Assessment of the usefulness of bacterial cellulose produced by Gluconacetobacter xylinus E25 as a new biological implant

Kołaczkowska, Magdalena; Siondalski, Piotr; Kowalik, Maciej Michał; Pęksa, Rafał; Długa, Aldona; Zájac, W; Dederko, P.; Kołodziejska, Ilona; Malinowska‐Pańczyk, Edyta; Sinkiewicz, Izabela; Staroszczyk, Hanna; Śliwińska, Agata; Stanisławska, Alicja; Szkodo, Marek; Pałczyńska, Paulina; Jablonski, G; Borman, A.; Wilczek, Piotr

doi:10.1016/j.msec.2018.12.016

Cited by 22 publications

(27 citation statements)

References 16 publications

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“…In the case of the ex-situ composites, the purified BNC film, in its wet jelly-like consistency, was further processed; the description of this process is presented in Section 2.3 .The grammage (i.e., base weight, g/m 2 ) was determined by means of a weight method. Details of the culture, the medium composition, final purification, and sterilization were described in earlier works [ 37 , 38 ]. The efficiency of BNC production was expressed as the ratio of the dry sample per medium volume (E, g/L), and the yield of the biosynthesis (Y, %) was expressed as follows [ 39 ]:

where m BNC is the dry sample weight of BNC, and m n is the weight of the carbon source in the nutrient medium.…”

Section: Methodsmentioning

confidence: 99%

Bionanocellulose/Poly(Vinyl Alcohol) Composites Produced by In-Situ Method and Ex-Situ/Impregnation or Sterilization Methods

2021

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The purpose of the work was to obtain composites based on bionanocellulose (BNC) and poly(vinyl alcohol) (PVA) for specific biomedical and cosmetic applications and to determine how the method and conditions of their preparation affect their utility properties. Three different ways of manufacturing these composites (in-situ method and ex-situ methods combined with sterilization or impregnation) were presented. The structure and morphology of BNC/PVA composites were studied by ATR-FTIR spectroscopy and scanning microscopy (SEM, AFM). Surface properties were tested by contact angle measurements. The degree of crystallinity of the BNC fibrils was determined by means of the XRD method. The mechanical properties of the BNC/PVA films were examined using tensile tests and via the determination of their bursting strength. The water uptake of the obtained materials was determined through the gravimetric method. The results showed that PVA added to the nutrient medium caused an increase in biosynthesis yield. Moreover, an increase in base weight was observed in composites of all types due to the presence of PVA. The ex-situ composites revealed excellent water absorption capacity. The in-situ composites appeared to be the most durable and elastic materials.

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where m BNC is the dry sample weight of BNC, and m n is the weight of the carbon source in the nutrient medium.…”

Section: Methodsmentioning

confidence: 99%

Bionanocellulose/Poly(Vinyl Alcohol) Composites Produced by In-Situ Method and Ex-Situ/Impregnation or Sterilization Methods

2021

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“…In a different vein, Kołaczkowska et al . developed a BC‐based regenerative implant to be used in cardiac and vascular surgeries by preparing several biocomposites of BC/hyaluronic acid/poly(vinyl alcohol) (PVA), using different methods: addition of hyaluronic acid or PVA to the BC culture media; BC impregnation with aqueous solutions of hyaluronic acid or PVA; BC impregnation in a given solution for 2 h at 80 °C; BC impregnation in a given solution and autoclaving it for 20 min at 121 °C.…”

Section: Applicationsmentioning

confidence: 99%

Latest Advances on Bacterial Cellulose‐Based Materials for Wound Healing, Delivery Systems, and Tissue Engineering

Carvalho

Guedes

Sousa

et al. 2019

Biotechnology Journal

120

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Bacterial cellulose (BC) is a nanocellulose form produced by some nonpathogenic bacteria. BC presents unique physical, chemical, and biological properties that make it a very versatile material and has found application in several fields, namely in food industry, cosmetics, and biomedicine. This review overviews the latest state‐of‐the‐art usage of BC on three important areas of the biomedical field, namely delivery systems, wound dressing and healing materials, and tissue engineering for regenerative medicine. BC will be reviewed as a promising biopolymer for the design and development of innovative materials for the mentioned applications. Overall, BC is shown to be an effective and versatile carrier for delivery systems, a safe and multicustomizable patch or graft for wound dressing and healing applications, and a material that can be further tuned to better adjust for each tissue engineering application, by using different methods.

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“…Assessment of the usefulness of bacterial cellulose as a new biological implant of the circulatory system was assessed based on the properties of this material. 18 Resistance to the stretching of BC used as a constructed material of HAB is approx. 22 MPa where resistance to the stretching of pericardium is 12 MPa.…”

Section: Methodsmentioning

confidence: 99%

In Vitro Study of a Stentless Aortic Bioprosthesis Made of Bacterial Cellulose

Dawidowska¹,

Siondalski

Kołaczkowska

2020

Cardiovasc Eng Tech

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Purpose The paper present findings from an in vitro experimental study of a stentless human aortic bioprosthesis (HAB) made of bacterial cellulose (BC). Three variants of the basic model were designed and tested to identify the valve prosthesis with the best performance parameters. The modified models were made of BC, and the basic model of pericardium. Methods Each model (named V1, V2 and V3) was implanted into a 90 mm porcine aorta. Effective Orifice Area (EOA), rapid valve opening time (RVOT) and rapid valve closing time (RVCT) were determined. The flow resistance of each bioprosthesis model during the simulated heart systole, i.e. for the mean differential pressure (ΔP) at the time of full valve opening was measured. All experimental specimens were exposed to a mean blood pressure (MBP) of 90.5 ± 2.3 mmHg. Results The V3 model demonstrated the best performance. The index defining the maximum opening of the bioprosthesis during systole for models V1, V2 and V3 was 2.67 ± 0.59, 2.04 ± 0.23 and 2.85 ± 0.59 cm2, respectively. The mean flow rate through the V3 valve was 5.7 ± 1, 6.9 ± 0.7 and 8.9 ± 1.4 l/min for stroke volume (SV) of 65, 90 and 110 mL, respectively. The phase of immediate opening and closure for models V1, V2 and V3 was 8, 7 and 5% of the cycle duration, respectively. The mean flow resistance of the models was: 4.07 ± 2.1, 4.28 ± 2.51 and 5.6 ± 2.32 mmHg. Conclusions The V3 model of the aortic valve prosthesis is the most effective. In vivo tests using BC as a structural material for this model are recommended. The response time of the V3 model to changed work conditions is comparable to that of a healthy human heart. The model functions as an aortic valve prosthesis in in vitro conditions.

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Assessment of the usefulness of bacterial cellulose produced by Gluconacetobacter xylinus E25 as a new biological implant

Cited by 22 publications

References 16 publications

Bionanocellulose/Poly(Vinyl Alcohol) Composites Produced by In-Situ Method and Ex-Situ/Impregnation or Sterilization Methods

Bionanocellulose/Poly(Vinyl Alcohol) Composites Produced by In-Situ Method and Ex-Situ/Impregnation or Sterilization Methods

Latest Advances on Bacterial Cellulose‐Based Materials for Wound Healing, Delivery Systems, and Tissue Engineering

In Vitro Study of a Stentless Aortic Bioprosthesis Made of Bacterial Cellulose

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