Assessment of the genotoxicity of mine-dump material using the Tradescantia-stamen hair (Trad-SHM) and the Tradescantia-micronucleus (Trad-MCN) bioassays

Fomin, Anette; Paschke, Albrecht; Arndt, U.

doi:10.1016/s0027-5107(99)00064-0

Cited by 17 publications

(6 citation statements)

References 24 publications

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“…Such an approach was previously employed for studying pollution in the surroundings of nuclear power plants, 6 oil refineries, 7 solid waste incinerators, 2 mine dumps, 8 and the rubber industry, 9 as well as other pollution sources. 10 During the same period, the spontaneous rate of mutations of Tradescantia samples was determined in a control region in the countryside.…”

Section: Methodsmentioning

confidence: 99%

In Situ Monitoring of the Mutagenic Effects of the Gaseous Emissions of a Solid Waste Incinerator in Metropolitan São Paulo, Brazil, Using the Tradescantia Stamen-Hair Assay

Ferreira¹,

Petrenko

Lobo

et al. 2000

Journal of the Air & Waste Management Association

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The present work was designed to determine the potential genotoxicity at the vicinity of a solid waste incinerator in the metropolitan area of São Paulo, using the Tradescantia stamen-hair bioassay. Experiments were carried out between December 1998 and April 1999 in four regions (40 pots of plants per site) selected on the basis of their pollution levels predicted by theoretical modeling of the dispersion of the incinerator's plume. The exposure sites were defined as follows: highest level (incinerator); a high level (museum) located 1.5 km from the emission point; a moderate level (school, at a distance of 3.5 km from the incinerator); and a control (at Jaguariúna countryside). The difference in genotoxicity among the groups was statistically significant (p < 0.001). The frequency of mutations observed in the countryside was significantly lower [2.25 ± 1.55, mean ± SD (standard deviation)] than that of the sites close to the incinerator. The frequency of mutations measured at the school (3.70 ± 1.36) was significantly lower than that measured at both the museum (4.89 ± 1.12) and the incinerator (5.69 ± 1.34). In conclusion, we found a positive correlation between the spatial distribution of the emissions of the incinerator located in an urban area and the mutagenic events measured by the Tradescantia stamenhair assay. The in situ approach employed in this study was simple, efficient, and of low cost. No air or chemical extraction of pollutants was necessary for genotoxicity testing as required by other assays.

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Section: Methodsmentioning

confidence: 99%

In Situ Monitoring of the Mutagenic Effects of the Gaseous Emissions of a Solid Waste Incinerator in Metropolitan São Paulo, Brazil, Using the Tradescantia Stamen-Hair Assay

Ferreira¹,

Petrenko

Lobo

et al. 2000

Journal of the Air & Waste Management Association

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“…The use of plants as bioindicators of genetic toxicity of environmental pollutants has been reported in several studies (Grant, 1994;Knasmuller et al, 1998). Mutagenic activity of chemicals has been analysed with different plant systems such as Allium cepa (Fiskesjo, 1997), Vicia faba (Koppen and Verschaeve, 1996), Trifolium repens (Citterio et al, 2002), and Tradescantia virginiana (Fomin et al, 1999). Chromosome aberration assays, mutation assays, cytogenetic tests and specific locus mutation assays were performed (Constantin and Nilan, 1982;Tardiff et al, 1994;Marcon et al, 1999) with these systems.…”

Section: Introductionmentioning

confidence: 99%

AFLP analysis as biomarker of exposure to organic and inorganic genotoxic substances in plants

Labra

Fabio²,

Grassi

et al. 2003

Chemosphere

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In recent years several plant species have been in use as bioindicators and several tests have been developed to evaluate the toxicity of environmental pollutants in vegetal organisms. In the present paper Arabidopsis thaliana (L.) Heynh. (ecotype Wassilewskija) was used as bioindicators of two genotoxic substances: potassium dichromate and dihydrophenanthrene. Inhibition of seed germination was observed with both pollutants. AFLP analysis (i) indicated that both substances are genotoxic, (ii) showed that dihydrophenanthrene induces DNA changes in different target sequences than potassium dichromate, (iii) quantified the genotoxic effect using cluster analysis by comparing DNA from treated plants with that of control plants. On the basis of these considerations we suggest that AFLP method is a powerful tool for measuring qualitative and quantitative genotoxic activity due to environmental pollutants. AFLP method can be applied to a wide range of bioindicator organisms and may become a universal methodology to identify target genes for specific genotoxic agents. This could open up possibilities for designing specifically targeted assays and new approaches to risk assessment.

