Assessment of the genetic diversity of wild hops (Humulus lupulus L.) in Europe using chemical and molecular analyses

Patzak, Josef; Nesvadba, Vladimír; Henychová, Alena; Krofta, Karel

doi:10.1016/j.bse.2009.12.023

Cited by 57 publications

(43 citation statements)

References 22 publications

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“…Therefore, RAPD-based data are not useful for estimating the chemical characteristics of C. polygonoides samples. Krofta et al (1998), Patzak et al (2010) also found difference between molecular and chemical data of wild hops (Humulus lupulus). High similarity in chemical parameters proposes least variable expression of the genes for these chemical parameters in extreme drought conditions.…”

Section: Discussionmentioning

confidence: 99%

Chemical and genetic diversity among some wild stands of Calligonum polygonoides (Polygonaceae) from the Thar Desert of Rajasthan

Vyas

Kumar

Sharma

et al. 2012

RBT

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Abstract:The arid Western Rajasthan, where the Thar Desert of India is immersed, is mostly covered by sand dunes, a common landscape. The region has confronted with fragilities of natural resources, low, erratic and ill-distributed rainfall, and is covered up with many perennial hardy shrubs. Calligonum polygonoides, the most common perennial shrub, is widely present in some localities of this Thar Desert. In this study, we evaluated the diversity present among 54 wild Calligonum polygonoides plants, sampled from eight different locations within the Thar Desert. Our methods included chemical/nutritional characteristics and random amplified polymorphic DNA (RAPD). Both chemical and molecular methods produced wider range of diversity, however, RAPD detected comparatively more diversity. A total of 163 band positions were produced by ten RAPD primers, of which 147 were found polymorphic with 90.18% polymorphism. RAPD-based Jaccard's similarity coefficients ranged from 0.43-0.89. The analysis of various chemical and mineral constituents revealed that phog is an excellent source of calcium, potassium and phosphorous while relatively poor in zinc. Among minerals, average potassium content was found maximum (2 430mg/100g) with 0.14 CV. Zinc was observed comparably less in quantity while highest variable with CV 0.73. The chemical-based Manhattan dissimilarity coefficient values ranged from 0.01-0.22 with an average of 0.12. The comparison of the clusters obtained based on the chemical and mineral parameters with those of the RAPD data showed that the groups formed in both cases showed different patterns of relationships among the samples. Broader range of diversity might be due to the out breeding behavior of C. polygonoides and indicates the good adaptability of the plants in the region studied. However, low diversity observed in the Bikaner province is alarming and suggests that anthropogenic activities leading to heavy population disturbances can affect the genetic composition of the species in a considerable way. Rev.

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Section: Discussionmentioning

confidence: 99%

Chemical and genetic diversity among some wild stands of Calligonum polygonoides (Polygonaceae) from the Thar Desert of Rajasthan

Vyas

Kumar

Sharma

et al. 2012

RBT

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“…We found that the bitter acid contents were significantly dependent on the phenophases of cones and were also influenced by the harvest year. The harvest year is dependent on the environmental factors (weather conditions, disease infections, pest damage, and plant ontogenesis) (Patzak et al 2010). We also found some significant differences in the contents between varieties.…”

Section: Resultsmentioning

confidence: 59%

Characterisation of hop varieties grown in Romania based on their contents of bitter acids by HPLC in combination with chemometrics approach

Salanță¹,

Tofanä²,

Socaci³

et al. 2015

Czech J. Food Sci.

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Salanţă L.C., Tofană M., Socaci S., Mudura E., Fărcaş A., Pop C., Pop A., Odagiu A. (2015): Characterisation of hop varieties grown in Romania based on their contents of bitter acids by HPLC in combination with chemometrics approach. Czech J. Food Sci., 33: 148-155.Reverse-phase high performance liquid chromatography technique was used for studying the evolution of bitter acids from three varieties of hop growing in Romania during the development of hop cones and the pelletisation process in order to provide the information on the bitter acids profile of each cultivar. Chemometrics methods were applied for highlighting the statistical correlations existing between the genotype (variety), chemotype (composition), and phenotype (phenophase of cone development) with respect to the classes of biologically active compounds investigated (bitter acids). The bitter acid content of each hop cultivar was not only significantly dependent on the phenophases of the cones, but was also influenced by the harvest year. The variations in the α/β ratio as well as cohumulone and colupulone contents were low in both experimental years and the cohumulone/∑α did fraction not exceed 30% in any of the three varieties.

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“…The use of ESTs is a new trend for SSR markers in all main crops (Feingold et al 2005, Hu et al 2009). The possibility to use polymorphism in structural genes was reported by Patzak et al (2007), Bassil et al (2008) and Castro et al (2008). The increasing information about hop DNA gene sequences in GeneBank EST database provides possibility to look for new gene specific molecular markers.…”

Section: ⎯⎯⎯⎯mentioning

confidence: 88%

“…Forty two pairs of primers flanking SSRs were designed using PrimerSelect module (LASERGENE system). Primer pairs were designed to define product sizes ranging from 100 to 500 bp in length ( (Patzak 2002, Patzak et al 2007) Additionally, a female individual of Humulus japonicus, from seed, were used for test of cross-species marker amplification. DNA was isolated from young leaves according to Patzak (2001).…”

Section: ⎯⎯⎯⎯mentioning

confidence: 99%

Development and evaluation of expressed sequence tag-derived microsatellite markers for hop genotyping

Patzak¹,

Matoušek

2011

Biol. plant.

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The use of expressed sequence tag-simple sequence repeat (EST-SSR) markers might reflect the better relationship among species or cultivars than markers previously used. The first set of 30 EST-SSR was developed in hop (Humulus lupulus L.). They represent 25 gene loci with total of 1268 EST sequences. They were used for characterization of 11 hop samples and cross-amplification in Humulus japonicus Sieb. et Zucc. The number of alleles per locus ranged from two to nine. The observed and expected heterozygosities ranged from 0.182 to 0.956 and from 0.233 to 0.775, respectively. We used EST-SSR markers for cluster analysis of hop genotypes. Dendrogram well matched with genealogical and geographical data for hop genotypes.

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Assessment of the genetic diversity of wild hops (Humulus lupulus L.) in Europe using chemical and molecular analyses

Cited by 57 publications

References 22 publications

Chemical and genetic diversity among some wild stands of Calligonum polygonoides (Polygonaceae) from the Thar Desert of Rajasthan

Chemical and genetic diversity among some wild stands of Calligonum polygonoides (Polygonaceae) from the Thar Desert of Rajasthan

Characterisation of hop varieties grown in Romania based on their contents of bitter acids by HPLC in combination with chemometrics approach

Development and evaluation of expressed sequence tag-derived microsatellite markers for hop genotyping

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