Assessment of the individual and mixture toxicity of cadmium, copper and oxytetracycline, on the embryo-larval development of the sea urchin Paracentrotus lividus

Gharred, Tahar; Jebali, Jamel; Belgacem, Mariem; Mannai, Rabeb; Achour, Sami

doi:10.1007/s11356-016-6988-3

Cited by 17 publications

(10 citation statements)

References 41 publications

(42 reference statements)

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“…Recently, anthropogenic activities increased its presence in marine environments. Various negative effects of copper on the reproduction of some marine organisms, such as the copepod Tigriopus fulvus (Biandolino, Parlapiano, Faraponova, & Prato, ), the killifish Nothobranchius furzeri (Philippe et al, ), and the sea urchin Paracentrotus lividus (Gharred, Jebali, Belgacem, Mannai, & Achour, ), has been reported. Moreover, copper causes also zooplankton diapause production (Aránguiz‐Acuña & Pérez‐Portilla, ).…”

Section: Discussionmentioning

confidence: 99%

Molecular effects of copper on the reproductive system of mytilus galloprovincialis

Lettieri

Mollo

Ambrosino

et al. 2019

Molecular Reproduction Devel

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This study aims to assess the effects induced by 24 hr exposure to a subtoxic copper concentration on the reproductive system (gonads, spermatozoa, and protamine-like[PL] proteins) of Mytilus galloprovincialis. Inductively coupled plasma-mass spectrometry indicated accumulation of this metal in gonads, spermatozoa, and PL proteins of exposed mussels. Further, real-time polymerase chain reaction analyses showed altered expression levels of mt10 and PL proteins genes in spermatozoa and gonads, respectively, of exposed mussels. Protamine-like proteins, which represent the main basic component of sperm chromatin of this organism, showed a higher DNA binding affinity and a different DNA binding mode in exposed mussels. Moreover, an increased amount of NaCl was required for the release from sperm nuclei of PL-III, the main PL protein component. Finally, PL proteins extracted from exposed mussels promoted DNA oxidative damage in the presence of H 2 O 2. These results demonstrate that the tolerable copper amount could also affect the properties of PL proteins and determine the negative effects on the reproductive system of this organism. These analyses could be useful to develop quick and efficient chromatin-based genotoxicity tests for pollution biomonitoring programs.

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Section: Discussionmentioning

confidence: 99%

Molecular effects of copper on the reproductive system of mytilus galloprovincialis

Lettieri

Mollo

Ambrosino

et al. 2019

Molecular Reproduction Devel

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“…At higher concentrations, we observed both abnormal and delayed development, mostly between the morula and gastrula stages, although in different relative proportion, depending upon the molecule and the concentration. Of the developmental anomalies triggered by all three chemicals, we mainly observed crossed spicules, which are among the most regularly reported malformations in sea urchin larvae (Gharred et al 2016). Interestingly, TBAA-induced mortality was detected early, since we observed a black-coloured dead eggs (unpublished data).…”

Section: Resultsmentioning

confidence: 57%

“…Reproduction is easily performed in vitro, resulting in high numbers of translucid larvae. Sea urchin larvae have been extensively used to assess the embryo-toxicity and embryo genotoxicity of molecules (Hose 1985;McGibbon and Moldan 1986;Gharred et al 2016;Morroni et al 2016;Trifuoggi et al, 2017;Neves et al, 2018;Messinetti et al, 2018;Periera et al, 2018;Dorey et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Assessment of individual and mixed toxicity of bromoform, tribromoacetic-acid and 2,4,6 tribromophenol, on the embryo-larval development of Paracentrotus lividus sea urchin

