Assessment of the role of cortisol and corticosteroid receptors in epidermal ionocyte development in the medaka (Oryzias latipes) embryos

Trayer, Vincent; Hwang, Pung‐Pung; Prunet, Patrick; Thermes, Violette

doi:10.1016/j.ygcen.2013.09.011

Cited by 28 publications

(24 citation statements)

References 48 publications

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“…DNA sequence genotyping showed that the mr Δ2 mutant allele generates a frameshift at amino acid #42, followed by a missense polypeptide of 7 amino acids that shows no significant similarity to any putative protein in the NCBI database. Wild-type medaka MR has 994 amino acids (108 kDa)18.…”

Section: Resultsmentioning

confidence: 99%

“…Cortisol induces Na + -K + -ATPase in Atlantic salmon and improves salinity tolerance via the GR, whereas DOC and aldosterone (which are ligands for teleost MR but not for GR) had no effect29. Also, Trayer et al 18 and Cruz et al 22 found that antisense knockdown of GR, but not of MR, downregulated differentiation of ionocytes in medaka and zebrafish. In our studies on the osmoregulatory esophagus of teleosts, cortisol appears to have a direct GR-dependent effect on differentiation of the esophageal epithelium during acclimation to different salinities, while DOC does not show any significant effects3031.…”

Section: Discussionmentioning

confidence: 99%

“…Studies using treatment with ligands, analysis of endogenous ligand-MR dynamics and knockdown of MR have led to somewhat contradictory results81822232425. Loss-of-function and KO studies are currently lacking in non-mammalian models.…”

Section: Discussionmentioning

confidence: 99%

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Principal function of mineralocorticoid signaling suggested by constitutive knockout of the mineralocorticoid receptor in medaka fish

Sakamoto

Yoshiki

Takahashi

et al. 2016

Sci Rep

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As in osmoregulation, mineralocorticoid signaling is implicated in the control of brain-behavior actions. Nevertheless, the understanding of this role is limited, partly due to the mortality of mineralocorticoid receptor (MR)-knockout (KO) mice due to impaired Na+ reabsorption. In teleost fish, a distinct mineralocorticoid system has only been identified recently. Here, we generated a constitutive MR-KO medaka as the first adult-viable MR-KO animal, since MR expression is modest in osmoregulatory organs but high in the brain of adult medaka as for most teleosts. Hyper- and hypo-osmoregulation were normal in MR-KO medaka. When we studied the behavioral phenotypes based on the central MR localization, however, MR-KO medaka failed to track moving dots despite having an increase in acceleration of swimming. These findings reinforce previous results showing a minor role for mineralocorticoid signaling in fish osmoregulation, and provide the first convincing evidence that MR is required for normal locomotor activity in response to visual motion stimuli, but not for the recognition of these stimuli per se. We suggest that MR potentially integrates brain-behavioral and visual responses, which might be a conserved function of mineralocorticoid signaling through vertebrates. Importantly, this fish model allows for the possible identification of novel aspects of mineralocorticoid signaling.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Principal function of mineralocorticoid signaling suggested by constitutive knockout of the mineralocorticoid receptor in medaka fish

Sakamoto

Yoshiki

Takahashi

et al. 2016

Sci Rep

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“…Whole-genome duplication in the teleost lineage w350 MYA gave rise to two GR isoforms, GR1 and GR2, with an identical domain structure and many similarities in terms of ligand-and DNA-binding (reviewed by Bury & Sturm (2007) and Li et al (2012)). Differential tissue mRNA expression levels and regulation of expression (Greenwood et al 2003, Stolte et al 2008a, Kiilerich et al 2011a, Trayer et al 2013, together with dissimilar EC 50 values for cortisol (Bury et al 2003, Greenwood et al 2003, Stolte et al 2008b, Kim et al 2011, Li et al 2012, suggest functional differences between these two GR subtypes.…”

mentioning

confidence: 99%

Interaction between the trout mineralocorticoid and glucocorticoid receptors in vitro

Kiilerich¹,

Triqueneaux²,

Christensen³

et al. 2015

Journal of Molecular Endocrinology

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The salmonid corticosteroid receptors (CRs), glucocorticoid receptors 1 and 2 (GR1 and GR2) and the mineralocorticoid receptor (MR) share a high degree of homology with regard to structure, ligand-and DNA response element-binding, and cellular co-localization. Typically, these nuclear hormone receptors homodimerize to confer transcriptional activation of target genes, but a few studies using mammalian receptors suggest some degree of heterodimerization. We observed that the trout MR confers a several fold lower transcriptional activity compared to the trout GRs. This made us question the functional relevance of the MR when this receptor is located in the same cells as the GRs and activated by cortisol. A series of co-transfection experiments using different glucocorticoid response elements (GREs) containing promoter-reporter constructs were carried out to investigate any possible interaction between the piscine CRs. Co-transfection of the GRs with the MR significantly reduced the total transcriptional activity even at low MR levels, suggesting interaction between these receptors. Co-transfection of GR1 or GR2 with the MR did not affect the subcellular localization of the GRs, and the MR-mediated inhibition seemed to be independent of specific activation or inhibition of the MR. Site-directed mutagenesis of the DNA-binding domain and dimerization interface of the MR showed that the inhibition was dependent on DNA binding but not necessarily on dimerization ability. Thus, we suggest that the interaction between MR and the GRs may regulate the cortisol response in cell types where the receptors co-localize and propose a dominant-negative role for the MR in cortisolmediated transcriptional activity.

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“…Teleost species, including medaka, generally possess two paralogous GR genes, gr1 and gr2 (Alsop and Vijayan, 2009;Trayer et al, 2013;Miyagawa et al, 2015). The medaka expressed sequence tag (EST) clones for gr1 (clone ID, oleb52f22; GenBank accession numbers, XM_004076127) and gr2 (clone ID, olgi51k04; GenBank accession numbers, NM_001163133) were obtained from National BioResource Project (NBRP) Medaka (http://www.shigen.nig.ac.jp/ medaka/).…”

Section: Examination Of Sex Differences In Gr Expression In the Brainmentioning

confidence: 99%

Glucocorticoid receptor exhibits sexually dimorphic expression in the medaka brain

Kikuchi

Hosono

Yamashita

et al. 2015

General and Comparative Endocrinology

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Assessment of the role of cortisol and corticosteroid receptors in epidermal ionocyte development in the medaka (Oryzias latipes) embryos

Cited by 28 publications

References 48 publications

Principal function of mineralocorticoid signaling suggested by constitutive knockout of the mineralocorticoid receptor in medaka fish

Principal function of mineralocorticoid signaling suggested by constitutive knockout of the mineralocorticoid receptor in medaka fish

Interaction between the trout mineralocorticoid and glucocorticoid receptors in vitro

Glucocorticoid receptor exhibits sexually dimorphic expression in the medaka brain

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