Assessment of the genotoxic potential of Lake Skadar sediments using Ames test and comet assay on the fish cell line RTL-W1

Abstract

In this study we evaluated the genotoxic potential of surface sediment extracts of Lake Skadar using a combination of two in vitro tests: the Comet assay on the fibroblast-like permanent cell line RTL-W1, and the Ames test on the strain Salmonella typhimurium TA98. The obtained results show that both tests were successful in determining the genotoxic potential in the sediment organic fractions. They possess enough sensitivity to detect early warning signals and evaluate the genotoxic potential in sediments of the Lake. The genotoxic potential was recorded and compared in the sediment samples from different locations on the Lake Skadar

Guaiazulene: biochemical activity and cytotoxic and genotoxic effects on rat neuron and N2a neuroblastom cells

“…The dried slides were then stained using ethidium bromide (20 lg/mL)after appropriate fixing for 10 min. The whole procedure was carried out in dim light to minimize artifact [ 23 ]. DNA damage analysis was performed at a magnification of ×100 using a fluorescence microscope (Nikon Eclips E6600, Japan)after coding the slides by one observer (Togar B.).…”

Pharmaceuticals and endocrine-disrupting compounds: Accumulation in the tissue of Skadar lake fish

“…In spite of its importance, inflowing waters from the Moraca river and other regional rivers contaminated by the industry, municipal and agricultural activities in the area influence the Lake. Therefore, the Lake has been the subject of various physical, chemical, biological and toxicological examinations (Beeton & Karaman, 1981;Rastall et al, 2004;Rakocevic-Nedovic & Hollert, 2005;Stesevic et al, 2007;Perovic et al, 2012;Kastratovic et al, 2014;Vemic et al, 2014;Petrovic et al, 2016, Perovic et al, 2019. In this study, we assessed the level of bioaccumulation of ECDs and PhACs in aquatic organisms (fish tissues) Rutilus prespensis (Karaman, 1924), Squalius platyceps (Zupancic et al, 2010), Scardinius knezevici (Bianco & Kottelat, 2005), Chondrostoma ohriadunum (Karaman, 1924), Cyprinus carpio (Linnaeus, 1758) and Alburnus scoranza (Bonaparte, 1845).…”