Assessment of the Toxicological Effects of Pesticides and Detergent Mixtures on Zebrafish Gills: a Histological Study

Santos-Silva, Thamyris; Ribeiro, Rosy Iara Maciel de Azambuja; Alves, Stênio Nunes; Thomé, Ralph Gruppi; Santos, Hélio Batista dos

doi:10.1590/1678-4324-2021210198

Cited by 3 publications

(2 citation statements)

References 55 publications

(81 reference statements)

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“…Additionally, considering IMZ's racemic nature, enantioselective absorption should be considered (Li et al, 2019). Another hypothesis to explain the restricted absorption is the potential alteration of skin mucous cells in the gills due to exposure to toxic substances, such as pesticides, resulting in malfunctions of the gill (Haluzova et al, 2010;Santos-Silva et al, 2021).…”

Section: Diffusion Of Pcz and Imz Through The Gillsmentioning

confidence: 99%

Imazalil and prochloraz toxicokinetics in fish probed by a physiologically based kinetic (PBK) model

LY,

De Oliveira,

Chadili

et al. 2023

Preprint

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Azole fungicides are highly suspected endocrine disruptors (ED) and are frequently detected in surface water. Among them, there are prochloraz (PCZ), a reference molecule and imazalil (IMZ), a highly suspected ED. Little is known about their toxicokinetic (TK) behavior in fish. Hence, research suggested that an improved risk assessment could be achieved by gaining insight into their TK behavior. The aim of this study is to understand and model the TK of both substances in different fish species. Two experiences on zebrafish exposed to IMZ and PCZ were performed to address the lack of in vivo TK data. A physiologically based kinetic (PBK) model applied to IMZ and PCZ was developed, capable to model different exposure scenario. The parameters of the PBK model were simultaneously calibrated on datasets reporting internal concentration in several organs in three fish species (original and literature datasets) by Bayesian methods (Monte Carlo Markov Chain). Model predictions were then compared to other experimental data (i.e. excluded from the calibration step) to assess the predictive performance of the model. The results strongly suggest that PCZ and IMZ are actively transported across the gills, resulting in a small fraction being effectively absorbed by the fish. The model’s results also confirms that both molecules are extensively metabolized by the liver into mainly glucuronate conjugates. Overall, the model performances were satisfying, predicting internal concentrations in several key organs. On average, 90% of experimental data were predicted within a two-fold range. The PBK model allows the understanding of IMZ and PCZ kinetics profiles by accurately predicting internal concentrations in three different fish species regardless of the exposure scenario. This enables a proper understanding of the mechanism of action of EDs at the molecular initiating event (MIE) by predicting bioaccumulation in target organs, thus linking this MIE to a possible adverse outcome.

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Section: Diffusion Of Pcz and Imz Through The Gillsmentioning

confidence: 99%

Imazalil and prochloraz toxicokinetics in fish probed by a physiologically based kinetic (PBK) model

LY,

De Oliveira,

Chadili

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Zebrafish (Danio rerio) is a well-established animal model for several research fields, such as genetics, developmental biology, human diseases, and ecotoxicology [1][2][3][4][5]. It has a low maintenance cost, high reproduction rate, short generation time (approximately 3 months), and large number of eggs per spawning, allowing many animals to be studied at once [6].…”

Section: Introductionmentioning

confidence: 99%

The Zebrafish (Danio rerio) as a Model for Studying Voluntary Physical Exercise and its Effects on Behavior and Metabolism

Martins

Carneiro

Virote

et al. 2023

Braz. arch. biol. technol.

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The objective of this study was to develop a viable and low-cost model of voluntary physical exercise that could be applied to studies on metabolism and behavior. 40 male zebrafish (Danio rerio) were studied, divided into two groups: control and voluntary physical exercise. The model consisted of two aquariums connected by a translucent tube and a video camera on the side to measure physical exercise parameters of the animals. The animals showed higher acceleration and maximum speed and had a higher frequency of activity in the light period. In this model of voluntary physical exercise, we observed better performance in locomotor assessment tests, which was not accompanied by increased anxiety or changes in biochemical parameters related to lipid metabolism. Zebrafish responded positively to voluntary physical exercise and this model appears to be a good option for further studies.

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Key Properties for the Toxicity Classification of Chemicals: A Comparison of the REACH Regulation and Scientific Studies Trends

et al. 2022

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In the last half century, the improvements in quality of life owing to the development of the chemical industry are indisputable. However, despite global improvements, there has also been a large increase in pollution at the environmental level and this has caused relevant harmful risks not only to wildlife and the environment but also to human health. In response, governments have begun to regulate and control chemicals to prevent environmental pollution. At the European level, REACH (Registration, Evaluation, Authorization, and Restriction of Chemicals) was created with the aim to protect human/animal health and the environment from chemicals. Additionally, this regulation shows the main experimental tests that are needed to classify a chemical from a physicochemical and toxicological point of view. The main objective of this study is to compare the tests or experiments stipulated by the European REACH regulation with the studies carried out by the scientific community. To obtain this comparison, an exhaustive bibliographic review was carried out, analyzing the physicochemical properties and the (eco)toxicological information established by the European REACH regulation and scientific articles published in the Web of Science (WOS) database. The results obtained indicate that, although there are many authors who conducted tests indicated by the regulation, there are others whose essays or studies are not in line with the regulation; this may be because, on many occasions, the purpose of the information to be obtained is quite different.

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Assessment of the Toxicological Effects of Pesticides and Detergent Mixtures on Zebrafish Gills: a Histological Study

Cited by 3 publications

References 55 publications

Imazalil and prochloraz toxicokinetics in fish probed by a physiologically based kinetic (PBK) model

Imazalil and prochloraz toxicokinetics in fish probed by a physiologically based kinetic (PBK) model

The Zebrafish (Danio rerio) as a Model for Studying Voluntary Physical Exercise and its Effects on Behavior and Metabolism

Key Properties for the Toxicity Classification of Chemicals: A Comparison of the REACH Regulation and Scientific Studies Trends

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