Assessment of the efficacy of some physico-chemical and hormonal treatments for dormancy removal &amp; germination improvement in Bunium persicum (Kala Zeera)

Banyal, Shelly; Sharma, Sandeep

doi:10.22271/chemi.2020.v8.i2ai.9097

Cited by 6 publications

(4 citation statements)

References 25 publications

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“…Since GA 3 and cold stratification increased seed germination, the seeds also have PD (Figures 4 and 5), i.e., they have MPD. Our data are in parallel with the previous results of Sharifi and Pouresmael (2006) and Banyal and Sharma (2020), who reported MPD for B. persicum seeds, but different from those of Sharma and Sharma (2010) and Saeidnejad et al (2013), who reported only PD. However, none of these four studies provided any information on the embryo growth prior to germination, and hence the seed dormancy class was only determined based on seed germination responses to cold stratification and GA 3 treatment.…”

Section: Discussionsupporting

confidence: 91%

“…Bonyanpour and Khosh‐khui (2001), Sharifi and Pouresmael (2006), Sasani et al (2007), Sharma and Sharma (2010), and Saeidnejad et al (2013) have reported that seed dormancy of B. persicum can be broken only by cold stratification; however, gibberellic acid (GA) alone breaks seed dormancy and increases germination rates (Amooaghaie & Ahmadi, 2017; Bahadori & Javanbakht, 2006; Emamipoor & Maziah, 2014; Kamali & Sadeghipour, 2016). In a previous study, germination of B. persicum seeds collected from India was promoted by GA, indole acetic acid, and kinetin at 4°C but not at 25°C; seed deterioration occurred at 25°C (Banyal & Sharma, 2020).…”

Section: Introductionmentioning

confidence: 95%

“…Sasani et al (2007) reported that after‐ripening (dry storage) of B. persicum seeds for 3 years broke seed dormancy and improved germination rates, but Sharma and Sharma (2010) observed that after‐ripening reduced the seed quality of B. persicum due to deterioration. Uncertainties and ambiguities in defining the class of seed dormancy in B. persicum have resulted in PD, MPD, deep PD, and deep complex MPD being assigned to the seeds of this species (Banyal & Sharma, 2020; Emamipoor & Maziah, 2014; Saeidnejad et al, 2013; Sharifi & Pouresmael, 2006; Sharma & Sharma, 2010). However, none of the reports provided any information on embryo growth, which is required in determining the class of seed dormancy.…”

Section: Introductionmentioning

confidence: 99%

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Variation in degree of intermediate complex morphophysiological dormancy in seeds of Bunium persicum (Apiaceae)

Mohammadianfar,

Ghaderi‐Far,

Torabi

et al. 2023

Plant Species Biology

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Bunium persicum is an important medicinal plant, but the requirements for seed dormancy break and germination are not well known. Based on available information, we hypothesized that seeds have an underdeveloped embryo and physiological dormancy (PD), and hence morphophysiological dormancy (MPD), and that populations of this species may have different degrees of MPD which demand different germination requirements. In this study, we evaluated embryo growth in seeds from four B. persicum populations at different temperatures and tested germination at varying constant temperatures, with or without GA3 and cold stratification. Seeds from the four populations had a fully differentiated, small, linear but underdeveloped embryo. The initial embryo‐to‐seed length ratio was 0.14–0.17, but it increased to 0.71–0.75 before germination at 5°C. Little embryo growth occurred at 15°C and no growth occurred at 25°C. GA3 effectively increased germination percentages, replaced cold stratification, reduced the required period for cold stratification, and in combination with cold stratification increased the maximum temperature of germination. Therefore, seeds from the four populations have intermediate complex MPD. Additionally, the germination responses of seeds from the four populations to GA3, cold stratification, or both were different, suggesting variation in the degree of PD. Bunium persicum seeds developed in cool and wet conditions were more dormant than those developed under warm and dry conditions. Thus, we showed that different climates can affect the depth of PD in a species whose seeds have MPD, resulting in variation in the degree of intermediate complex MPD at the population level.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

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Variation in degree of intermediate complex morphophysiological dormancy in seeds of Bunium persicum (Apiaceae)

Mohammadianfar,

Ghaderi‐Far,

Torabi

et al. 2023

Plant Species Biology

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“…Three to four-year juvenile period is required to develop into Black Zira tuber and yield. The long life cycle of this plant prevents commercial cultivation of this plant (Banyal and Sharma 2020). Thus, the plant is critically endangered in natural habitats due to genetic erosion, pasture degradation, and early harvest.…”

Section: Introductionmentioning

confidence: 99%

Dormancy breaking, indirect somatic embryogenesis, and encapsulation of somatic embryos in Black Zira

Ouzhand,

Mohayeji,

Pourseyedi

et al. 2023

Plant Cell Tiss Organ Cult

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Black Zira (Bunium persicum) is an important endemic medicinal plant in Iran. This research was conducted out to assay breaking seed dormancy, develop new protocols for callus induction, somatic embryogenesis, and arti cial seed. Seeds were treated with ultrasonic waves in different periods (0, 1, 3, 5, 7, and 9 min). Then, two experiments with IBA (0, 1, 2, 3, and 4 mg L − 1 ) and a combination of different levels of 2,4-D (0, 1, 2, and 4 mg/l) and BAP (0, 0.5, 1, and 2 mg/l) were designed. Different concentrations of sodium alginate (2, 3, and 4%) and CaCl 2 (50, 75, and 100 mM) were prepared for arti cial seed production. According to the results, the 7 min ultrasonic waves had the maximum effect on germination (81.87%), while the highest negative effect on germination (2.5%) was reported from 9 min exposure time. In the IBA experiment, the highest percentages of callogenesis, callus weight, and adventitious root were obtained from MS medium containing 4 mg/l IBA. Moreover, 1 mg/l IBA resulted in the highest embryogenesis percentage. In the second experiment, the highest percentage of callus formation and callus weight were achieved in 1 mg/l BAP + 4 mg/l 2,4-D, 0.5 mg/l BAP + 4 mg/l 2,4-D, and 2 mg/l 2,4-D media. The maximum embryogenesis percentage was seen in 0.5 mg/l BAP + 1 mg/l 2,4-D, 0.5 mg/l BAP + 2mg/l 2,4-D, and 1 mg/l 2,4-D media. The highest germination rate (77.77%) was obtained from encapsulated embryos with 3% sodium alginate and 100 mM CaCl 2 . Key MessageA protocol with improved results has been developed for indirect somatic embryogenesis and, arti cial seed production in B.persicum using hypocotyl explants.

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Synthesis, biological investigation and catalytic application using the alcoholic extract of Black Cumin (Bunium Persicum) seeds-based silver nanoparticles

et al. 2021

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Assessment of the efficacy of some physico-chemical and hormonal treatments for dormancy removal & germination improvement in Bunium persicum (Kala Zeera)

Cited by 6 publications

References 25 publications

Variation in degree of intermediate complex morphophysiological dormancy in seeds of Bunium persicum (Apiaceae)

Variation in degree of intermediate complex morphophysiological dormancy in seeds of Bunium persicum (Apiaceae)

Dormancy breaking, indirect somatic embryogenesis, and encapsulation of somatic embryos in Black Zira

Synthesis, biological investigation and catalytic application using the alcoholic extract of Black Cumin (Bunium Persicum) seeds-based silver nanoparticles

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