Assessment of the optimized SkinEthic™ Reconstructed Human Epidermis (RHE) 42 bis skin irritation protocol over 39 test substances

Tornier, Carine; Amsellem, Carole; Fraissinette, Anne de Brugerolle de; Alépée, Nathalie

doi:10.1016/j.tiv.2009.08.023

Cited by 34 publications

(10 citation statements)

References 25 publications

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“…Acute toxicity as sign for a direct irreversible tissue damage and prolonged toxicity combined with the release of IL-1α as sign for 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 skin irritation by plasma treatment were investigated by using the EpiSkin model (Skinethic, Paris, France), which is accepted for full replacement of animal testing [35][36][37]. Experiments were performed and evaluated according to manufacturer's protocols using MTT assay and quantification of IL-1α ELISA (R&D Systems, Wiesbaden, Germany).…”

Section: Reconstructed Epidermis (Episkin)mentioning

confidence: 99%

The topical use of non-thermal dielectric barrier discharge (DBD): Nitric oxide related effects on human skin

et al. 2015

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Section: Reconstructed Epidermis (Episkin)mentioning

confidence: 99%

The topical use of non-thermal dielectric barrier discharge (DBD): Nitric oxide related effects on human skin

et al. 2015

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“…The most prominent skin equivalents are EpiDerm (MatTek, Ashland, MA, USA), Episkin (Episkin, Chaponost, France), Apligraf Organogenesis Inc., MA, USA) and models engineered by Skinethic (Skinethic, Nice, France; Welss et al, 2004). Completed skin irritation test protocols in vitro were developed using EpiDerm (Kandarova et al, 2009) and SkinEthic (Tornier et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Identification of potential biomarkers for predicting acute dermal irritation by proteomic analysis

Zhang

Dai

Zhang

et al. 2011

J of Applied Toxicology

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In vitro alternative tests aiming at replacing the traditional animal test for predicting the irritant potential of chemicals have been developed, but the assessment parameters or endpoints are still not sufficient for analysis. To discover novel endpoints for skin irritation responses, a proteomics approach was used to analyze the protein expression in human keratinocytes exposed to sodium lauryl sulfate in the present study. Among the 20 identified proteins with altered expression, small heat shock protein 27 (HSP27) and superoxide dismutase [Cu-Zn] were down-regulated while cofilin-1 was up-regulated significantly in response to the chemical challenge. Keratinocytes were exposed to acid and basic chemicals for further validation of the proteins. HSP27 displayed the most significant alteration both in mRNA and protein levels, accompanied by nuclear translocation. The irritation also induced an increased production of interleukin-1α in keratinocytes. These findings suggest that these proteins may be combinational biomarkers or additional endpoints for skin hazard assessment. Further investigation into the protein alterations would be helpful for the mechanistic understanding of skin irritation.

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“…The in vitro reconstructed human skin tissue (EpiSkin TM ) method, proposed to replace animal testing for skin corrosivity and skin irritation (Tornier et al, 2010), is based on determining cell via-bility, and cytokine release (IL-1a) as an additional endpoint. The reconstructed 0.38 cm 2 skin inserts were used and the assay was performed according to the manufacturer instructions and proto-col.…”

Section: Skin Irritation Testmentioning

confidence: 99%

Are coffee silverskin extracts safe for topical use? An in vitro and in vivo approach

Rodrigues

Pereira

Pimentel

et al. 2015

Industrial Crops and Products

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Recent changes in regulatory requirements and social views on animal testing have incremented the development of reliable alternative tests for predicting skin and ocular irritation potential of products based on new raw materials. In this regard, botanical ingredients used in cosmetic products are among those materials, and should be carefully reviewed concerning the potential presence of irritant con-stituents. In particular, cosmetic products used on the face, in vicinity of the eyes or that may come in contact with mucous membranes, should avoid botanical ingredients that contain, or are suspected to contain, such ingredients. In this study, we aimed to evaluate the effect of a new cosmetic ingredi-ent, namely, coffee silverskin (CS), with an in vitro skin and ocular irritation assay using reconstructed human epidermis, EpiSkin TM , and human corneal epithelial model, SkinEthics TM HCE, and an in vivo assay. Three different extracts of CS were evaluated. The histology of the models after extracts applica-tions was analysed. The in vitro results demonstrated that extracts were not classified as irritant and the histological analyses proved that extracts did not affect both models structure. The content of caffeine, 5-hydroxymethyl furfural and chlorogenic acid was quantified after the epidermal assay. The in vivo test carried out with the most promising extract (hydroalcoholic) showed that, with respect to irritant effects, these extracts can be regarded as safe for topical application.

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Assessment of the optimized SkinEthic™ Reconstructed Human Epidermis (RHE) 42 bis skin irritation protocol over 39 test substances

Cited by 34 publications

References 25 publications

The topical use of non-thermal dielectric barrier discharge (DBD): Nitric oxide related effects on human skin

The topical use of non-thermal dielectric barrier discharge (DBD): Nitric oxide related effects on human skin

Identification of potential biomarkers for predicting acute dermal irritation by proteomic analysis

Are coffee silverskin extracts safe for topical use? An in vitro and in vivo approach

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