Assessment of the N-nitrosopiperidine formation risk from piperine and piperidine contained in spices used as meat product additives

Mey, Eveline De; Maere, Hannelore De; Dewulf, Lore; Paelinck, Hubert; Sajewicz, Mieczysław; Fraeye, Ilse; Kowalska, Teresa

doi:10.1007/s00217-013-2125-4

Cited by 23 publications

(14 citation statements)

References 25 publications

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“…The chosen mobile phase was composed of methanol:water (70:30, v/v), which presented to be a simple eluent system, in addition to providing a lower amount of organic solvent. This mobile phase is more economical than the used by De Mey et al (2014) (19), which employed 100% methanol as the mobile phase. In another HPLC method (Moorthi et al 2013) (18), the mobile phase was composed of an ortho phosphoric acid aqueous solution and acetonitrile, with a higher flow rate (1.2 mL min -1 ), which implies a more elaborate mobile phase and a higher associated cost.…”

Section: Analytical Methods Validationmentioning

confidence: 89%

“…Initially, for the selection of the ideal mobile phase, some tests were performed aiming to find a simple and low cost eluent system. There are some reports in the literature, but none exclusively about piperine (18)(19)(20). The choice for methanol is due to the low cost in comparison with other solvents, thus 100% methanol and methanol:water in different proportions (95:5, 80:20 and 70:30, v/v) were tested.…”

Section: Analytical Methods Validationmentioning

confidence: 99%

“…Moorthi et al (2013) (18) developed a HPLC method to quantify piperine and curcumine in Eudragit ® E100 nanoparticles. Another HPLC method was developed by De Mey et al (2014) (19) to determine piperine, piperidine and Nnitrosopiperidine in spices such as black and white pepper, paprika, chili pepper, allspice and nutmeg. Gowda et al (2014) (20) validated a highperformance liquid chromatography-electron spray ionization mass spectrometry method to quantify guggulsterones, piperine and gallic acid in commercial samples of Triphala guggulu.…”

Section: Introductionmentioning

confidence: 99%

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Analytical Method by Liquid Chromatography to Assay Piperine Associated in Nanoemulsions

Motta¹,

Schneider²,

Codevilla³

et al. 2018

Drug Anal. Res.

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This work aimed to develop and validate a simple, fast and low cost analytical method for the quantification of the bioactive piperine in nanoemulsions by high performance liquid chromatography with UV detection. Nanoemulsions were prepared by spontaneous emulsification and their physicochemical properties were evaluated. Considering the chromatographic conditions, the mobile phase was composed by methanol:water (70:30, v/v), Gemini® C18 column, and UV detection at 343 nm. The method was linear in the concentration range of 5-50 μg mL-1 (r = 0.9999), specific, precise (repeatability of RSD 0.38 % and intermediate precision of RSD 1.11 %), accurate (101.3 %) and robust. Nanoemulsions showed nanometric droplet size, polidispersity index below 0.11 and negative zeta potential. The piperine content in the samples was 0.99 ± 0.01 mg mL-1. Regarding these features, the analytical conditions proposed in this work were adequate and effective to determine the piperine content in nanoemulsions.

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Section: Analytical Methods Validationmentioning

confidence: 89%

Section: Analytical Methods Validationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Analytical Method by Liquid Chromatography to Assay Piperine Associated in Nanoemulsions

Motta¹,

Schneider²,

Codevilla³

et al. 2018

Drug Anal. Res.

View full text Add to dashboard Cite

show abstract

“…The extraction temperature of 70 C was selected upon the results of a separate optimization experiment carried out within the range from 50 C to 80 C, not described here. [12] Three 24-mL portions of an extract obtained from one and the same spice sample were merged in the 100-mL calibrated volumetric flask and filled up to 100 mL with DCM. This solution was used for the chromatographic quantification of piperine in the investigated spices.…”

Section: Accelerated Solvent Extractionmentioning

confidence: 99%

APPLICATION OF ACCELERATED SOLVENT EXTRACTION (ASE) AND THIN LAYER CHROMATOGRAPHY (TLC) TO DETERMINATION OF PIPERINE IN COMMERCIAL SAMPLES OF PEPPER (PIPER NIGRUML.)