Lebaron

Mechiri

Richard

et al. 2019

Environ Sci Pollut Res

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Water chlorination is the most widely used technique to avoid microbial contamination and biofouling. Adding chlorine in bromide-rich waters leads to the rapid oxidation of bromide ions and leads to the formation of brominated disinfection by products (bromo-DBPs) that exert adverse effects on various biological models. Bromo-DBPs are regularly encountered within industrialized embayments, potentially impacting marine organisms. Of these, bromoform, tribromoacetic acid and tribromophenol are among the most prevalent. In the present study, we tested the potential toxicity and genotoxicity of these disinfection by-products, using sea urchin, Paracentrotus lividus, embryos. We highlighted that tribromophenol showed higher toxicity compared to bromoform and tribromoacetic acid. Furthermore, a synergistic effect was detected when tested in combination. Pluteus cells exposed for 1h to mixtures of DPBs at several concentrations demonstrated significant DNA damage. Finally, when compared to a non-exposed population, sea urchins living in a bromo-DPB-polluted area produced more resistant progenies, as if they were locally adapted. This hypothesis remains to be tested in order to better understand the obvious impact of bromo-DBPs environments on marine wildlife.

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“…Several treatments have been made on different stages (gametes, blastula, gastrula, and pluteus) showing that concentrations of CdCl 2 of 10 −8 -10 −3 M are able to induce skeletal injuries, producing abnormal embryos and larvae (Pagano et al 1982). Treatments of sperms of P. lividus with Cd at the concentration of 10, 100, 200, and 400 μg/L provoked alterations in the egg fertilization rate and abnormal embryogenesis (alterations on length of spicules, crossed spicules, separated spicules, atrophy of arms, fractured ectoderm and fused arms and blockage of development at different stages: fertilization, segmentation, gastrulation, and prepluteus stage) (Gharred et al 2016). Retarded, malformed larvae and developmental arrest were observed in embryos treated since the pluteus stage with 1000-2500 μg/L of Cd (larvae affected in skeletal or gut differentiation, larvae with normal shape and symmetry, but with reduced size) (Manzo et al 2010).…”

Section: Reproduction and Developmentmentioning

confidence: 99%

“…1 Overview of effects induced by Cd stress in P. lividus embryos and larvae. A) Effect on fertilization (Gharred et al 2016). B) Developmental delays (Roccheri et al 2004).…”

Section: Oxidative Stressmentioning

confidence: 99%

Cadmium stress effects indicating marine pollution in different species of sea urchin employed as environmental bioindicators

Chiarelli

Martino

Roccheri

2019

Cell Stress and Chaperones

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In recent years, researches about the defense strategies induced by cadmium stress have greatly increased, invading several fields of scientific research. Mechanisms of cadmium-induced toxicity continue to be of interest for researchers given its ubiquitous nature and environmental distribution, where it often plays the role of pollutant for numerous organisms. The presence in the environment of this heavy metal has been constantly increasing because of its large employment in several industrial and agricultural activities. Cadmium does not have any biological role and, since it cannot be degraded by living organisms, it is irreversibly accumulated into cells, interacting with cellular components and molecular targets. Cadmium is one of the most studied heavy metal inductors of stress and a potent modulator of several processes such as apoptosis, autophagy, reactive oxygen species, protein kinase and phosphatase, mitochondrial function, metallothioneins, and heat-shock proteins. Sea urchins (adults, gametes, embryos, and larvae) offer an optimal opportunity to investigate the possible adaptive response of cells exposed to cadmium, since these cells are known to accumulate contaminants. In this review, we will examine several responses to stress induced by cadmium in different sea urchin species, with a focus on Paracentrotus lividus embryos. The sea urchin embryo represents a suitable system, as it is not subjected to legislation on animal welfare and can be easily used for toxicological studies and as a bioindicator of environmental pollution. Recently, it has been included into the guidelines for the use and interpretation of assays to monitor autophagy.

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Assessment of the individual and mixture toxicity of cadmium, copper and oxytetracycline, on the embryo-larval development of the sea urchin Paracentrotus lividus

Cited by 17 publications

References 41 publications

Molecular effects of copper on the reproductive system of mytilus galloprovincialis

Molecular effects of copper on the reproductive system of mytilus galloprovincialis

Assessment of individual and mixed toxicity of bromoform, tribromoacetic-acid and 2,4,6 tribromophenol, on the embryo-larval development of Paracentrotus lividus sea urchin

Cadmium stress effects indicating marine pollution in different species of sea urchin employed as environmental bioindicators

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