Assessment of the effectiveness of cyclosporine nasal spray in an animal model of allergic rhinitis

Abstract

The aim of this study is to show if cyclosporine has an antiallergic role in a rat model of ovalbumin-induced allergic rhinitis. The 54 rats were divided into six equal groups. The first group was a negative control group without induced allergic rhinitis; the second group a positive control with induced allergic rhinitis not receiving treatment. The remaining four groups, after induction of allergic rhinitis, received intranasal cyclosporine treatment in doses of 0.05, 0.1, or 0.2% or nasal steroid treatment. In the biochemical examination, on the surface of the tissue tumor necrosis factor (TNF) interferon (IFN), interleukin (IL)-5, IL-13, as well as IL-2, IL-4, IL-17A, and IgE were studied. Histologically, ciliary loss, increase of goblet cells, vascular congestion, and the degree of eosinophil infiltration were rated. In all treatment groups, on average, a significant reduction in all histological and biochemical values was found compared to the positive control group. Comparing each of the three cyclosporine-using groups with the group of nasal corticosteroid did not show any significant difference in the average scores. Cyclosporine nasal drops are effective to be used in an animal model of experimental allergic rhinitis without systemic effects.

Astragalus Polysaccharides/Chitosan Microspheres for Nasal Delivery: Preparation, Optimization, Characterization, and Pharmacodynamics

“…According to some studies (Senturk et al, 2018), cyclosporine nasal sprays have been used to treat AR in an animal model (54 healthy female Sprague-Dawley rats). In our study, APS/CTS microspheres were designed for nasal delivery in a total of 60 healthy Wistar rats.…”

“…In our study, APS/CTS microspheres were designed for nasal delivery in a total of 60 healthy Wistar rats. In the biochemical examination, Erol Senturk et al (2018) studied the surface of the tissue tumor necrosis factor (TNF), interferon (IFN), interleukin (IL)-5, IL-13, IL-2, IL-4, IL-17A, and IgE. In a future experiment, we will further explore these responses and their application in human AR.…”

“…The rats were observed for sneezing, scratching, and rhinorrhea and scored within 30 min after each challenge or administration. The allergic rat model was considered to be successful when symptom scores were higher than 5 (Senturk et al, 2018). Score 1: the time of sneezing was 1-3 or scratching was 1-5 or reaching the anterior nostril; Score 2: the time of sneezing was 4-10 or scratching was 6-15 or over the anterior nostril; and Score 3: the time of sneezing was more than 10 or scratching was more than 15 or flowing all the face.…”

Effects of a Thermosensitive In Situ Gel Containing Mometasone Furoate on a Rat Allergic Rhinitis Model

“…[18][19][20][21][22] In our study, antigen-induced nasal symptoms were remarkably increased in the AR model group by OVA administration (Figures 2 and 3) being in accordance with the previous reports. 18,[23][24][25] Therefore, it was considered that the AR model was successfully developed in this study.…”

Petroleum extract of Farfarae Flos alleviates nasal symptoms by regulating the Th1-Th2 cytokine balance in a mouse model of Allergic Rhinitis

“…As previously described [21], the nasal symptom score was based on three features, each graded on a four-point scale score between 0 and 3: (i) sneezing: 0, none; 1, 1-3 sneezes per 20 min; 2, 4-10 sneezes per 20 min; 3, ≥11 sneezes per 20 min; (ii) nasal mucus: 0, none; 1, mucus in nostrils; 2, mucus outflow from nostrils; 3, mucus outflow to face; and (iii) nasal itching: 0, none; 1, nose rubbing 1-2 times per min; 2, nose rubbing 3-5 times per min; 3, nose rubbing >6 times per min. The three scores were added to give a total score between 0 and 9 points.…”