Assessment of the Detrimental Impact of Polyvalent Streptophages Intended to be Used as Biological Control Agents on Beneficial Soil Streptoflora

Ashfield-Crook, Nina R.; Woodward, Zachary; Soust, Martin; Kurtböke, D. İpek

doi:10.1007/s00284-018-1565-2

Cited by 10 publications

(3 citation statements)

References 71 publications

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“…Therefore, bacteriophage treatments gained attention to minimise product losses and nutritional properties from disease causing bacteria. Like the previous successful treatments of potatoes [37][38][39] and strawberries, 40 the observed success of streptophage treatment on nuts in this study may indicate similar positive outcomes might be possible. Therefore, information generated via the studies like the one presented here can contribute toward development of effective phage biocontrol methods targeting different problems in agriculture.…”

Section: Discussionsupporting

confidence: 78%

Volume 43 Number 1

2022

Microbiol. Aust.

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Automation in diagnostic microbiology has provided accessibility to technology overcoming the delays inherent in culture-based technology and providing options to centralisation via use of digital imaging to interpret and report culture plates which enables cultures to be shared over the internet opening the virtual diagnostic microbiology laboratory. Automation is a continuous process which precipitates faster results and invites the diagnostic laboratory into a continuous processing, 24/7 world optimising what it has to offer for improved turnaround time (TAT) and improved workload management. While molecular technology was able to decrease TAT of results it was initially specialised and not freely available to the diagnostic microbiology laboratory becoming more a centralised subspeciality. The automation of molecular methods released the technology to be able to be decentralised and provide greater local access and community benefits from the technology and providing equity of community access to the technology. Thanks to automation, diagnostic microbiology is at the forefront of opening the availability of advances in testing to the broader community via decentralising technology and sharing technology in the virtual diagnostic microbiology laboratory.

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Section: Discussionsupporting

confidence: 78%

Volume 43 Number 1

2022

Microbiol. Aust.

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Section: Discussionsupporting

confidence: 78%

Detection and control of off-flavour compound-producing streptomycetes on locally produced nuts using streptophages

2022

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Members of the phylum Actinomycetota are the most prominent part of the soil microbiota, more specifically the species within the genus Streptomyces of this phylum. Key functions of Streptomyces species (or streptomycetes in general terms) include nutrient cycling and plant growth promotion and disease protection. However, these species can also produce volatile organic compounds, predominantly geosmin, which is responsible for musty and mildew scents that are unpleasant to humans and can negatively impact the nut crop industry as odorous nuts generally lose their market value. Bacterial viruses, called bacteriophages have been previously used successfully in agriculture and aquaculture to remove such odorous species and they may therefore be applied to the nut industry. To eliminate these compounds, the producer streptomycetes may be selectively removed from nut surfaces using streptophages. The removal of Streptomyces species from nut surfaces can then be expected to minimise geosmin production, therefore removing the unpleasant off-flavours and benefiting the nut industry.

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“…Because there is no known mechanism for effective disease suppression after initial infestation, multiple management strategies and approaches have been explored for the control of S. scabiei [22], including the effective use of virulent bacteriophages [25]. The novel tectiviruses reported in this work (Streptomyces phage WheeHeim and Streptomyces phage Forthebois) were isolated on S. scabiei and expand the existing collection of Caudovirales phages active against this important pathogen [21,[25][26][27][28]. These two phages share morphological features, genomic structure and moderate sequence similarity with Rhodococcus phage Toil.…”

Section: Introductionmentioning

confidence: 99%

A Novel Genus of Actinobacterial Tectiviridae

Caruso

deCarvalho

Huynh

et al. 2019

Viruses

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Streptomyces phages WheeHeim and Forthebois are two novel members of the Tectiviridae family. These phages were isolated on cultures of the plant pathogen Streptomyces scabiei, known for its worldwide economic impact on potato crops. Transmission electron microscopy showed viral particles with double-layered icosahedral capsids, and frequent instances of protruding nanotubes harboring a collar-like structure. Mass-spectrometry confirmed the presence of lipids in the virion, and serial purification of colonies from turbid plaques and immunity testing revealed that both phages are temperate. Streptomyces phages WheeHeim and Forthebois have linear dsDNA chromosomes (18,266 bp and 18,251 bp long, respectively) with the characteristic two-segment architecture of the Tectiviridae. Both genomes encode homologs of the canonical tectiviral proteins (major capsid protein, packaging ATPase and DNA polymerase), as well as PRD1-type virion-associated transglycosylase and membrane DNA delivery proteins. Comparative genomics and phylogenetic analyses firmly establish that these two phages, together with Rhodococcus phage Toil, form a new genus within the Tectiviridae, which we have tentatively named Deltatectivirus. The identification of a cohesive clade of Actinobacteria-infecting tectiviruses with conserved genome structure but with scant sequence similarity to members of other tectiviral genera confirms that the Tectiviridae are an ancient lineage infecting a broad range of bacterial hosts.

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Assessment of the Detrimental Impact of Polyvalent Streptophages Intended to be Used as Biological Control Agents on Beneficial Soil Streptoflora

Cited by 10 publications

References 71 publications

Volume 43 Number 1

Volume 43 Number 1

Detection and control of off-flavour compound-producing streptomycetes on locally produced nuts using streptophages

A Novel Genus of Actinobacterial Tectiviridae

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