The aim of the investigations was to determine an impact of heavy metals bound with deposited PM on contamination degree and related toxicological effects by calculating enrichment indices, namely: geo-accumulation index (Igeo), contamination factor (CF), enrichment factor (EF), as well as ecological risk index (ERI) and modified hazard quotient (mHQ). Calculations were based on the selected element concentrations determined in deposited PM samples in Krakow. The results of the investigations revealed that deposited PM was enriched in heavy metals. As Igeo index provides information of the level of metal accumulation it was stated that deposited PM was practically uncontaminated with Be, Cd, and Tl (class 0) but heavily to extremely contaminated (class 5) with Co and Sn and extremely contaminated (class 6) with As, Ba, Cr, Cu, Li, Mn, Ni, Pb, Sr, Ti, V and Zn. On the other hand, calculated values of CF revealed very high contamination of deposited PM with Cd and Zn, considerable contamination with Sn, Pb, and As, and moderate contamination with Cu and Li. Values of calculated EF revealed that from investigated elements only Zn originated from anthropogenic sources. For Cd small influence of anthropogenic sources was observed. For Pb and Sn non-crustal sources of emission were expected. Calculated ERI values indicated very high for Cd and considerable for Zn potential ecological risk, as well as low potential ecological risk for As, Co, Cr, Cu, Ni, Pb and Tl. Moreover, calculated mHQ values indicated extreme for Zn, considerable for Cr, and moderate for As, Cu and Pb severity of contamination. The analysis revealed that the impact of atmospheric and re-suspended PM on inhabitants constitutes the complex effect of mixture of heavy metals affecting simultaneously the human health.