Assessment of time‐dependent white blood cells degeneration induced by blood storage on automated parameters and morphology examination

Naoum, Flávio Augusto; Martin, Fernando Henrique de Oliveira; Valejo, Mariana Ramirez; Oliveira, Maria Gabriela de Lucca

doi:10.1111/ijlh.13234

Cited by 3 publications

(4 citation statements)

References 10 publications

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“…Consistent with previous reports in other species, 5,20 a significant increase in neutrophil nuclear area indicative of nuclear swelling was found as canine blood samples aged. This occurred irrespective of storage temperature or inflammatory leukogram status.…”

Section: Discussionsupporting

confidence: 92%

“…Previous studies have shown that cellular deterioration begins within the first 4 h of sample collection, 5,8 and moderate morphologic and hematologic parameter changes are often seen within 48 h. 4,5,7,20 In the present study, a 72-h time point was also included in view of variable transit times of samples received by diagnostic laboratories. This aimed to include the typical sample transit time, as well as accounting for potential delays.…”

Section: Discussionmentioning

confidence: 99%

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Impact on canine neutrophil preservation with the addition of bovine serum albumin to K₃‐EDTA whole blood samples

Reinhart

Gilroy

Clancey

et al. 2023

Veterinary Clinical Pathol

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Blood smear evaluation is an important component of a complete blood count (CBC). Blood smear preparation and CBC data attainment are best performed on freshly collected potassium ethylene diamine tetra-acetic acid (K 3 -EDTA) anticoagulated whole blood samples. This is because delays may cause age-related deterioration and morphologic changes in erythrocytes, leukocytes, and platelets. [1][2][3][4][5][6] Cellular deterioration in aged samples negatively impacts hematologic parameters, including hematocrit, mean corpuscular

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Impact on canine neutrophil preservation with the addition of bovine serum albumin to K₃‐EDTA whole blood samples

Reinhart

Gilroy

Clancey

et al. 2023

Veterinary Clinical Pathol

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show abstract

“…Comprehensive cell population data (CPD) for subpopulations of WBC are possible with the automatic blood cell analyzer DxH800 (Coulter Inc), and this has been applied, for instance, to study WBC changes due to acute bacterial infections, G‐CSF administration, presence of CD34‐positive cells in peripheral blood, degeneration induced by storage and, recently, for COVID‐19 diagnostic workup 4‐8 …”

Section: Introductionmentioning

confidence: 99%

“…3 Comprehensive cell population data (CPD) for subpopulations of WBC are possible with the automatic blood cell analyzer DxH800 (Coulter Inc), and this has been applied, for instance, to study WBC changes due to acute bacterial infections, G-CSF administration, presence of CD34-positive cells in peripheral blood, degeneration induced by storage and, recently, for COVID-19 diagnostic workup. [4][5][6][7][8] The aim of this study was to analyze COVID-19-related abnormalities of WBC and CPD at disease onset, and identify parameters capable of discriminating between severe and nonsevere forms, as well as those with high or low risk of mortality.…”

mentioning

confidence: 99%

Diagnostic and prognostic utility of WBC counts and cell population data in patients with COVID‐19

Naoum

Ruiz²,

Martin³

et al. 2020

Int J Lab Hematology

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Introduction Early diagnosis and identification of potential critical cases for timely treatment are crucial for COVID‐19 patients. The aim of this study was to analyze the diagnostic and prognostic implications of WBC and cell population data (CPD) abnormalities related to COVID‐19 at disease onset. Methods Baseline WBC counts and CPD data were analyzed in one hundred COVID‐19 patients presenting to emergency department and subsequently discharged (n = 49), admitted (n = 51) or deceased (n = 22), and in 47 healthy subjects. Results Lymphopenia and eosinopenia were observed in all COVID‐19 patients, with more intensity in the admitted and deceased groups, that also presented increased WBC and neutrophil counts. On CPD analysis, COVID‐19 was associated with increased volume of neutrophils, lymphocytes, and monocytes, whereas conductivity was decreased for neutrophils and increased for lymphocytes. The ROC curve analysis showed good performance for lymphocyte counts in predicting COVID‐19 diagnosis (AUC = 0.858), for neutrophil counts in predicting admission for COVID‐19 (AUC = 0.744) and for monocytes volume in predicting COVID‐19 diagnosis (AUC = 0.837). Conclusion WBC counts and CPD parameters at disease onset in COVID‐19 patients can improve diagnostic characterization and aid in the discrimination between severe and nonsevere presentations.

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Single Cell Transcriptome Analysis of Peripheral Blood Mononuclear Cells in Freshly Isolated versus Stored Blood Samples

Kao

Garifallou

et al. 2023

Genes

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Background: Peripheral blood mononuclear cells (PBMCs) are widely used as a model in the study of different human diseases. There is often a time delay from blood collection to PBMC isolation during the sampling process, which can result in an experimental bias, particularly when performing single cell RNA-seq (scRNAseq) studies. Methods: This study examined the impact of different time periods from blood draw to PBMC isolation on the subsequent transcriptome profiling of different cell types in PBMCs by scRNAseq using the 10X Chromium Single Cell Gene Expression assay. Results: Examining the five major cell types constituting the PBMC cell population, i.e., CD4+ T cells, CD8+ T cells, NK cells, monocytes, and B cells, both common changes and cell-type-specific changes were observed in the single cell transcriptome profiling over time. In particular, the upregulation of genes regulated by NF-kB in response to TNF was observed in all five cell types. Significant changes in key genes involved in AP-1 signaling were also observed. RBC contamination was a major issue in stored blood, whereas RBC adherence had no direct impact on the cell transcriptome. Conclusions: Significant transcriptome changes were observed across different PBMC cell types as a factor of time from blood draw to PBMC isolation and as a consequence of blood storage. This should be kept in mind when interpreting experimental results.

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Assessment of time‐dependent white blood cells degeneration induced by blood storage on automated parameters and morphology examination

Cited by 3 publications

References 10 publications

Impact on canine neutrophil preservation with the addition of bovine serum albumin to K₃‐EDTA whole blood samples

Impact on canine neutrophil preservation with the addition of bovine serum albumin to K₃‐EDTA whole blood samples

Diagnostic and prognostic utility of WBC counts and cell population data in patients with COVID‐19

Single Cell Transcriptome Analysis of Peripheral Blood Mononuclear Cells in Freshly Isolated versus Stored Blood Samples

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Assessment of time‐dependent white blood cells degeneration induced by blood storage on automated parameters and morphology examination

Cited by 3 publications

References 10 publications

Impact on canine neutrophil preservation with the addition of bovine serum albumin to K3‐EDTA whole blood samples

Impact on canine neutrophil preservation with the addition of bovine serum albumin to K3‐EDTA whole blood samples

Diagnostic and prognostic utility of WBC counts and cell population data in patients with COVID‐19

Single Cell Transcriptome Analysis of Peripheral Blood Mononuclear Cells in Freshly Isolated versus Stored Blood Samples

Contact Info

Product

Resources

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Impact on canine neutrophil preservation with the addition of bovine serum albumin to K₃‐EDTA whole blood samples

Impact on canine neutrophil preservation with the addition of bovine serum albumin to K₃‐EDTA whole blood samples