Assessment of the effect of Schistosoma haematobium co infection on malaria parasites and immune responses in rural populations in Gabon: study protocol

Ngoa, Ulysse Ateba; Zinsou, Jeannot Fréjus; Kassa, Roland Fabrice Kassa; Feugap, Eliane Ngoune; Honkpéhèdji, Yabo Josiane; Loembé, Marguerite Massinga; Moundounga, Hilaire Kenguele; Mouima, Anne Marie Nkoma; Mbenkep, Lima Honorine; Wammes, Linda J.; Mbow, Moustapha; Kruize, Yvonne C. M.; Mombo-Ngoma, Ghyslain; Hounkpatin, Aurore Larissa Bouyoukou; Dejon-Agobé, Jean Claude; Issifou, Saadou; Lell, Bertrand; Smits, Hermelijn H.; Kremsner, Peter G.; Yazdanbakhsh, Maria; Adegnika, Ayôla Akim

doi:10.1186/2193-1801-3-388

Cited by 19 publications

(26 citation statements)

References 41 publications

(58 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One of the major limitations of our study concerns the absence of data with respect to co-infections such as, for example, chronic maternal helminth infections. Such infections are known to affect anti-plasmodial immune responses [ 75 – 77 ]. Furthermore, contact in utero with maternally-derived filariasis antigens reportedly modifies cord blood cell responses such that they display a predominantly anti-inflammatory profile [ 78 ].…”

Section: Discussionmentioning

confidence: 99%

Infants' Peripheral Blood Lymphocyte Composition Reflects Both Maternal and Post-Natal Infection with Plasmodium falciparum

et al. 2015

View full text Add to dashboard Cite

Maternal parasitoses modulate fetal immune development, manifesting as altered cellular immunological activity in cord blood that may be linked to enhanced susceptibility to infections in early life. Plasmodium falciparum typifies such infections, with distinct placental infection-related changes in cord blood exemplified by expanded populations of parasite antigen-specific regulatory T cells. Here we addressed whether such early-onset cellular immunological alterations persist through infancy. Specifically, in order to assess the potential impacts of P. falciparum infections either during pregnancy or during infancy, we quantified lymphocyte subsets in cord blood and in infants' peripheral blood during the first year of life. The principal age-related changes observed, independent of infection status, concerned decreases in the frequencies of CD4+, NKdim and NKT cells, whilst CD8+, Treg and Teff cells' frequencies increased from birth to 12 months of age. P. falciparum infections present at delivery, but not those earlier in gestation, were associated with increased frequencies of Treg and CD8+ T cells but fewer CD4+ and NKT cells during infancy, thus accentuating the observed age-related patterns. Overall, P. falciparum infections arising during infancy were associated with a reversal of the trends associated with maternal infection i.e. with more CD4+ cells, with fewer Treg and CD8+ cells. We conclude that maternal P. falciparum infection at delivery has significant and, in some cases, year-long effects on the composition of infants' peripheral blood lymphocyte populations. Those effects are superimposed on separate and independent age- as well as infant infection-related alterations that, respectively, either match or run counter to them.

show abstract

Section: Discussionmentioning

confidence: 99%

Infants' Peripheral Blood Lymphocyte Composition Reflects Both Maternal and Post-Natal Infection with Plasmodium falciparum

et al. 2015

View full text Add to dashboard Cite

show abstract

“…haematobium and P . falciparum are the main prevalent species of schistosomiasis and malaria [ 13 , 14 ]. We thus assessed the effect of S .…”

Section: Introductionmentioning

confidence: 99%

Schistosoma haematobium effects on Plasmodium falciparum infection modified by soil-transmitted helminths in school-age children living in rural areas of Gabon

et al. 2018

Self Cite

View full text Add to dashboard Cite

BackgroundMalaria burden remains high in the sub-Saharan region where helminths are prevalent and where children are often infected with both types of parasites. Although the effect of helminths on malaria infection is evident, the impact of these co-infections is not clearly elucidated yet and the scarce findings are conflicting. In this study, we investigated the effect of schistosomiasis, considering soil-transmitted helminths (STH), on prevalence and incidence of Plasmodium falciparum infection.MethodologyThis longitudinal survey was conducted in school-age children living in two rural communities in the vicinity of Lambaréné, Gabon. Thick blood smear light microscopy, urine filtration and the Kato-Katz technique were performed to detect malaria parasites, S. haematobium eggs and, STH eggs, respectively. P. falciparum carriage was assessed at inclusion, and incidence of malaria and time to the first malaria event were recorded in correlation with Schistosoma carriage status. Stratified multivariate analysis using generalized linear model was used to assess the risk of plasmodium infection considering interaction with STH, and survival analysis to assess time to malaria.Main findingsThe overall prevalence on subject enrolment was 30%, 23% and 9% for S. haematobium, P. falciparum infections and co-infection with both parasites, respectively. Our results showed that schistosomiasis in children tends to increase the risk of plasmodium infection but a combined effect with Trichuris trichiura or hookworm infection clearly increase the risk (aOR = 3.9 [95%CI: 1.7–9.2]). The incidence of malaria over time was 0.51[95%CI: 0.45–0.57] per person-year and was higher in the Schistosoma-infected group compared to the non-infected group (0.61 vs 0.43, p = 0.02), with a significant delay of time-to first-malaria event only in children aged from 6 to 10-years-old infected with Schistosoma haematobium.ConclusionsOur results suggest that STH enhance the risk for P. falciparum infection in schistosomiasis-positive children, and when infected, that schistosomiasis enhances susceptibility to developing malaria in young children but not in older children.

