Assessment of the serodiagnostic potential of nine novel proteins from<i>Mycobacterium tuberculosis</i>

Moran, Alison J; Treit, Janice D.; Whitney, Janice L; Abomoelak, Bassam; Houghton, Raymond L.; Skeiky, Yasir A. W.; Sampaio, Diana P.; Badaró, Roberto; Nano, Francis E.

doi:10.1111/j.1574-6968.2001.tb10615.x

Cited by 20 publications

(4 citation statements)

References 16 publications

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“…Combination of antigens for TB diagnosis could be superior to the use of a single, antigen-based assay [25,26]. Thus, the sensitivity and specificity of the multiple-antigen ELISA system was compared to those of the SPR array system.…”

Section: Resultsmentioning

confidence: 99%

Rapid identification of Mycobacterium tuberculosis infection by a new array format-based surface plasmon resonance method

Hsieh

Chang

et al. 2012

Nanoscale Res Lett

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Tubercle bacillus [TB] is one of the most important chronic infectious diseases that cause millions of deaths annually. While conventional smear microscopy and culture methods are widely used for diagnosis of TB, the former is insensitive, and the latter takes up to 6 to 8 weeks to provide a result, limiting the value of these methods in aiding diagnosis and intermediate decisions on treatment. Therefore, a rapid detection method is essential for the diagnosis, prognosis assessment, and recurrence monitoring. A new surface plasmon resonance [SPR] biosensor based on an array format, which allowed immobilizing nine TB antigens onto the sensor chip, was constructed. Simultaneous determination of multiple TB antibodies in serum had been accomplished with this array-based SPR system. The results were compared with enzyme-linked immunosorbent assay, a conventional immunological method. Array-based SPR showed more advantages in providing label-free and real-time detection. Additionally, the high sensitivity and specificity for the detection of TB infection showed its potential for future development of biosensor arrays for TB diagnosis.

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Section: Resultsmentioning

confidence: 99%

Rapid identification of Mycobacterium tuberculosis infection by a new array format-based surface plasmon resonance method

Hsieh

Chang

et al. 2012

Nanoscale Res Lett

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“…bovis –infected cattle [ 18 , 42 , 43 ]. Mb3871 (Brfb), involved in iron storage, and the glycolipoprotein Mb1403 (LprF), involved in the signaling of potassium-dependent osmotic stress, have been found to elicit antibody reactions in sera from tuberculous patients [ 44 , 45 , 48 , 71 , 72 ]. PE25 (Mb2457c) and PPE41 (Mb2456c) are secreted proteins that naturally form a complex.…”

Section: Discussionmentioning

confidence: 99%

“…PE25/PPE41, Mb1403, and Mb3871, detected in sera from patients with tuberculosis [ 37 , 44 , 45 , 48 ], have not, to our knowledge, previously been evaluated as antigens for bTB detection with sera from M . bovis –infected cattle.…”

Section: Discussionmentioning

confidence: 99%

Identification of New Mycobacterium bovis antigens and development of a multiplexed serological bead-immunoassay for the diagnosis of bovine tuberculosis in cattle

