Assessment of the cytotoxic potential of an aqueous-ethanolic extract fromThalassia testudinumangiosperm marine grown in the Caribbean Sea

Abstract

Thalassia testudinum extract is more cytotoxic and produced more DNA damage on human hepatoma cells than to other non-tumour cells. A possible mechanism is suggested for extract-induced cytotoxicity based on oxidative stress, nuclear damage and hypercalcaemia in HepG2 cells. T. testudinum may be a source for antitumour agents.

Polyphenolic Fraction Obtained From Thalassia testudinum Marine Plant and Thalassiolin B Exert Cytotoxic Effects in Colorectal Cancer Cells and Arrest Tumor Progression in a Xenograft Mouse Model

“…Recent work of our group provided evidence of the cytotoxic and DNA damage of the crude extract of T. testudinum on tumor cell lines. Additionally, this work suggested that the cytotoxic effect of the extract is associated with OS, nuclear damage, and sustained hypercalcaemia in HepG2 cells (Rodeiro et al, 2018). Along the same line, a chloroform fraction from T. testudinum was found to elicit cytotoxicity and antiproliferative effects on A549 and EA.hy926 immortalized cells as well (Riera et al, 2018).…”

“…IC 50 (concentration of the compounds that is required for 50% inhibition cell viability) values were determined from the best-fit of the Hill slope curve to experimental data using nonlinear regression analysis by using five different sample concentrations. To determine the cytotoxic selectivity index of products, the selectivity index (SI) was (Regalado et al, 2012;Rodeiro et al, 2018). determined using the formula: SI IC 50 of non cancer cell/IC 50 of cancer cell, where a SI ≥ 10 was considered a selective compound (Peña-Morán et al, 2016).…”

“…Thalassia testudinum seagrass (T. testudinum) has potential therapeutic use (Garateix et al, 2011;Regalado et al, 2012;García et al, 2017;Riera et al, 2018;Rodeiro et al, 2018), which in a near future can be approached as a nutraceutical product. The phytochemical characterization of an aqueous-ethanolic extract as well as the polyphenolic fraction from T. testudinum grown in the Cuban coasts showed a complex mixture containing phenols, flavonoids, proanthocyanidins, among others structures with cytotoxic effects (Table 1) (Regalado et al, 2012;Rodeiro et al, 2018;Riera et al, 2018). The glycosylated sulphated flavone thalassiolin B (chrysoeriol 7-β-D-glucopyranosyl-2"sulphate) (TB) has been identified as the main component found in T. testudinum hydroethanolic extract (5.8% w/w) ( Figure 1) (Regalado et al, 2009;Garateix et al, 2011;Regalado et al, 2012).…”

Interaction of Thalassia testudinum Metabolites with Cytochrome P450 Enzymes and Its Effects on Benzo(a)pyrene-Induced Mutagenicity

“…Th shows in vitro scavenger activity for • OH, RO 2 • , O 2 •− and DPPH • free radicals and in vivo antioxidant effects against brain and liver induced-lipid peroxidation in mice [ 34 , 35 ]. In addition, Th shows acute anti-inflammatory effects in mice [ 36 ] and it displays selective anti-proliferative activity against cancer cells compared to normal cells [ 37 ]. Besides, the extract also inhibits drug efflux by ABCG2/breast cancer resistance protein (BCRP) and ABCB1/P-glycoprotein (MDR1 gene), increasing intracellular accumulation of anticancer agents [ 38 , 39 ].…”

Interaction of Thalassia testudinum Metabolites with Cytochrome P450 Enzymes and Its Effects on Benzo(a)pyrene-Induced Mutagenicity

“…• , O2 •− and DPPH • free radicals and in vivo antioxidant effects against brain and liver induced-lipid peroxidation in mice [34,35]. In addition, Th shows acute antiinflammatory effects in mice [36] and it displays selective anti-proliferative activity against cancer cells compared to normal cells [37]. Besides, the extract also inhibits drug efflux by ABCG2/breast cancer resistance protein (BCRP) and ABCB1/P-glycoprotein (MDR1 gene), increasing intracellular accumulation of anticancer agents [38,39].…”