Assessment of the effects of Cu and Ag in oysters Crassostrea gigas (Thunberg, 1793) using a battery of cell and tissue level biomarkers

Rementerı́a, Aitor; Mikolaczyk, Mathilde; Lanceleur, Laurent; Blanc, Guillaume; Soto, Manu; Schäfer, Jörg; Zaldibar, Beñat

doi:10.1016/j.marenvres.2016.09.002

Cited by 22 publications

(14 citation statements)

References 48 publications

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“…At least the first of these parameters—the CDT ratio—shows an obvious degradation in dependence of Cd exposure in both the CdL and CdH-treated winkles ( Figure 10 A). Similar results were reported from other studies in different mollusc species exposed to environmental stressors [ 79 , 80 ].…”

Section: Discussionsupporting

confidence: 92%

Successive Onset of Molecular, Cellular and Tissue-Specific Responses in Midgut Gland of Littorina littorea Exposed to Sub-Lethal Cadmium Concentrations

Benito

Niederwanger

Izagirre

et al. 2017

IJMS

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Cadmium (Cd) is one of the most harmful metals, being toxic to most animal species, including marine invertebrates. Among marine gastropods, the periwinkle (Littorina littorea) in particular can accumulate high amounts of Cd in its midgut gland. In this organ, the metal can elicit extensive cytological and tissue-specific alterations that may reach, depending on the intensity of Cd exposure, from reversible lesions to pathological cellular disruptions. At the same time, Littorina littorea expresses a Cd-specific metallothionein (MT) that, due to its molecular features, expectedly exerts a protective function against the adverse intracellular effects of this metal. The aim of the present study was, therefore, to assess the time course of MT induction in the periwinkle’s midgut gland on the one hand, and cellular and tissue-specific alterations in the digestive organ complex (midgut gland and digestive tract) on the other, upon exposure to sub-lethal Cd concentrations (0.25 and 1 mg Cd/L) over 21 days. Depending on the Cd concentrations applied, the beginning of alterations of the assessed parameters followed distinct concentration-dependent and time-dependent patterns, where the timeframe for the onset of the different response reactions became narrower at higher Cd concentrations compared to lower exposure concentrations.

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Section: Discussionsupporting

confidence: 92%

Successive Onset of Molecular, Cellular and Tissue-Specific Responses in Midgut Gland of Littorina littorea Exposed to Sub-Lethal Cadmium Concentrations

Benito

Niederwanger

Izagirre

et al. 2017

IJMS

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“…Paraffin sections (5 μm) were stained with haematoxylin-eosin (H/ E) in order to analyze the integrity of the digestive gland, gonad and gills. Vv BAS is a general stress biomarker that measures the relative increase of basophilic cells under stress conditions in the digestive alveoli of molluscs (Zorita et al, 2006;Rementeria et al, 2016;Benito et al, 2017). A high CTD ratio indicates a loss of the integrity of the digestive gland of molluscs, and it can be caused by a chemical stressor or by an insufficient nutritive status (Mújica et al, 2015b;Benito et al, 2017).…”

Section: Tissue-level Biomarkers and Histopathologymentioning

confidence: 99%

“…Environmental health assessments cannot be performed solely based on chemical analysis as it does not provide information on adverse effects exerted by contaminants on living organisms. Therefore, the measurement of pollutantinduced biological effects has become of major importance for these assessments (Cajaraville et al, 2000;Rementeria et al, 2016;Benito et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Influence of season-depending ecological variables on biomarker baseline levels in mussels (Mytilus trossulus) from two Baltic Sea subregions

Benito

Ahvo

Nuutinen

et al. 2019

Science of The Total Environment

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“…The connective tissue surrounding the digestive tubules of oysters is known to play 92 an important role as energy reservoir (Thompson et al, 1996). The depletion and relocation of 93 neutral lipids has been described as an alteration in oysters exposed to heavy metals 94 (Rementeria et al, 2016;Séguin et al, 2016) and therefore this endpoint was investigated as an 95 effect biomarker. Other endpoints include lipofuscins which are one of the main cellular 96 products accumulating in lysosomes as a result of enhanced oxidative damage.…”

Section: Introduction 38mentioning

confidence: 99%

“…Other endpoints include lipofuscins which are one of the main cellular 96 products accumulating in lysosomes as a result of enhanced oxidative damage. This increased 97 oxidative damage has been previously reported in mollusks exposed to metals (Marigómez et 98 al., 2013;Rementeria et al, 2016). The presence of metals and other stress situations have been 99…”

Section: Introduction 38mentioning

confidence: 99%

Organotropism and biomarker response in oyster Crassostrea gigas exposed to platinum in seawater

Abdou

Zaldibar

Medrano

et al. 2018

Environ Sci Pollut Res

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Platinum (Pt) is a Technology Critical Element (TCE) for which biogeochemical cycles are still 13 poorly understood. This lack of knowledge includes Pt effects on marine organisms, which 14 proved to be able to bioconcentrate this trace element. Oysters Crassostrea gigas were exposed 15 to stable Pt isotope spiked daily in seawater for 35 days. Seawater was renewed daily and spiked 16 (with Pt(IV)) to three nominal Pt concentrations (50, 100, and 10,000 ng.L −1 ) for two replicate 17 series. Organotropism study revealed that gills, and to a lesser extent mantle, are the key organs 18 regarding Pt accumulation, although a time-and concentration-dependent linear increase in Pt 19 levels occurred in all the organs investigated (i.e. digestive gland, gonads, gills, mantle, and 20 muscle). In oysters exposed to Pt concentrations of 10,000 ng.L -1 , significant biomarker 21 impairments occurred, especially at cellular levels. They reflect altered lipofuscin and neutral 22 lipid contents, as well as intralysosomal metal accumulation. These observations were 23 attributed to activation of excretion / detoxification mechanisms, including Pt elimination 24 through faeces and clearly support the importance of the digestive gland in the response to 25 direct Pt exposure. Despite relatively constant condition index, the Integrative Biological 26Response Index (IBR) suggests a generally decreasing health status of oysters. 27

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Assessment of the effects of Cu and Ag in oysters Crassostrea gigas (Thunberg, 1793) using a battery of cell and tissue level biomarkers

Cited by 22 publications

References 48 publications

Successive Onset of Molecular, Cellular and Tissue-Specific Responses in Midgut Gland of Littorina littorea Exposed to Sub-Lethal Cadmium Concentrations

Successive Onset of Molecular, Cellular and Tissue-Specific Responses in Midgut Gland of Littorina littorea Exposed to Sub-Lethal Cadmium Concentrations

Influence of season-depending ecological variables on biomarker baseline levels in mussels (Mytilus trossulus) from two Baltic Sea subregions

Organotropism and biomarker response in oyster Crassostrea gigas exposed to platinum in seawater

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