Assessment of the Utility of the Oral Fluid and Plasma Proteomes for Hydrocodone Exposure

Kaiser, Brooke L. Deatherage; Jacobs, Jon; Schepmoes, Athena; Brewer, Heather M.; Webb‐Robertson, Bobbie‐Jo; Valtier, Sandra; Bebarta, Vikhyat S.; Adkins, Joshua N.

doi:10.1007/s13181-019-00731-0

Cited by 3 publications

(1 citation statement)

References 30 publications

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“…To avoid introducing bias, no affinity depletion was utilized in this study. Instrument analysis was performed using a platform based upon liquid chromatography mass spectrometry (LC-MS) to globally identify and quantify plasma peptide and their corresponding proteins within each sample and species as previously described ( 34 ). Data generated was based upon label free peak intensity utilizing the MaxQuant data analysis pipeline as previously described ( 35 ) providing protein level quantitative data (LFQ values) for subsequent comparisons.…”

Section: Methodsmentioning

confidence: 99%

Multi-Omic blood analysis reveals differences in innate inflammatory sensitivity between species

Gregory,

Han,

et al. 2023

Preprint

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Vertebrates differ greatly in responses to pro-inflammatory agonists such as bacterial lipopolysaccharide (LPS), complicating use of animal models to study human sepsis or inflammatory disorders. We compared transcriptomes of resting and LPS-exposed blood from six LPS-sensitive species (rabbit, pig, sheep, cow, chimpanzee, human) and four LPS-resilient species (mice, rats, baboon, rhesus), as well as plasma proteomes and lipidomes. Unexpectedly, at baseline, sensitive species already had enhanced expression of LPS-responsive genes relative to resilient species. After LPS stimulation, maximally different genes in resilient species included genes that detoxify LPS, diminish bacterial growth, discriminate sepsis from SIRS, and play roles in autophagy and apoptosis. The findings reveal the molecular landscape of species differences in inflammation, and may inform better selection of species for pre-clinical models.One-Sentence SummaryFundamental differences are present in blood of humans, mice and other species that differ in sensitivity to inflammation.

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Section: Methodsmentioning

confidence: 99%

Multi-Omic blood analysis reveals differences in innate inflammatory sensitivity between species

Gregory,

Han,

et al. 2023

Preprint

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Alcohol, Opioid, and Combined Alcohol and Opioid Use Disorders Affect Shared and Unique Pathways: A Proteomic Analysis of Postmortem Brains

Leung

Stertz

Teixeria

et al. 2022

Preprint

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Substance misuse is a major burden for not only patients but the healthcare system. Understanding how substance misuse alters a patient's genome, proteome, and neurocircuitry is an important step toward identifying targets for treatments. In this exploratory study, we performed a proteomic analysis of postmortem brain from individuals with substance misuse disorder (n=29), including alcohol (n=11) and opioid misuse disorder (n=12), or comorbidity with both disorders (n=6), compared to controls (n=12). The results demonstrate that these substances affect both common and different biological pathways compared to controls. Alcohol misuse affects primarily protein translation, rRNA processing, and energy metabolism pathways, while opioid influences G-protein signaling, protein translation, rRNA processing, energy metabolism, and angiogenesis pathways. Alcohol and opioid misuse combined appears to have a synergistic effect on protein expression of the TCA cycle, respiratory transport, mitochondrial function, LGI-ADAM interactions, interleukin signaling, neuronal system changes, and actin polymerization. These findings offer new insights into how substance misuse leads to protein alterations and how combined substance misuse leads to different protein changes compared to single substance misuse.

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Opioid Monitoring in Clinical Settings: Strategies and Implications of Tailored Approaches for Therapy

Rosendo,

Rosado,

Zandonai

et al. 2024

IJMS

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This review emphasises the importance of opioid monitoring in clinical practice and advocates for a personalised approach based on pharmacogenetics. Beyond effectively managing pain, meticulous oversight is required to address concerns about side effects, specially due to opioid-crisis-related abuse and dependence. Various monitoring techniques, along with pharmacogenetic considerations, are critical for personalising treatment and optimising pain relief while reducing misuse and addiction risks. Future perspectives reveal both opportunities and challenges, with advances in analytical technologies holding promise for increasing monitoring efficiency. The integration of pharmacogenetics has the potential to transform pain management by allowing for a precise prediction of drug responses. Nevertheless, challenges such as prominent pharmacogenetic testing and guideline standardisation persist. Collaborative efforts are critical for transforming scientific advances into tangible improvements in patient care. Standardised protocols and interdisciplinary collaboration are required to ensure consistent and evidence-based opioid monitoring. Future research should look into the long-term effects of opioid therapy, as well as the impact of genetic factors on individual responses, to help guide personalised treatment plans and reduce adverse events. Lastly, embracing innovation and collaboration can improve the standard of care in chronic pain management by striking a balance between pain relief and patient safety.

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Assessment of the Utility of the Oral Fluid and Plasma Proteomes for Hydrocodone Exposure

Cited by 3 publications

References 30 publications

Multi-Omic blood analysis reveals differences in innate inflammatory sensitivity between species

Multi-Omic blood analysis reveals differences in innate inflammatory sensitivity between species

Alcohol, Opioid, and Combined Alcohol and Opioid Use Disorders Affect Shared and Unique Pathways: A Proteomic Analysis of Postmortem Brains

Opioid Monitoring in Clinical Settings: Strategies and Implications of Tailored Approaches for Therapy

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