Assessment of Therapeutic Tumor Response Using 99mTc-Ethylenedicysteine-Glucosamine

Yang, David J.; Yukihiro, Masashi; Yu, Dongfang; Ito, Megumi; Oh, Chang Sok; Kohanim, Saady; Azhdarinia, Ali; Kim, Chang Guhn; Bryant, Jerry; Kim, E. Edmund; Podoloff, Donald A.

doi:10.1089/cbr.2004.19.443

Cited by 33 publications

(9 citation statements)

References 33 publications

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“…Tc-glucosamine by labeling sterile glucosamine (AZPA International, Yantai Dongchong Biochemical Co. Ltd.; Shandong, China) using the method described by Yang et al (4,5) The radiochemical purity was >98%. A 400-MBq dose of 99m Tc-glucosamine (containing 1-2 mg glucosamine) in a bolus of 0.5 mL of normal saline was intravenously administered to each patient via a cannula using the vein in either cubital fossa over 15 s and was flushed with 0.9% normal saline.…”

Section: Radiochemists At Westmead Hospital Produced 99mentioning

confidence: 99%

Evaluating disease activity in patients with ankylosing spondylitis and rheumatoid arthritis using 99mtc-glucosamine

Manolios

Camden

et al. 2016

Eur J Rheumatol

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Objective: To evaluate the clinical utility of a novel radiotracer, Tc-glucosamine, in assessing disease activity of both rheumatoid arthritis (RA) and ankylosing spondylitis (AS).Material and Methods: Twenty-five patients with RA (nine males and 16 females) and 12 patients with AS (all male) at various stages of disease were recruited for the study. A clinical history and examination was performed, followed by the measurement of hematological, biochemical, and autoimmune serological parameters to assess disease activity. Tc-glucosamine was intravenously administered and scans were compared with other imaging modalities, including plain X-ray, magnetic resonance imaging (MRI), and bone scans. Results: In patients with AS, 99mTc-glucosamine scans were more capable of identifying active disease and differentiating between inflammatory and non-inflammatory causes. In patients with RA, 99m Tc-glucosamine accumulated at all known sites of disease involvement. Uptake was most pronounced in patients with active untreated disease. The relative tracer activity in the involved joints increased with time compared with that in the adjoining soft tissue, liver, and cardiac blood pool. Using Spearman's correlation coefficient, there was a positive correlation among glucosamine scan scores, C-reactive protein (p=0.048), and clinical assessment (p=0.003), which was not noted with bone scans. Conclusion:The radiotracer was well tolerated by all patients, with no adverse reactions. Tc-labeled methylene diphosphonate nuclear bone scans for imaging inflamed joints and had the added advantage of demonstrating a significant clinical correlation between disease activity and scan findings.Keywords: Glucosamine, rheumatoid arthritis, ankylosing spondylitis, nuclear scan ments of cells at these sites. Tc-glucosamine can be locally produced by a nuclear scientist in a major nuclear medicine department and administered to a patient, as one would for a bone scan, and it is easy to produce, cheap to administer, and accessible. Other similar analogs of glucosamine, such as N-[2,4,5-trihydroxy-6-(hydroxymethyl) tetrahydro-2H-pyran-3-yl] acrylamide, have been demonstrated to have anti-arthritic and immunomodulatory effects in adjuvant-induced arthritis but are yet to be tested in humans as imaging agents (9).In this study, we extended our prior pilot investigations and evaluated 99m Tc-glucosamine imaging modality for its clinical utility, limitations, and applications, particularly in patients with rheumatoid arthritis (RA) and ankylosing spondylitis (AS). Tc-glucosamine joint uptake was correlated with MRI, bone scans, and in three cases, the effect of anti-tumor necrosis factor (anti-TNF) treatment on scanning before and after therapy. Material and MethodsEthics approval All patients provided their written informed consent. The Western Sydney Local Health District Human Research Ethics Committee granted ethics approval for this study. Study populationPatients fulfilling the American College of Rheumatology revised criteria for...

