goksenin Unluguzel; Yurtçu, Nazan

doi:10.1111/jog.15060

Cited by 11 publications

(6 citation statements)

References 24 publications

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“…No statistically significant differences were observed between corresponding fresh and frozen samples, allowing the conclusion that samples can be frozen prior to processing. Previous studies reported samples for vaginal microbiome analysis have been processed immediately after collection [ 14 ], but this obviously causes challenges in the research and clinical setting. The ability to freeze samples before processing allows flexibility in sample collection, batch processing and thus, will result in cost savings.…”

Section: Discussionmentioning

confidence: 99%

“…The development of new technology has enabled rapid analysis of the vaginal microbiome with ease using quantitative real-time polymerase chain reaction (qPCR) [ 14 ]. The vaginal microbiome is not routinely assessed but in certain research settings and private scenarios, women can access the test, which is usually undertaken through a vaginal swab [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

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Menstrual Tampons Are Reliable and Acceptable Tools to Self-Collect Vaginal Microbiome Samples

Turner,

Drury,

Hapangama

et al. 2023

IJMS

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Many women report embarrassment as the cause for their avoidance of routine gynaecological screening appointments. Methods of self-collection of bio samples would perhaps encourage women to participate in routine screening programs. The vaginal microbiome plays a key role in women’s health and reproductive function. Microbial disturbances can result in the loss of lactobacillus dominance, also known as dysbiosis, associated with an increased risk of contracting sexually transmitted infections (STIs), pregnancy complications and infertility. Our primary aim was to determine if vaginal microbiome screening results are comparable between two methods for self-collected sample acquisition: tampons and lower vaginal swabs (LVSs). Secondary aims included the assessment of the effect of pre-analytic storage on the data (to streamline processing), the prevalence of dysbiosis and the acceptability of the tampons to the participants. Statistical analysis revealed no significant difference in the microbiome data, from tampons versus LVSs or fresh versus frozen samples. The prevalence of dysbiosis in this population of healthy volunteers was 42.9%. The questionnaire data revealed that 52.4% of volunteers use tampons every period, and the majority of volunteers rated the tampons as 5 on a 1–5 Likert scale regarding their perceived comfort using tampons. All (100%) of volunteers were happy to provide a tampon as a sample for testing. The findings from this study show that tampons and LVSs were comparable when analysing the vaginal microbiome, with potential superiority of the tampon with regard to patient acceptability. Self-collection of vaginal secretions for gynaecological screening using tampons warrants further research as this could change the screening landscape, ensuring wider participation and increasing efficacy.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Menstrual Tampons Are Reliable and Acceptable Tools to Self-Collect Vaginal Microbiome Samples

Turner,

Drury,

Hapangama

et al. 2023

IJMS

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“…(Babu et al ., 2017), Ureaplasma spp . ( Wee et al ., 2018 ), Chlamydia trachomatis ( Graspeuntner et al ., 2018 ), Mycoplasma hominis ( Sezer et al ., 2022 ).…”

Section: Resultsmentioning

confidence: 99%

Vaginal microbioma and the presence of Lactobacillus spp. as interferences in female fertility: A review system

Souza

Monteiro

Moura

et al. 2023

JBRA

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The vaginal microbiome is dominated by Lactobacillus spp. and the depletion of these microorganisms have been associated with adverse conditions that can affect women’s health. Disturbance of the vaginal niche with a non-lactobacillary microbiota is associated with susceptibility to some diseases, such as obstetric alterations and infertility, resulting in failure in natural pregnancies and increased demand for assisted reproduction treatments. The present study sought to understand the influence of Lactobacillus spp. and fertility female. A systematic search was performed in the following databases: PubMed, MEDLINE, SciELO and LILACS, using the keywords: “Microbiome”; “ Lactobacillus ” and “Female Infertility”, published in the last five years. The search resulted in 92 articles; however, 38 articles were excluded due to duplicity, 23 articles were excluded in the selection title/abstract, leaving 31 articles for full reading. In the end, 18 articles were analyzed. The studies encompassed a total of 2,011 women, using 27 types of samples to verify the composition of the microbiome. The eighteen articles that reported the microbiome of fertile women were constituted by a dominance of Lactobacillus spp. who joined to positive predictive outcomes in reproduction, while infertile women showed a dysbiotic profile. Therefore, analyzing bacterial patterns would allow a personalized diagnosis, which could favor personalized therapy for prevention and treatment of certain diseases.

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“…A study found that infertile women show characteristic variations in vaginal microbiome, such as the increased abundance of Atopobium, Aerococcus, and Bifidobacterium and decreased abundance of Lactobacillus and Leuconostoc (Zhao et al, 2020). In the unexplained infertility patients, the colonization rate of Lactobacillus in vaginal samples was lower, and the mean proportion of Lactobacillus/total bacterial was significant reduction (Sezer et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Urogenital tract and rectal microbiota composition and its influence on reproductive outcomes in infertile patients

Dong

Zhong²,

Zhang³

et al. 2023

Front. Microbiol.

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IntroductionMicrobiota in the human body are closely related to human diseases. Female urogenital tract and rectal microbes have been considered as important factors affecting female pregnancy, but the mechanism is unknown.MethodsCervical, vaginal, urethral, and rectal swabs were collected from 22 infertile patients and 10 controls, and follicular fluid was extracted from 22 infertile patients. The microbial composition of different sampling sites of infertile patients was examined. By comparing the microbial composition difference between infertile patients and controls and combining bioinformatics methods to analyze the potential impact of the female urogenital tract (cervical, vaginal and urethral) and rectal microbial diversity on female infertility and pregnancy outcomes.ResultsLactobacillus predominated in the female urogenital tract, but its abundance decreased in infertile patients, whereas the abundance of Gardnerella and Atopobium increased. The microbial changes in the urethra had the same trend as that in the vagina. Compared with healthy controls, the cervical and rectal microbial diversity of infertile patients were significantly increased and decreased, respectively. There might be interactions between microbes in different parts of female. Geobacillus thermogeniticans was enriched in the urogenital tract and rectum of infertile patients, and has a good predictive effect on infertility. Compared with infertile patients, L. johnsonii was enriched in the vagina, urethra, and intestine of the control group. L. acidophilus in follicular fluid might be associated with Non-pregnancy.ConclusionThis study found that the microbial composition of infertile patients was changed compared with that of healthy people. The translocation of Lactobacillus between the rectum and urogenital tract might play a protective barrier role. The changes of Lactobacillus and Geobacillus might be related to female infertility or pregnancy outcome. The study provided a theoretical basis for the future treatment of female infertility from the perspective of microorganisms by detecting the microbial changes associated with female infertility.

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Assessment of vaginal and endometrial microbiota by real‐time PCR in women with unexplained infertility

Cited by 11 publications

References 24 publications

Menstrual Tampons Are Reliable and Acceptable Tools to Self-Collect Vaginal Microbiome Samples

Menstrual Tampons Are Reliable and Acceptable Tools to Self-Collect Vaginal Microbiome Samples

Vaginal microbioma and the presence of Lactobacillus spp. as interferences in female fertility: A review system

Urogenital tract and rectal microbiota composition and its influence on reproductive outcomes in infertile patients

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