“…These findings are similar to another study, which showed that 2% Lysoformin 3000 at 30 min was enough for bactericidal and fungicidal activity (Aksen et al, 2004). It was reported that all chlorine-based disinfectants were significantly more effective in killing bacteria and fungi in 5 min and the efficiency was generally increased when the contact time was prolonged from 5 to 15 min (Van Klingeren et al, 1998;Özalp et al, 2007;Salo and Wirtanen, 2005). Other groups reported that 3-25% hydrogen peroxide was able to inactivate bacteria and fungi by > 6 log 10 reduction in a contact time of 5 min, while, Sanipine and Sanismel were effective only at 1:15 for 30 min requiring prolonged exposure time or greater concentration (Russell, 1998;Alfa and Jackson, 2001;Sattar et al, 2002).…”
Section: Discussionsupporting
confidence: 88%
“…Concerning the bactericidal and fungicidal efficiencies of the tested antiseptics, the results indicate that three of the alcohol based biocides (Alcohol, AHD 2000 and Hospidermin) reduced the viable titers of the suspended vegetative bacteria and fungi by more than 5 log values even at a concentration of 50% and within 30 s. The fourth alcohol based biocide, Sanigel, reached the same efficacy but at the longer contact time of 120 s. Similar findings have been reported by other groups showing that alcohol based antiseptics have a very rapid and effective antimicrobial action that exceeds any other antiseptic at concentration 50-90% for shorter contact times against bacteria and fungi (Van Klingeren et al, 1998;Vieira et al, 2005;Jeng and Severin, 1998). Among the six disinfectants tested, only the aldehydebased disinfectant (Lysoformin 3000), Chlorine-based: (Chlorax and Trichlorol) and Hydrogen peroxide had an excellent killing activity within 5 min against the suspended vegetative bacteria and fungi at the recommended concentrations.…”
The appropriate use of biocides is essential in any vaccine production facility and their proper evaluation using standardized tests marks the first step to ensure their proper use. Quantitative suspension tests against reference and environmental isolates were carried out to evaluate the efficacy of various biocides used in the main vaccine production facility in Egypt. Several use-dilutions of the biocides were evaluated at contact times of up to 5 min in case of antiseptics and 30 min in case of disinfectants to measure the biocide activity against bacteria, fungi and spores. Standard strains were used in addition to the main bacterial isolates identified in an environmental monitoring program carried out in the same facility. Alcohol based biocides showed bactericidal and fungicidal activity but no sporicidal activity. Chlorine based compounds and glutaraldehyde showed bactericidal and fungicidal effect while, the sporicidal effects depended on the used dilution and the contact time. Hydrogen peroxide showed bactericidal, fungicidal and sporicidal activity. Quaternary ammonium compounds tested showed very weak activity in all tests. Evaluation of the biocide is an essential step to guarantee the use of the most appropriate agent in any location.
