Assessment of the molluscicidal activity of wormwood (Artemisia dubia, Wallich) leaves ethanolic extract on Oncomelania hupensis quadrasi, Möllendorff

Abstract

Material and methods Collection of wormwood leavesThe plants were collected from Barangay Gabas, Baybay City, Leyte, Philippines during the growth stage between in April and May and were identified by a botanist, Dr. Beatriz S. Belonias of the Department of Biological Sciences, College of Arts and Sciences, Visayas State University. The identification of plants was based on morphological keys as formerly described by previous studies [6,7]. The dirt and debris from the leaves were washed with running tap water. After washing, the leaves were air-dried at room temperature for 3 days [8]. Dried leaves were chopped finely into manageable pieces, ground finely, and airdried again at room temperature for 7 days to remove the remaining moisture content of the leaves [9]. Extraction of bioactive compoundsGround dried leaves were completely submerged with ethanol for infusion for 48 h. After infusion, the preparation was filtered using a funnel topped with filter paper. The filtrate was placed in a beaker and the plant leaf residue was discarded. The filtrate was concentrated in a rotary evaporator at 40 °C and recovered 1/3 of its original volume. The recovered concentrated extract of wormwood