show abstract

“…Urine and blood samples were collected at inclusion for the diagnosis of S. haematobium and P. falciparum infection as well as for immunological assays. A thorough description of the parasitological test and the immunological assays has been previously published in a study protocol article [ 31 ]. Briefly, S. haematobium infection was determined by the detection of eggs by microscopy in 10 ml of filtrated urine.…”

Section: Methodsmentioning

confidence: 99%

“…Peripheral blood was collected in sodium heparinized tubes for every child as described elsewhere [ 31 ]. To assess the innate immune response heparinised blood was diluted in RPMI (1:1) and cultured for 24 hours at 37°C with a panel of five different Toll like receptor ligands (LPS [TLR4], PAM3 [TLR1/2], CPG [TLR9] CL097 [TLR7/8]), S. haematobium eggs antigens (SEA) and a combination of LPS and SEA.…”

Section: Methodsmentioning

confidence: 99%

“…In order to assess the adaptive immune response, PBMC were isolated by density gradient centrifugation on Ficoll as already described [ 31 ]. PBMCs were cultured for 72 hours at 37°C with SEB and supernatant was collected for the measurement of a panel of 11 different cytokines (TNF, IFNγ, IL-2, IL-4, IL-5, IL-13, IL-17A, IL-17 F, IL-22, IL-10, and IL-21) by a multiplex beads array immunoassay.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Cytokine and chemokine profile of the innate and adaptive immune response of schistosoma haematobium and plasmodium falciparum single and co-infected school-aged children from an endemic area of Lambaréné, Gabon

Ateba-Ngoa

Adegnika

Zinsou

et al. 2015

Malar J

Self Cite

View full text Add to dashboard Cite

BackgroundHelminths and malaria are among the most prevalent infectious diseases in the world. They both occur in tropical area where they often affect the same populations. There are studies suggesting an effect of helminths on malariometric indices. For example, malaria attacks as well as disease severity has been shown to be influenced by a concurrent chronic helminth infection. However, there are also studies that show no effect of concurrent helminth infections on malarial outcomes. To start addressing this issue, the effect of chronic Schistosoma haematobium infection on both the innate and adaptive immune response of Plasmodium falciparum-infected subjects was assessed in an area endemic for both these infections in Gabon.MethodSubjects infected with S. haematobium and or P. falciparum, as well as a control group with neither of these infections, were recruited. For innate immune response, heparinized blood was obtained and cultured for 24 hours with a panel of TLR ligands. For adaptive immune response, PBMC was isolated and stimulated with SEB for 72 hours. Cytokines and chemokines were measured in supernatants using a multiplex beads array immunoassay. Principal Component analysis was used to assess pattern of cytokine and chemokine responses representing the innate and adaptive components of the immune system.ResultsOverall it was observed that the presence of P. falciparum infection was marked by an increase in innate and adaptive immune responsiveness while S. haematobium infection was characterized by an increased chemokine profile, with at the same time, lower pro inflammatory markers. When the study subjects were split into single infected and co-infected groups no effect of S. haematobium on the immune response of P. falciparum infected subjects was observed, neither for the innate nor for the adaptive component of the immune response.ConclusionThis study provides original information on the cellular immune response of S. haematobium and/or P. falciparum in infected subjects. It rules out an effect of S. haematobium on the cytokine profile of subjects co-infected with P. falciparum.

show abstract

Assessment of the effect of Schistosoma haematobium co infection on malaria parasites and immune responses in rural populations in Gabon: study protocol

Cited by 19 publications

References 41 publications

Infants' Peripheral Blood Lymphocyte Composition Reflects Both Maternal and Post-Natal Infection with Plasmodium falciparum

Infants' Peripheral Blood Lymphocyte Composition Reflects Both Maternal and Post-Natal Infection with Plasmodium falciparum

Schistosoma haematobium effects on Plasmodium falciparum infection modified by soil-transmitted helminths in school-age children living in rural areas of Gabon

Cytokine and chemokine profile of the innate and adaptive immune response of schistosoma haematobium and plasmodium falciparum single and co-infected school-aged children from an endemic area of Lambaréné, Gabon

Contact Info

Product

Resources

About