“…These findings are similar to another study, which showed that 2% Lysoformin 3000 at 30 min was enough for bactericidal and fungicidal activity (Aksen et al, 2004). It was reported that all chlorine-based disinfectants were significantly more effective in killing bacteria and fungi in 5 min and the efficiency was generally increased when the contact time was prolonged from 5 to 15 min (Van Klingeren et al, 1998;Özalp et al, 2007;Salo and Wirtanen, 2005). Other groups reported that 3-25% hydrogen peroxide was able to inactivate bacteria and fungi by > 6 log 10 reduction in a contact time of 5 min, while, Sanipine and Sanismel were effective only at 1:15 for 30 min requiring prolonged exposure time or greater concentration (Russell, 1998;Alfa and Jackson, 2001;Sattar et al, 2002).…”
Section: Discussionsupporting
confidence: 88%
“…Concerning the bactericidal and fungicidal efficiencies of the tested antiseptics, the results indicate that three of the alcohol based biocides (Alcohol, AHD 2000 and Hospidermin) reduced the viable titers of the suspended vegetative bacteria and fungi by more than 5 log values even at a concentration of 50% and within 30 s. The fourth alcohol based biocide, Sanigel, reached the same efficacy but at the longer contact time of 120 s. Similar findings have been reported by other groups showing that alcohol based antiseptics have a very rapid and effective antimicrobial action that exceeds any other antiseptic at concentration 50-90% for shorter contact times against bacteria and fungi (Van Klingeren et al, 1998;Vieira et al, 2005;Jeng and Severin, 1998). Among the six disinfectants tested, only the aldehydebased disinfectant (Lysoformin 3000), Chlorine-based: (Chlorax and Trichlorol) and Hydrogen peroxide had an excellent killing activity within 5 min against the suspended vegetative bacteria and fungi at the recommended concentrations.…”
The appropriate use of biocides is essential in any vaccine production facility and their proper evaluation using standardized tests marks the first step to ensure their proper use. Quantitative suspension tests against reference and environmental isolates were carried out to evaluate the efficacy of various biocides used in the main vaccine production facility in Egypt. Several use-dilutions of the biocides were evaluated at contact times of up to 5 min in case of antiseptics and 30 min in case of disinfectants to measure the biocide activity against bacteria, fungi and spores. Standard strains were used in addition to the main bacterial isolates identified in an environmental monitoring program carried out in the same facility. Alcohol based biocides showed bactericidal and fungicidal activity but no sporicidal activity. Chlorine based compounds and glutaraldehyde showed bactericidal and fungicidal effect while, the sporicidal effects depended on the used dilution and the contact time. Hydrogen peroxide showed bactericidal, fungicidal and sporicidal activity. Quaternary ammonium compounds tested showed very weak activity in all tests. Evaluation of the biocide is an essential step to guarantee the use of the most appropriate agent in any location.
“…Ancak son zamanlardaki çalışmalarda, dezenfektanların etkinliği ve bu konuda kullanıma giren ticari kitler konusu ön plana çıkmıştır (18). Esasen dezenfektanların etkinliğinin belirlenmesi için standardardize edilmiş ulusal ve uluslararası rehbehler bulunmaktadır (19,20). Sodyum hipoklorit, moleküler laboratuvarları için kullanışlı bir dezenfektan olup, hazırlaması kolay ve ucuz olduğu için yaygın olarak kullanılmaktadır (21,22).…”
“…In these tests, stainless steel, glass or plastic surfaces are used as a model surface (Hayes, 1992;Frank and Chmielewski, 1997;Andrade et al, 1998;Klingeren et al, 1998;Grönholm et al, 1999;Rossoni andGaylerde, 2000, Cremieux et al, 2001). Agar diffusion method is generally used for determination of antimicrobial activity of chemotherapeutic agents but it can be also used for evaluating the disinfectant efficacy (Seeley and VanDemark, 1972;Kovacs et al, 1998;Alexander and Strete, 2001).…”
In this study, efficacy of seven different commercial disinfectant preparations was investigated against characteristic bacteria of a poultry slaughterhouse in Ankara (Turkey) by using paper disc-agar diffusion method and surface effectiveness test. According to paper disc-agar diffusion method, some disinfectants had wide efficacy against the test bacteria. The disinfectant effectiveness generally increased by the increasing of disinfectant concentration. However, some disinfectants were ineffective even though at their highest concentrations. The most effective disinfectant was B which contains QAC as active agent. Staphylococcus spp., Staphylococcus aureus and Escherichia coli were the most sensitive bacteria to the disinfectants. Some pathogenic isolates, especially Salmonella spp. and Campylobacter spp., were the most resistant ones to many of the disinfectants tested. The results of paper disc-agar diffusion method indicated the importance of characteristic bacterial strains of food plants as the test bacteria for disinfectant efficacy tests. Conversely of paper disc-agar diffusion method, all disinfectants were effective against the isolates by surface effectiveness test depending on exposure time. The disinfectants, except A and F, produced at least 3 log unit reduction during 5 min of exposure. However, all disinfectants at their lowest concentrations were effective against all tested bacteria during 15 and 30 min by this test.
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