Mey

Maere

Dewulf

et al. 2014

Journal of Liquid Chromatography & Related Technologies

Self Cite

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& In this preliminary study, an attempt was made to develop a simple yet reliable overall approach to quantification of piperine in pepper (Piper nigrum L.), and also in the other piperinecontaining spice compositions. Such an approach can prove useful for rapid screening of the commercially traded pepper (and other spices) in a pulverized form, with piperine as a single adulteration marker. The main features of this approach consist in a rapid (ca. 40 min) and exhaustive (as proved in a recovery experiment) extraction of plant material with use of Accelerated Solvent Extraction (ASE), followed by an easy thin-layer chromatographic quantification of piperine in the obtained extracts. Within the framework of this approach, dichloromethane (DCM) was selected as an extracting agent and the extraction temperature was fixed at 70 C. With use of the piperine standard, the calibration curve was developed and applicability of the proposed approach was tested upon the commercial samples of white and black pepper in a pulverized and peppercorn form. The piperine levels obtained with the commercial pepper samples remained in agreement with the data available from the literature. Moreover, the assumed quantification approach correctly differentiates between the lower piperine levels in an unripe (black) pepper and the higher ones in a ripe (white) pepper, and also between the lower piperine levels extractable from the whole peppercorn and the higher ones extractable from the ground pepper. Now on the basis of this preliminary study, a completely validated method of quantifying piperine in botanical matter with use of the proposed approach can be elaborated.

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“…[1][2][3] The chemistry of transition metal ion with macrocyclic ligands is an interesting field in chemistry and has been the subject of extensive research due to their potential applications in different fields, such as coordination chemistry, environmental chemistry and the role they play as models for metalloproteinase and enzymes binding sites in biological systems. [3][4][5][6][7][8][9][10][11] Macrocyclic ligands represent an interesting class of ligands in compared with acyclic multidentate ligands. Macrocyclic complexes show thermodynamic and kinetic stabilities and the characteristic properties inaccessible to the complexes of non-cyclic chelate ligands.…”

Section: Introductionmentioning

confidence: 99%

Kinetics and Mechanism of the Ligand Exchange Reaction Between Tetraaza Macrocycle Ligand and Cu(II) Tetradentate Amine-Amide Complexes

Vafazadeh

Zare-Sadrabadi

2015

ACSi

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The kinetics of the ligand exchange reaction of tetraaza macrocycle, teazma (teazma is 5,7,7,12,14,14-hexamethyl-1,4,8,11-tetraazacyclotetradeca-4,11-diene dihydrogen perchlorate) with Cu(bcen) 2+ and Cu(bctn) 2+ , where bcen and bctn are N,N'-bis(β-carbamoylethyl) ethylendiamine) and N,N'-bis(β-carbamoylethyl) propylendiamine), respectively, have been studied by visible spectrophotometry in dimethylformamide, DMF, solvent at 25 ± 0.2 °C. In the system of Cu(bctn) 2+ /teazma, the ligand exchange reaction proceeds in a two-step-consecutive manner, with two rate constants k bctn obsd (1) and k bctn obsd (2). The first reaction step was dependent on the concentration of teazma macrocycle, while the second reaction step was independent. However, it is found that the ligand exchange reaction in Cu(bcen) 2+ /teazma proceeds in an one-step with the rate constant k bcen obsd. The rate constant is dependent on [teazma] macrocycle. The ligand exchange reaction in the system of Cu(bcen) 2+ /teazma is not complete and after some progress, the reaction reaches equilibrium. On the basis of results, a reaction mechanism is proposed and discussed for the ligand exchange rate.

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Assessment of the N-nitrosopiperidine formation risk from piperine and piperidine contained in spices used as meat product additives

Cited by 23 publications

References 25 publications

Analytical Method by Liquid Chromatography to Assay Piperine Associated in Nanoemulsions

Analytical Method by Liquid Chromatography to Assay Piperine Associated in Nanoemulsions

APPLICATION OF ACCELERATED SOLVENT EXTRACTION (ASE) AND THIN LAYER CHROMATOGRAPHY (TLC) TO DETERMINATION OF PIPERINE IN COMMERCIAL SAMPLES OF PEPPER (PIPER NIGRUML.)

Kinetics and Mechanism of the Ligand Exchange Reaction Between Tetraaza Macrocycle Ligand and Cu(II) Tetradentate Amine-Amide Complexes

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