Assessment of the level of seminal zinc and fructose concentration in seminal plasma of Vietnamese infertile men

Trang, Nguyen Thi; Sang, Trieu Tien; Hoàng, Nguyễn Huy; Khanh, Nguyen Tan Gia; Duc, Tran Trung

doi:10.15406/mojboc.2018.02.00079

Cited by 6 publications

(9 citation statements)

References 41 publications

(48 reference statements)

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“…Fructose is reported to play an imperative role in sperm motility and concentration. Fructose is the main carbohydrate created in seminal plasma and looks crucial for sperm motility [28]. The result of seminal plasma fructose assessment indicates that the level of fructose concentration in asthenozoospermic patients was nonsignificantly decreased than in normozoospermic.…”

Section: Discussionmentioning

confidence: 98%

“…The fructose measurement may help in assessing the diagnosis and the management of male infertility. Fructose seminal concentration has negative correlations with sperm concentration and motility [28]. Fructose was measured according to the protocol of Foreman et al [29], which forms a pink color when heated with resorcinol in the presence of hydrochloric acid, which can be directly measured photometrically.…”

Section: Measurement Of Fructose In Semenmentioning

confidence: 99%

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Assessment of correlation between asthenozoospermia and mitochondrial DNA mutations in Egyptian infertile men

Elrahman

Makawy

Hassanane

et al. 2021

Journal of Genetic Engineering and Biotechnology

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Background Asthenozoospermia is a chief reason for male seminal pathologies with an impression of around 19% of infertile patients. Spermatozoa mitochondrial DNA variations seem to link with low sperm motility. The objective of the study was to assess the relation between mitochondrial mutations and male sterility, especially in asthenozoospermia. The patient semen samples were investigated by studying the sperm physical characters; motility, viability, and morphological parameters were then classified into normozoospermia and asthenozoospermia. In addition, the level of malondialdehyde (MDA) as a bio-indicator of lipid peroxidation, seminal fructose, and total antioxidant capacity (TAC) were estimated. For molecular analysis, DNA from the semen samples was extracted using a DNA extraction kit. ND1, ND2, and ATPase6 genes were amplified by using a specific primer. After the purification procedure, each PCR product was sequenced to identify the single nucleotide polymorphisms (SNPs) in selected genes. Results A significant negative correlation between seminal plasma malondialdehyde levels and sperm motility was detected. Meanwhile, TAC analysis revealed significantly lower activity (p ≤ 0.05) in the sample of asthenozoospermic than in normozoospermic men. As regards the seminal plasma fructose, there was no significant difference in the fructose level of normozoospermia and asthenozoospermia cases. At the molecular level, 31 diverse nucleotide substitutions were recognized in mitochondrial DNA. Only ten (10) mutations led to amino acid transformation: four have deleterious effects, four are benign, and the other two have conflicting effectiveness. Conclusions This study is the first in Egypt that is concerned with studying the relationship between the mitochondrial DNA mutations in human spermatozoa of asthenozoospermic patients and fertility. The results displayed scientific indications evidenced that there is an association between mitochondrial mutations and male infertility.

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Section: Discussionmentioning

confidence: 98%

Section: Measurement Of Fructose In Semenmentioning

confidence: 99%

Assessment of correlation between asthenozoospermia and mitochondrial DNA mutations in Egyptian infertile men

Elrahman

Makawy

Hassanane

et al. 2021

Journal of Genetic Engineering and Biotechnology

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“…Zinc has a protective effect on spermatozoa from bacteria and is important in testicular development and spermatogenesis [9][10][11]. In seminal plasma, fructose is mainly produced by the seminal vesicles [12] and has a role in spermatozoa metabolism [13] as a major energy source [14] for motility [13]. The determination of seminal fructose is useful in cases of inflammation of accessory glands and in cases of obstructive azoospermia.…”

Section: Constitution and Functions Of Seminal Plasmamentioning

confidence: 99%

Antioxidants Present in Reproductive Tract Fluids and Their Relevance for Fertility

et al. 2021

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Nowadays, infertility is classified as a disease of the reproductive system. Although it does not compromise the life of the individual, it can have detrimental effects on the physiological and psychological health of the couple. Male fertility evaluation is mainly focused on the analysis of sperm parameters. However, the ejaculated fluid is also composed of seminal plasma, and the study of this fluid can provide crucial information to help in the assessment of male fertility status. Total antioxidant capacity of the seminal plasma has been positively correlated with the fertility of men. Moreover, evidence highlights to a similar importance as that of female reproductive tract fluid antioxidant capabilities and female fertility. Herein, we describe the functions of seminal plasma and female reproductive tract fluids, as well as their main antioxidant components and their relationships with fertility outcomes. Additionally, this review contains the most up to date information regarding the mechanisms of the interaction between the male and the female reproductive fluids and the importance of proper antioxidant capacity for fertilization.

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“…Gonzales GF et al reported that a negative correlation between seminal plasma fructose concentration and motile sperm concentration [10,11]. However, other studies have also shown that fructose concentrations in seminal plasma of patients with oligoz oospermia and azoospermia did not decrease as compared to normal men.…”

Section: Introductionmentioning

confidence: 99%

Complete the Manufacturing Procedure of Kit for Determination of Fructose Concentration in Seminal Fluid for Diagnosis Male Infertility

Trang

2018

BJSTR

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Introduction:Fructose is formed in the seminal vesicle under the action of testosterone and is secreted along with the vas deferens during each ejaculation, so fructose is considered a biochemistry that faithfully reflects the function of these components. Fructose concentrations in normal semen confirm the role of testosterone and the function of normal seminal vesicles, without the phenomenon of obstruction. Materials and Methods:The semen samples were obtained from 300 male partners of infertile couples who attended the Department Biomedical and Genetics of the Hanoi medical university and the semen samples were analyzed for the routine seminal parameters. Fructose concentration was assessed using spectrophotometry (improved ROE method).Results: Optimal solution concentration of TCA is 10%. Composite color composition include 2,5 ml of HCL 30% and 0,25 ml of resorcinol 0,1%; Repeatability: CV% = 1, 407 % <5%; Intermediate precision: CV% = 2.032 % < 5%; Trueness: tex= 0,906

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Assessment of the level of seminal zinc and fructose concentration in seminal plasma of Vietnamese infertile men

Cited by 6 publications

References 41 publications

Assessment of correlation between asthenozoospermia and mitochondrial DNA mutations in Egyptian infertile men

Assessment of correlation between asthenozoospermia and mitochondrial DNA mutations in Egyptian infertile men

Antioxidants Present in Reproductive Tract Fluids and Their Relevance for Fertility

Complete the Manufacturing Procedure of Kit for Determination of Fructose Concentration in Seminal Fluid for Diagnosis Male Infertility

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