Assessment of the feasibility of serological monitoring and on-farm information about health status for the future meat inspection of fattening pigs

Felin, Elina; Hälli, Outi; Heinonen, Mari; Jukola, Elias

doi:10.1016/j.prevetmed.2018.11.009

Cited by 20 publications

(17 citation statements)

References 26 publications

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“…Our findings also support that it is possible to identify animals with health and welfare outcomes on farm and to transport them from farm to abattoir in a separate group ( 13 ), allowing meat inspection procedures to be made more efficient ( 16 ) and to decrease the risk of microbial cross-contamination ( 5 ). Additionally, such animal-based welfare outcomes can be incorporated with food chain information (FCI) ( 29 , 30 ), enabling evidence-based risk categorization of pigs before slaughter ( 31 ).…”

Section: Discussionmentioning

confidence: 99%

Assessment of Animal-Based Pig Welfare Outcomes on Farm and at the Abattoir: A Case Study

2020

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The study assessed the prevalence of animal-based pig welfare outcomes on one Chilean farm and one abattoir. A total of 198 pens of slaughter pigs (9,049 pigs) were observed on farm and 54 batches (8,843 pigs) were observed at the abattoir. All assessments were conducted from outside the pen on farm and from outside the corridor where pigs were unloaded from the truck at the abattoir. Batch size and number of pigs with ear, tail and skin lesions, hernias, rectal prolapse, bursitis, and lameness were recorded. Data were analyzed using generalized linear mixed models. There was a large variation among pens on farm and among batches at the abattoir for all outcomes. Bursitis was the most prevalent outcome recorded in both locations, followed by ear lesions recorded on farm and by tail lesions recorded at the abattoir. Ear lesions' prevalence was higher on farm ( P < 0.001), while tail lesions, hernia, and bursitis prevalence were higher at the abattoir ( P < 0.001). Ear lesions' prevalence on farm was higher in female and mixed-sex groups than in male groups (P < 0.01), but male groups tended to have a higher tail lesions' prevalence ( P < 0.1). The results show a difference in welfare outcomes, suggesting that assessment of outcomes on farm could complement ante-mortem inspections at the abattoir. However, as the same animals were not inspected in the two locations and there is the possibility of a seasonal influence on the results, the findings should be interpreted with caution and further research is required.

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Section: Discussionmentioning

confidence: 99%

Assessment of Animal-Based Pig Welfare Outcomes on Farm and at the Abattoir: A Case Study

2020

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“…Official meat inspection procedures in the European Union are moving from a traditional, macroscopic examination of slaughtered animals towards a more risk based safety assurance system [21]. In view of this development, serological screening for zoonotic agents in pig herds is considered as a potential tool to improve food safety [2,3,22]. Felin et al [3] stated that the best timing for serological screening is at the end of the fattening period or at the abattoir.…”

Section: Discussionmentioning

confidence: 99%

“…In view of this development, serological screening for zoonotic agents in pig herds is considered as a potential tool to improve food safety [2,3,22]. Felin et al [3] stated that the best timing for serological screening is at the end of the fattening period or at the abattoir. This study investigated whether the specific demands of a screening test for pigs at the abattoir, including different sample materials and fast turn-around times, could possibly be met by using a protein microarray.…”

Section: Discussionmentioning

confidence: 99%

“…The Bland-Altman plots showed that the high level of agreement between serum and meat juice was also 1 Antigen spots of T. gondii 0.5, Y. enterocolitica 0.5 and M.hyopneumoniae 1:10 were included in the analysis 2 Antigen spots of T. gondii 0.5, Y. enterocolitica 0.5 and APP 1:5 were included in the analysis 3 The standard deviation (SD) among replicates was calculated from every microarray chip and the mean of these SD values is presented in the table present in the quantitatively measured signal intensities. This confirms that the sample dilutions were chosen appropriately.…”

Section: Number Of Positive and Negative Samples In Roc Analysismentioning

confidence: 99%

“…monitoring programs. Meemken et al [2] and Felin et al [3] showed that continuous serological monitoring for more zoonotic agents could be a meaningful tool for risk categorisation of pig herds and enable targeted control measures at the abattoir. The advantages of serological testing include practicality, ease of sample collection and preparation, high sample throughput, low costs and fast turnaround times [4].…”

Section: Introductionmentioning

confidence: 99%

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Use of meat juice and blood serum with a miniaturised protein microarray assay to develop a multi-parameter IgG screening test with high sample throughput potential for slaughtering pigs

et al. 2020

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Background: Serological screening of pig herds at the abattoir is considered a potential tool to improve meat inspection procedures and herd health management. Therefore, we previously reported the feasibility of a miniaturised protein microarray as a new serological IgG screening test for zoonotic agents and production diseases in pigs. The present study investigates whether the protein microarray-based assay is applicable for high sample throughput using either blood serum or meat juice.Material and methods: Microarrays with 12 different antigens were produced by Abbott (formerly Alere Technologies GmbH) Jena, Germany in a previously offered 'ArrayTube' platform and in an 'ArrayStrip' platform for large-scale use. A test protocol for the use of meat juice on both microarray platforms was developed. Agreement between serum and meat juice was analysed with 88 paired samples from three German abattoirs. Serum was diluted 1:50 and meat juice 1:2. ELISA results for all tested antigens from a preceding study were used as reference test to perform Receiver Operating Characteristic analysis for both test specimens on both microarray platforms.Results: High area under curve values (AUC > 0.7) were calculated for the analysis of T. gondii (0.87), Y. enterocolitica (0.97), Mycoplasma hyopneumoniae (0.84) and Actinobacillus pleuropneumoniae (0.71) with serum as the test specimen and for T. gondii (0.99), Y. enterocolitica (0.94), PRRSV (0.88), A. pleuropneumoniae (0.78) and Salmonella spp. (0.72) with meat juice as the test specimen on the ArrayStrip platform. Cohens kappa values of 0.92 for T. gondii and 0.82 for Y. enterocolitica were obtained for the comparison between serum and meat juice. When applying the new method in two further laboratories, kappa values between 0.63 and 0.94 were achieved between the laboratories for these two pathogens.(Continued on next page) Conclusion: Further development of a miniaturised pig-specific IgG protein microarray assay showed that meat juice can be used on microarray platforms. Two out of twelve tested antigens (T. gondii, Y. enterocolitica) showed high test accuracy on the ArrayTube and the ArrayStrip platform with both sample materials.

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Enteropathogenic Yersinia spp.

Fredriksson-Ahomaa

2022

Zoonoses: Infections Affecting Humans and Animals

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Assessment of the feasibility of serological monitoring and on-farm information about health status for the future meat inspection of fattening pigs

Abstract: Assessment of the feasibility of serological monitoring and on-farm information about health status for the future meat inspection of fattening pigs, Preventive Veterinary Medicine (2018),

Cited by 20 publications

References 26 publications

Assessment of Animal-Based Pig Welfare Outcomes on Farm and at the Abattoir: A Case Study

Assessment of Animal-Based Pig Welfare Outcomes on Farm and at the Abattoir: A Case Study

Use of meat juice and blood serum with a miniaturised protein microarray assay to develop a multi-parameter IgG screening test with high sample throughput potential for slaughtering pigs

Enteropathogenic Yersinia spp.

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