Assessment of tissue heating under tunable near-infrared radiation

Bixler, Joel N.; Hokr, Brett H.; Denton, Michael L.; Noojin, Gary D.; Shingledecker, Aurora D.; Beier, Hope T.; Thomas, Robert J.; Rockwell, Benjamin A.; Yakovlev, Vladislav V.

doi:10.1117/1.jbo.19.7.070501

Cited by 59 publications

(16 citation statements)

References 15 publications

(14 reference statements)

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“…In these experiments, the linewidth of the Brillouin peaks was difficult to determine due to a limited signal‐to‐noise ratio and was not analyzed. For future studies, in order to improve the signal quality and avoid possible thermal damage, we can take advantage of the so‐called “biological window” by selecting the NIR pump source (e.g., 780 nm, ) and increase the pump power to compensate for a lower Brillouin scattering cross‐section at longer wavelengths. Recent studies have shown the feasibility of surface enhanced Brillouin scattering using engineered metallic nanostructures .…”

Section: Resultsmentioning

confidence: 99%

Subcellular measurements of mechanical and chemical properties using dual Raman-Brillouin microspectroscopy

et al. 2015

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Brillouin microspectroscopy is a powerful technique for noninvasive optical imaging. In particular, Brillouin microspectroscopy uniquely allows assessing a sample's mechanical properties with microscopic spatial resolution. Recent advances in background-free Brillouin microspectroscopy make it possible to image scattering samples without substantial degradation of the data quality. However, measurements at the cellular- and subcellular-level have never been performed to date due to the limited signal strength. In this report, by adopting our recently optimized VIPA-based Brillouin spectrometer, we probed the microscopic viscoelasticity of individual red blood cells. These measurements were supplemented by chemically specific measurements using Raman microspectroscopy.

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Section: Resultsmentioning

confidence: 99%

Subcellular measurements of mechanical and chemical properties using dual Raman-Brillouin microspectroscopy

et al. 2015

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“…Especially in pigment-rich tissue, such as skin or retina, single-photon absorption and heating are the dominant sources of photo-induced damage. This can result, for example, in cavitation and morphological changes [55,56]. Linear damage is reduced by using shorter laser pulses.…”

Section: Two-photon Microscopymentioning

confidence: 99%

Phototoxicity in live fluorescence microscopy, and how to avoid it

et al. 2017

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Phototoxicity frequently occurs during live fluorescence microscopy, and its consequences are often underestimated. Damage to cellular macromolecules upon excitation light illumination can impair sample physiology, and even lead to sample death. In this review, we explain how phototoxicity influences live samples, and we highlight that, besides the obvious effects of phototoxicity, there are often subtler consequences of illumination that are imperceptible when only the morphology of samples is examined. Such less apparent manifestations of phototoxicity are equally problematic, and can change the conclusions drawn from an experiment. Thus, limiting phototoxicity is a prerequisite for obtaining reproducible quantitative data on biological processes. We present strategies to reduce phototoxicity, e.g. limiting the illumination to the focal plane and suggest controls for phototoxicity effects. Overall, we argue that phototoxicity needs increased attention from researchers when designing experiments, and when evaluating research findings.

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“…The sample was translated from exposure to exposure to prevent artifacts due to residual heating from prior shots. The optical system used here (aside from the source) and exposure parameters are detailed in (9). Figure 1 shows the experimentally measured thermal response of the tissue.…”

Section: Methods and Discussionmentioning

confidence: 99%