Moens,

Filée,

Boes

et al. 2023

PLoS ONE

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Serological assays for bovine tuberculosis diagnosis require the use of multiple Mycobacterium bovis specific antigens to ensure the detection of infected animals. In the present study, identification and selection process of antigens, based on data from published proteomic studies and involving the use of bioinformatics tools and an immuno-screening step, was firstly performed for identifying novel antigens that elicit an antibody response in M. bovis infection. Based on this approach, a panel of 10 M. bovis antigens [with known relevance (MPB70, MPB83, MPB70/83, and ESAT6/CFP10) and novel (Mb1961c, Mb1301c, Mb3871, Mb1403, Mb0592, and PE25/PPE41)] were constructed and thenused to develop a new multiplexed serological assay based on Luminex technology. The performance of the Luminex-bTB immunoassay was evaluated using sera from cattle with known tuberculosis status. Among the proteins whose ability to detect bovine tuberculosis was evaluated for the first time, PE25/PPE41 and Mb1403, but not Mb3871, showed good detection capacity. Following multiple antigen combination, the final Luminex-bTB immunoassay included seven antigens (MPB70, MPB83, MPB70/83, ESAT6/CFP10, PE25/PPE41, Mb1403, and Mb0592) and showed better global performance than the immunoassay using the four usual antigens (MPB70, MPB70/83, MPB83 and ESAT6/CFP10). The specificity and sensitivity values were, respectively, of 97.6% and 42.8% when the cut-off of two-positive antigens was used to classify samples as positive. With the use of the more-restrictive criterion of three-positive antigens, the specificity increased to 99.2% but the sensitivity decreased to 23.9%. The analysis of antigen profiles generated with the Luminex-bTB immunoassay showed that mainly serodominant proteins were recognized in samples from infected cattle. The detection of Mb1961c and Mb1301c appeared to be associated with presumed false-positive results. Moreover, sera from cattle originating from bTB-outbreaks but having inconclusive or negative skin test results were identified as positive by the Luminex-bTB immunoassay and showed an antigen pattern associated with M. bovis infection. The Luminex-bTB immunoassay including seven antigens may be useful as adjunct test for the detection of M. bovis–infected herds, and different cut-offs could be applied according to the bovine tuberculosis epidemiological context.

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“…The means and standard deviations (SD) were calculated for the sera of uninfected healthy donors (n=48) and the cut off for positive response was calculated as greater than the mean plus 3 SD, and for high level responses, as the mean plus 6 SD. The mean of control group (N=48) was compared with the mean of TB patients (BCG vaccinated and non-vaccinated) group (n=48) for the significance by using students T-test [34]. In bacteria, Two-Component Systems (TCS) have been widely used signal transduction devices which are engaged in a multitude of gene regulatory systems that respond to changing growth conditions.…”

Section: Methodsmentioning

confidence: 99%

EVALUATION OF DevR ANTIGEN (MSMEG_5244) AS A PUTATIVE BIOMARKER FOR THE SERODIAGNOSIS OF PERSISTANT Mycobacterium tuberculosis

Radhamahendran¹,

Malathi²,

Jayaraman³

et al. 2013

Int J Mol Biol

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Tuberculosis (TB) is a major health problem in the developing world. 9 million new cases of TB and three million deaths are reported every year around the globe. The management of tuberculosis in the regions of developing countries urgently needs an efficient diagnostic test. DevR (also called DosR) is essential for promoting long-term survival of Mycobacterium tuberculosis under low oxygen conditions in a dormant state and may be responsible for latent tuberculosis in one-third of the world's population. The similarity in sequences of DevR in Mycobacterium smegmatis and Mycobacterium tuberculosis has been already proved and hence Mycobacterium smegmatis is used as a model in present study for studying and evaluating the immunogenic properties of antigen DevR. Elimination of tuberculosis (TB) largely depends upon definitive rapid diagnosis and treatment. Widely used diagnostic methods do not qualify for use in a developing country due to lack of either desired accuracy and cost. In the present study ELISA was used to evaluate the diagnostic potential of an immuno-dominant antigen DevR from 36 BCG vaccinated and 12 non-vaccinated TB patients. 48 samples were collected from healthy control subjects. We have evaluated the diagnosis of tuberculosis, based on detection of anti-IgG antibodies in the TB patient's sera. The mean IgG antibody levels in sera of both BCG vaccinated (n=36) and non-vaccinated (n=12) patients with tuberculosis were significantly higher compared to the healthy donors (p<0.05). The results suggest that DevR could be possible candidate biomarker for effective use in the serodiagnosis of persistant tuberculosis infections.

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Assessment of the serodiagnostic potential of nine novel proteins fromMycobacterium tuberculosis

Cited by 20 publications

References 16 publications

Rapid identification of Mycobacterium tuberculosis infection by a new array format-based surface plasmon resonance method

Rapid identification of Mycobacterium tuberculosis infection by a new array format-based surface plasmon resonance method

Identification of New Mycobacterium bovis antigens and development of a multiplexed serological bead-immunoassay for the diagnosis of bovine tuberculosis in cattle

EVALUATION OF DevR ANTIGEN (MSMEG_5244) AS A PUTATIVE BIOMARKER FOR THE SERODIAGNOSIS OF PERSISTANT Mycobacterium tuberculosis

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