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“…The use of plants as bioindicators of genetic toxicity of environmental pollutants has been reported in several studies (Grant, 1994;Knasmuller et al, 1998;Labra et al, 2003;Valverde and Rojas, 2009;Valavanidis and Vlachogianni, 2010). Mutagenic activity of chemicals has been analysed with different plant systems such as Allium cepa (Fiskesjo, 1997), Vicia faba (Koppen and Verschaeve, 1996), Trifolium repens (Citterio et al, 2002), and Tradescantia virginiana (Fomin et al, 1999). These novel technologies offer added value compared with classical testing with whole organisms because they provide information concerning the molecular basis of exposure "molecular signatures" and act as "early warning" signals, enabling a more robust environmental monitoring than has ever been achieved previously (Mussali-Galante et al, 2013).…”

mentioning

confidence: 99%

Evaluating genotoxic effects of plants exposed to heavy metals and polycyclic aromatic hydrocarbons at dumpsite, mechanic workshop and metal scrap site in Lagos

Mustapha¹,

Njoku²,

Adesuyi³

et al. 2019

Journal of Applied Sciences and Environmental Management

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Soil contamination from heavy metals and polycyclic aromatic hydrocarbons (PAHs) from dumpsite, automobile mechanic workshop and metal scrap sites pose human and ecological health risks. This study assesses the levels of heavy metals and PAHs in soils from these sites and their effects on the DNA yield and fragmentation of native plants around there. The DNA yield and fragmentation were compared to those obtained from non-polluted sites (control). DNA of the plants species, Musa acuminata (banana), Jatropha curcas (Barbados nut), Carica papaya (pawpaw), Axonopus compressus (carpet grass), Sida acuta (wireweed), and Eleucine indica (crabgrass grass) for polluted sites and control were analyzed using Gel electrophoresis. Soil heavy metals; copper (Cu), zinc (Zn), lead (Pb) and chromium (Cr) were evaluated from dumpsite, metal area and mechanic workshop using inductively coupled plasma-atomic emission spectrometry (ICP-AES). Polycyclic aromatic hydrocarbons were also determined from soils of the polluted sites using the GC/MS spectrophotometry. The result obtained from the DNA analysis showed that DNA yield and fragmentation of the polluted plants had higher indices than the controls which shows that heavy metals and polycyclic aromatic hydrocarbons affect the DNA of the plants. The effects of the pollutants on DNA of polluted plants had more smearing in their tissue than non-polluted plants. Heavy metals evaluation showed that zinc level was the highest in all the study sites; dumpsite, metal area and mechanic workshop (5.146 mg/kg, 2.709 mg/kg and 1.990 mg/kg respectively) than the control (p<0.05). Chromium was the least present (0.022 mg/kg, 0.018 mg/kg and 0.002 mg/kg respectively). The results of this study indicate that dumpsite, mechanic workshop and metal scrap sites are potential sources of PAH and toxic metal, which can pose serious human health and ecological risks.

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Assessment of the genotoxicity of mine-dump material using the Tradescantia-stamen hair (Trad-SHM) and the Tradescantia-micronucleus (Trad-MCN) bioassays

Cited by 17 publications

References 24 publications

In Situ Monitoring of the Mutagenic Effects of the Gaseous Emissions of a Solid Waste Incinerator in Metropolitan São Paulo, Brazil, Using the Tradescantia Stamen-Hair Assay

In Situ Monitoring of the Mutagenic Effects of the Gaseous Emissions of a Solid Waste Incinerator in Metropolitan São Paulo, Brazil, Using the Tradescantia Stamen-Hair Assay

AFLP analysis as biomarker of exposure to organic and inorganic genotoxic substances in plants

Evaluating genotoxic effects of plants exposed to heavy metals and polycyclic aromatic hydrocarbons at dumpsite, mechanic workshop and metal scrap site in Lagos

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