Assessment of the experimental model of transplanted C6 glioblastoma in wistar rats

Abstract

Establishing in vivo glioblastoma models from cell lines requires a very strict methodology, in order to obtain reproducible tumors presenting all the characteristics of human spontaneous glioblastomas. In this respect, we have developed a model of glioblastoma in Wistar rats by stereotaxic intracerebral transplantation of a 10 microliters suspension of 6 x 10(6) C6 cells grown in vitro. The tumor take was very high in these conditions, only 1 rat over 30 had no tumor. The median survival time varied from 14 to 20 days. The growth curve of the tumor has revealed an exponential growth up to the 10th day after transplantation with a doubling time of 36 h. Histological examination of the tumors has shown several characteristic features of spontaneous glioblastomas, such as neovasculature, parenchymal invasion, nuclear pleiomorphism, and presence of hemorrhagic and necrotic areas. This C6 model is closer to the usual histological characteristics of spontaneous glioblastomas when using the Wistar rather than other strain, and it should be used in that way for preclinical therapeutic evaluation of new drugs or drug combinations in glioblastoma.

Experimental nodel of C6 brain tumors in athymic rats

“…These characteristics have led to the use of the C6/wistar model in experiments due to its similarity to the human GBM 5 . despite this similarity, other authors believe this model does not possess the same heterogeneity, immuno-histochemical characteristics or invasive capacity of the GBM 6 .…”

“…some authors have reported that these cells in fact originated from wistar rats 6 , and were chemically induced in wistarFurth rats by exposure to N,N'-nitrosomethylureia 9 . other authors have described C6 cells as compatible with BdIX 10 and sprague-dawley rats 11 .…”

“…Immunosupressed (or athymic) rats are necessary when inoculating cell cultures derived from patient' s tumors. other models are either incompatible, or do not reproduce the invasive characteristics of gliomas [1][2][3][4][5][6][7] . The objective of the present work was to present the technique, along with clinical and pathological results, for an experimental glioma model by injecting murine glioma cells (C6) in athymic rats.…”

125I-Iododeoxyuridine for the Treatment of a Brain Tumor Model: Selection of Conditions for Optimal Effectiveness

“…Transplantation of cultured C6 cells into the brains of rats by stereotaxic procedure produces intra-cerebral tumors representing as closely as possible the characteristics of spontaneous gliomas with good reproducibility [35]. C6/Lac Z cells which constitutively express the LacZ reporter gene were obtained from ATCC.…”

Fourier Transform Infrared Microspectroscopy for Cancer Diagnostic of C6 Glioma on Animal Model

“…Rat C6 glioma cells are an experimental cell line that when injected into neonatal Wistar rats grow into an intracerebral tumor with pathological similarities to human glioblastoma (GBM) (Auer et al, 1981). This glioblastoma model was used in a variety of studies related to brain tumor biology including tumor growth (Nagano et al, 1993;San-Galli et al, 1989), invasion (Nagano et al, 1993;Bernstein et al, 1991;Chicoine & Silbergeld, 1995), and evaluation of the therapeutic efficacy of cancer treatments (Barth 1998). The glioma tumors were obtained by injection of C6 glioma cells suspension in brain parenchyma as described elsewhere (Grobben et al, 2002).…”