Assessment of the effect of grape seed cake inclusion in the diet of healthy fattening‐finishing pigs

Ţăranu, Ionelia; Hăbeanu, Mihaela; Gras, Mihail Alexandru; Pistol, Gina Cecilia; Lefter, Nicoleta Aurelia; Palade, Laurentiu Mihai; Ropotă, Mariana; Chedea, Veronica Sanda

doi:10.1111/jpn.12697

Cited by 33 publications

(30 citation statements)

References 67 publications

(89 reference statements)

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“…GP diet increased TAS in both the colon and duodenum of piglets and this result is better than those of antioxidant capacity testing in the plasma of piglets fed grape seed and grape mark diet [ 55 ] or with plant extracts including grape seeds [ 31 ]. Also, Taranu et al (2018) registered no different TSA in comparison with the control in the liver of fattening pigs fed grape seed diet [ 56 ].…”

Section: Discussionmentioning

confidence: 99%

“…TBARS-MDA is the main by-product of lipid peroxidation, and previous studies reported that the antioxidant compounds (flavonoids, phenols, PUFA) from grape extracts or grape by-products are able to control and diminish the TBARS level by their ability to scavenge free radicals [ 56 , 57 , 58 ].…”

Section: Discussionmentioning

confidence: 99%

“…Many studies provided evidence about the reducing effect of dietary polyphenols on TBARS-MDA in plasma or liver but very few on duodenum or colon. For example, administration of grape seed extract inhibited MDA formation in the liver tissues of ICR/f rat and in the body and liver of rabbit [ 56 , 59 ]. In weaned piglets, the study of Zhang et al (2014) showed that supplementation of the diet with a combination of plant extracts including grape seed (2 g/kg) was effective in reducing TBARS formation in the plasma [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

“…In weaned piglets, the study of Zhang et al (2014) showed that supplementation of the diet with a combination of plant extracts including grape seed (2 g/kg) was effective in reducing TBARS formation in the plasma [ 31 ]. Grape seed and grape marc did not influence TBARS concentration in plasma and liver of piglets [ 55 ] and in the liver of fattening pigs fed grape seed diet [ 56 ].…”

Section: Discussionmentioning

confidence: 99%

“…In order to cope with the high exposure to oxidative stress, the gut mucosa, like other tissues, possesses several ‘lines of defense’ against ROS [ 60 ]. These include repair mechanisms, prevention of the ROS production cascade (e.g., by antioxidant bioactive compounds like polyphenols) and the antioxidants defense system including the antioxidant enzymes, such as SOD, GPx and CAT, the main parameters involved in the cellular defense against free radicals [ 56 , 60 ]. Herein, independently of the diet, duodenum exhibited a higher level of CAT and GPx activity compared to colon.…”

Section: Discussionmentioning

confidence: 99%

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Intestinal Absorption and Antioxidant Activity of Grape Pomace Polyphenols

et al. 2018

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The absorption and antioxidant activity of polyphenols from grape pomace (GP) are important aspects of its valorization as a feed additive in the diet of weaned piglets. This study aimed to evaluate the presence of polyphenols from GP both in vitro in IPEC cells and in vivo in the duodenum and colon of piglets fed with diets containing or not 5% GP and also to compare and correlate the aspects of their in vitro and in vivo absorption. Total polyphenolic content (TPC) and antioxidant status (TAS, CAT, SOD and GPx enzyme activity, and lipid peroxidation-TBARS level) were assessed in duodenum and colon of piglets fed or not a diet with 5% GP. The results of UV-Vis spectroscopy demonstrated that in cellular and extracellular medium the GP polyphenols were oxidized (between λmax = 276 nm and λmax = 627.0 nm) with the formation of o-quinones and dimers. LC-MS analysis indicated a procyanidin trimer possibly C2, and a procyanidin dimer as the major polyphenols identified in GP, 12.8% of the procyanidin trimer and 23% of the procyanidin dimer respectively being also found in the compound feed. Procyanidin trimer C2 is the compound accumulated in duodenum, 73% of it being found in the colon of control piglets, and 62.5% in the colon of GP piglets. Correlations exist between the in vitro and in vivo investigations regarding the qualitative evaluation of GP polyphenols in the cells (λmax at 287.1 nm) and in the gut (λmax at 287.5 nm), as oxidated metabolic products. Beside the presence of polyphenols metabolites this study shows also the presence of the unmetabolized procyanidin trimers in duodenum and colon tissue, an important point in evaluating the benefic actions of these molecules at intestinal level. Moreover the in vivo study shows that a 5% GP in piglet’s diet increased the total antioxidant status (TAS) and decreased lipid peroxidantion (TBARS) in both duodenum and colon, and increased SOD activity in duodenum and CAT and GPx activity in colon. These parameters are modulated by the different polyphenols absorbed, mainly by the procyanidin trimers and catechin on one side and the polyphenols metabolites on the other side.

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Section: Discussionmentioning

confidence: 99%