Assessment of the Efficacy of Platelet-Rich Plasma in the Treatment of Traumatic Canine Fractures

López, Sergio; Vilar, Jose M. G.; Sopena, Joaquín; Damia, E; Chicharro, Déborah; Carrillo, José M.; Cuervo, Belén; Rubio, Mónica

doi:10.3390/ijms20051075

Cited by 20 publications

(21 citation statements)

References 24 publications

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“…Intra-articular injection of PRP also prevented cytotoxic effect of lidocaine on chondrocytes [48] Thus, intra-articular injections of PRP can be useful in angiogenesis and extracellular matrix remodeling, cell proliferation, and differentiation for osteoarthritis [12,13,15,16]. Applications of PRP in fracture repair had been shown in both dogs [49] and rabbit [48] by promoting bone healing. Interestingly, injection of PRP also promoted wound healing of soft tissue defects [50,51] as well as periodontal regeneration [52].…”

Section: Discussionmentioning

confidence: 98%

Optimization of a rapid one-step platelet-rich plasma preparation method using syringe centrifugation with and without carprofen

et al. 2020

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Background: Carprofen and platelet-rich plasma (PRP) are widely used in small animal clinical practice. Separation layers have been used during blood centrifugation to increase platelet yield. The objectives of this study were to (1) identify the optimal centrifugation force for the one-step PRP preparation, (2) determine whether there is an advantage to using carprofen in one-step PRP preparation, and (3) compare platelet morphology from one-step PRP preparation with and without carprofen. We hypothesized that injectable carprofen (emulsion formula) could be used successfully as the separation layer in PRP preparation. Results: Samples from 14 healthy dogs were used to determine the optimal centrifugation force using one-step PRP preparation in a disposable syringe without carprofen, with forces set at 300, 500, 700, 900, 1100, 1300, and 1500 xg for 5 min. Optimum centrifugation force, plasma volume, and platelet concentrations of one-step PRP preparation were found and recovered at 900 xg, 1.9 ± 0.28 ml, and 260.50 ± 58.39 X 10 3 cell/μl, respectively. Samples from 12 healthy dogs were used to determine the optimal force (with forces set at 300, 500, 700, and 900 xg) for 5 min using one-step PRP preparation with carprofen. Optimum centrifugation force, plasma volume, and platelet concentrations for one-step PRP preparation with carprofen were found and recovered at 500 xg, 0.62 ± 0.16 ml and 948.50 ± 261.40 X 10 3 cell/μl, respectively. One-step PRP preparation with carprofen increased the platelet yield from baseline by 1.76 and 4.95 fold, respectively. Samples from 3 healthy dogs were used to observe platelet morphologies after centrifugation by scanning electron microscopy. Images of platelets on glass slides from both preparation methods revealed pseudopods emerging from the margins of the discoid platelets. Conclusions: One-step PRP centrifugation both with and without carprofen increased the platelet yield, but using carprofen (emulsion formula) as a separation layer resulted in a higher platelet yield. The clinical usefulness of PRP products from these methods should be further investigated.

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Section: Discussionmentioning

confidence: 98%

Optimization of a rapid one-step platelet-rich plasma preparation method using syringe centrifugation with and without carprofen

et al. 2020

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“…Nine in vivo preclinical studies (55)(56)(57)(58)(59)(60)(61)(62)(63) enrolled in this systematic review investigated the effect of PRP in the animal fracture model. The risk of bias of in vivo preclinical studies in this study was independently evaluated by two reviewers.…”

Section: In Vivo Preclinical Studiesmentioning

confidence: 99%

Platelet-Rich Plasma for Bone Fracture Treatment: A Systematic Review of Current Evidence in Preclinical and Clinical Studies

et al. 2021

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Background: Recently, there is an increasing interest in the therapeutic potential of platelet-rich plasma (PRP) for bone fracture treatment. Nevertheless, the effect of PRP for bone fracture treatment remains controversial and is still a matter of discussion. Therefore, we performed a systematic review to evaluate the efficacy and safety of PRP injection for treatment of bone fracture.Methods: The main bibliographic databases, including Medline, PubMed, Embase, Web of Science, and the Cochrane library, were comprehensively searched for studies focusing on the application of platelet-rich plasma (PRP) on bone fracture treatment. All relevant articles were screened for eligibility and subdivided into the preclinical and clinical studies. Data were extracted and presented systematically.Results: Finally, twenty-six in vitro preclinical studies (basic studies), nine in vivo preclinical studies (animal studies), and nine clinical studies, met the selection criteria, and were included in the present systematic review. Preclinical studies showed an overall positive effect of PRP on osteoblast-like cells in vitro and bone healing in animal models. The most used treatment for bone fracture in animal and clinical studies is fixation surgery combined with PRP injection. The clinical studies reported PRP shortened bony healing duration, and had no positive effect on improving the healing rate of closed fractures. However, the results of functional outcomes are controversial. Additionally, compared with control group, PRP would not increase the rate of postoperative wound infection.Conclusion: The present systematic review confirmed the continuing interests of PRP as an additional treatment for bone fracture. Preclinical studies highlighted the potential value of PRP as promising therapy for bone fracture. However, the preclinical evidence did not translate into a similar result in the clinical studies. In addition, types of fractures and procedures of PRP preparation are heterogeneous in enrolled studies, which might result in controversial results. Meanwhile, characteristics of PRP, such as platelet concentration, the numbers of leukocytes, still need to be determined and further research is required.

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“…Clinical and radiological follow-up was done at 4 weeks, 8 weeks, and 18 months ( Figure 3 ). Each radiograph was evaluated by a stage score from 1 to 5 points (1, not visible callus formation; 2, barely visible callus formation; 3, scattered, not homogeneous calluses; 4, uniform, mature callus formation; 5, very active, hypertrophic callus formation) ( 17 ). Bone formation was also quantified morphometrically with Adobe Photoshop CS6 (Adobe, San Jose, CA, USA) and OLYMPUS CellSens Dimension 1.15 (Olympus Corporation, Japan) ( Figure 4 ).…”

Section: Case Reportmentioning

confidence: 99%

Case Report: First Evidence of a Benign Bone Cyst in an Adult Teckel Dog Treated With Shark Teeth-Derived Bioapatites

García-González

Muñoz

González‐Cantalapiedra

et al. 2021

Front. Vet. Sci.

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Bone cysts are a very rare orthopedic pathology in veterinary medicine, the general prevalence of which is unknown. A unicameral bone cyst was diagnosed in an adult female Teckel dog with a limp that was treated surgically by filling the defect with marine bioapatites. The treatment was effective and at 8 weeks the defect had remodeled 50.24%. Eighteen months after surgery, the defect had remodeled 94.23%. The limp disappeared after surgery, and functional recovery was good in all stages after surgery. No adverse reactions were observed at the local or systemic level. This is the first report of a benign bone cyst in an lame adult female Teckel successfully treated with a novel marine bioapatite.

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Assessment of the Efficacy of Platelet-Rich Plasma in the Treatment of Traumatic Canine Fractures

Cited by 20 publications

References 24 publications

Optimization of a rapid one-step platelet-rich plasma preparation method using syringe centrifugation with and without carprofen

Optimization of a rapid one-step platelet-rich plasma preparation method using syringe centrifugation with and without carprofen

Platelet-Rich Plasma for Bone Fracture Treatment: A Systematic Review of Current Evidence in Preclinical and Clinical Studies

Case Report: First Evidence of a Benign Bone Cyst in an Adult Teckel Dog Treated With Shark Teeth-Derived Bioapatites

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