Assessment of the Hydrocarbon Degrading Abilities of Three Bioaugmentation Agents for the Bioremediation of Crude Oil Tank Bottom Sludge Contaminated Libyan Soil

Mansur, Abdulatif A.; Adetutu, Eric M.; Makadia, Tanvi H.; Morrison, Paul D.; Ball, Andrew S.

doi:10.12691/ijebb-3-1-1

Cited by 12 publications

(2 citation statements)

References 51 publications

(61 reference statements)

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“…Oil sludge hydrocarbon pollution treatments have been widely done physically, chemically, and biologically. Biodegradation is one of the most cost effective and cheaper ways than physical and chemical methods (Mansur et al, 2015). Biodegradation of hydrocarbons utilizes the role of potential microbes in decomposing hydrocarbons.…”

Section: Introductionmentioning

confidence: 99%

Utilization of bacterial consortium for oil sludge biodegradation using bioslurry method

Ni’matuzahroh¹,

Muslikhah.²,

Oktavitri³

et al. 2019

MJM

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Aims: Oil sludge is one of pollutant sources in the environment. Bacterial abundance, interaction, and compatibility of environmental factors ensure the success of biodegradation. The purpose of this study was to determine the effectiveness of bacterial consortium in degrading oil sludge using bioslurry method. Methodology and results: The research design used was completely randomized design 4×5 with variation of bacterial consortium concentration and incubation time. Composition of contaminant and liquid phase in bioslurry method was 1:9 ratio with aeration, at room temperature. The liquid phase comprises distilled water with the addition of 2% (v/v) of molasses as nutrient for bacterial growth. Bacterial growth was evaluated using the Total Plate Count (TPC) method. Total Petroleum Hydrocarbon (TPH) measurements were evaluated using the gravimetric method while the oil sludge hydrocarbon component was evaluated by Gas Chromatography Mass Spectrophotometry (GCMS). The pH and temperature data were analyzed descriptively while TPC and TPH data were analyzed using Two Way ANOVA (α=0.05). The bacterial consortium could grow on oil sludge hydrocarbon substrate with a range of temperature of 29 °C-32 °C and an optimum pH of 7. Biodegradation of TPH was 70.48% at consortium concentration of 15% in 14 days of incubation. Conclusion, significance and impact of study: Biodegradation of oil sludge using a bacterial consortium by bioslurry method is one of the effective methods to reduce pollutants in the management of oil sludge.

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Section: Introductionmentioning

confidence: 99%

Utilization of bacterial consortium for oil sludge biodegradation using bioslurry method

Ni’matuzahroh¹,

Muslikhah.²,

Oktavitri³

et al. 2019

MJM

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“…HZ01 (Deng et al, 2014), Pseudomonas sp. 4M12 (Mansur et al, 2015), Bacillus methylotrophicus (Chandankere (Chandankere et al, 2014). There are some forms of ready to use microorganisms used to degrade oil as liquid (free cell), immobilized cell and powder as shown in Table 2.…”

Section: Low Cost While Difficult Transportationmentioning

confidence: 99%

DEVELOPMENT OF LIQUID BACTERIAL FORMULATION OF Exiguobacterium sp. AO-11 FOR BIOREMEDIATION OF CRUDE OIL CONTAMINATED SEAWATER

Boubpha

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Bioremediation is an effective, low cost and environmental friendly technique to clean up crude oil-contaminated environment. This study aimed to develop the ready to use liquid bacterial formulation of petroleum hydrocarbon-degrading Exiguobacterium sp. AO-11 which was isolated from marine sediment, for bioremediation of crude oil contaminated-seawater. Strain AO-11 was evaluated for the degradation of crude oil in various environmental conditions. The results revealed that this strain could degrade crude oil in various environmental conditions including pH 6 to 9, salinity 8 to 45.4 ppt, temperature 25 to 37oC and crude oil concentration 0.25 to 1.5% (v/v). In order to reduce the cost of bacterial formulation, agro-industrial byproducts, soybean oil mill dry sludge 15% (w/v) (SB) in 1:4 diluted seawater based medium was used for bacterial cultivation and it could enhance bacterial cell growth from 6.07±0.06 Log CFU/ml up to 8.6±0.02 Log CFU/ml in 9 hours. For preparation of liquid formulation, phosphate buffer was selected as a suitable suspension solution which could preserve AO-11 survival at 86.7±1.4% in 30 days and maintain crude oil degradation in liquid cultivation at more than 80%. Protective agent (PEG 1%) was selected for prolonged storage and it could preserve AO-11 up to 72.4±0.2% and 58.7±0.7% at 4ºC and 30ºC in 60 days, respectively. The preliminary estimation of the costs of AO-11 liquid formulation was at 68 baht/L. Moreover, 30-day stored AO-11 formulation could degrade 0.5% (v/v) crude oil in seawater up to 61±5.2%. This study revealed the potential of using agro-industrial waste based cultivation medium for bacterial cell production. Furthermore, liquid bacterial formulation was developed and it has potential to be used for bioremediation of crude oil-contaminated seawater.

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Effects of the Inoculant Strain Pseudomonas sp. SPN31 nah + and of 2-Methylnaphthalene Contamination on the Rhizosphere and Endosphere Bacterial Communities of Halimione portulacoides

et al. 2017

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The aim of this study is to evaluate the effects of the inoculation of the saltmarsh plant (Halimione portulacoides) with Pseudomonas sp. SPN31 nah+ combined with exposure to 2-methylnaphthalene (2-MtN) on the plant rhizosphere and endosphere bacterial communities as well as on plant health. To achieve this goal, microcosm experiments were set up. Denaturing gradient gel electrophoresis (DGGE) profiles and statistical analysis showed that rhizosphere and endosphere bacterial communities had distinct responses to plant inoculation and/or exposure to 2-MtN. PCR-sequencing analysis of nah genes encoding for 2-MtN degrading enzymes suggested the presence of Pseudomonas sp. SPN31 nah+ in the endosphere of H. portulacoides with 2-MtN contamination. Moreover, a significant effect in the photosynthetic performance of inoculated plants was detected. To conclude, despite the potential beneficial effect of plant inoculation with Pseudomonas sp. SPN31 nah+ endophytic bacteria may have on plant health, no significant effect on the removal of MtN was detected for the level of contamination used in the study.

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Assessment of the Hydrocarbon Degrading Abilities of Three Bioaugmentation Agents for the Bioremediation of Crude Oil Tank Bottom Sludge Contaminated Libyan Soil

Cited by 12 publications

References 51 publications

Utilization of bacterial consortium for oil sludge biodegradation using bioslurry method

Utilization of bacterial consortium for oil sludge biodegradation using bioslurry method

DEVELOPMENT OF LIQUID BACTERIAL FORMULATION OF Exiguobacterium sp. AO-11 FOR BIOREMEDIATION OF CRUDE OIL CONTAMINATED SEAWATER

Effects of the Inoculant Strain Pseudomonas sp. SPN31 nah + and of 2-Methylnaphthalene Contamination on the Rhizosphere and Endosphere Bacterial Communities of Halimione portulacoides

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