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Section: Radiochemists At Westmead Hospital Produced 99mentioning

confidence: 99%

Evaluating disease activity in patients with ankylosing spondylitis and rheumatoid arthritis using 99mtc-glucosamine

Manolios

Camden

et al. 2016

Eur J Rheumatol

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“…These glucose membrane transporters are involved in both the glycolytic pathway and the hexosamine biosynthetic pathway. Cell cycle analysis revealed that 99m Tc-ECG was able to transport across the nucleus membrane and involved in proliferation activity in all phases cell cycles [14]. Moreover, the thymidine incorporation assay studies showed similar uptake patterns for both unlabeled ECG and glucose, suggesting that both ECG and glucose were involved in the proliferation/growth activity of cells, whereas unlabeled FDG was not [14].…”

Section: Discussionmentioning

confidence: 99%

Molecular Imaging of Mesothelioma withT99mc-ECG andG68a-ECG

Zhang

Bryant

Kong

et al. 2012

Journal of Biomedicine and Biotechnology

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We have developed ethylenedicysteine-glucosamine (ECG) as an alternative to 18F-fluoro-2-deoxy-D-glucose (18F-FDG) for cancer imaging. ECG localizes in the nuclear components of cells via the hexosamine biosynthetic pathway. This study was to evaluate the feasibility of imaging mesothelioma with 99mTc-ECG and 68Ga-ECG. ECG was synthesized from thiazolidine-4-carboxylic acid and 1,3,4,6-tetra-O-acetyl-2-amino-D-glucopyranose, followed by reduction in sodium and liquid ammonia to yield ECG (52%). ECG was chelated with 99mTc/tin (II) and 68Ga/69Ga chloride for in vitro and in vivo studies in mesothelioma. The highest tumor uptake of 99mTc-ECG is 0.47 at 30 min post injection, and declined to 0.08 at 240 min post injection. Tumor uptake (%ID/g), tumor/lung, tumor/blood, and tumor/muscle count density ratios for 99mTc-ECG (30–240 min) were 0.47 ± 0.06 to 0.08 ± 0.01; 0.71 ± 0.07 to 0.85 ± 0.04; 0.47 ± 0.03 to 0.51 ± 0.01, and 3.49 ± 0.24 to 5.06 ± 0.25; for 68Ga-ECG (15–60 min) were 0.70 ± 0.06 to 0.92 ± 0.08; 0.64 ± 0.05 to 1.15 ± 0.08; 0.42 ± 0.03 to 0.67 ± 0.07, and 3.84 ± 0.52 to 7.00 ± 1.42; for 18F-FDG (30–180 min) were 1.86 ± 0.22 to 1.38 ± 0.35; 3.18 ± 0.44 to 2.92 ± 0.34, 4.19 ± 0.44 to 19.41 ± 2.05 and 5.75 ± 2.55 to 3.33 ± 0.65, respectively. Tumor could be clearly visualized with 99mTc-ECG and 68Ga-ECG in mesothelioma-bearing rats. 99mTc-ECG and 68Ga-ECG showed increased uptake in mesothelioma, suggesting they may be useful in diagnosing mesothelioma and also monitoring therapeutic response.

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“…Ethylenedicysteine (EC) is the most recent and successful example of N 2 S 2 chelates (18–21). EC can be labeled with metallic isotopes easily and efficiently with high radiochemical purity and stability (12–14).…”

Section: Discussionmentioning

confidence: 99%

Molecular imaging of Bcr-Abl phosphokinase in a xenograft model

Yang²,

Angelo³

et al. 2009

Molecular Cancer Therapeutics

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Assessment of Therapeutic Tumor Response Using 99mTc-Ethylenedicysteine-Glucosamine

Abstract: Purpose: The aim of this study was to evaluate 99m Tc-ethylenedicysteine-glucosamine (EC-DG)

Cited by 33 publications

References 33 publications

Evaluating disease activity in patients with ankylosing spondylitis and rheumatoid arthritis using 99mtc-glucosamine

Evaluating disease activity in patients with ankylosing spondylitis and rheumatoid arthritis using 99mtc-glucosamine

Molecular Imaging of Mesothelioma withT99mc-ECG andG68a-ECG

Molecular imaging of Bcr-Abl phosphokinase in a xenograft model

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