Assessment Of The Conformity Of The Methods For Aflatoxin B1 And Deoxynivalenol Determination In Grain And Feeds By Method Of High-Performance Liquid Chromatography

Yakubchak, O. M.; Laposha, O.A.; Мидык, С. В.; Taran, T. V.; Zabarna, I. V.

doi:10.17721/moca.2018.121-130

Cited by 5 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Такий підхід пов'язаний із основним недоліком цього методу, а саме, з можливістю перехресної реактивності (крос-реактивності) з подібними сполуками [16]. Тому з метою уникнення ризику отримання хибнопозитивних результатів, застосовують арбітражні хроматографічні та мас-спектрометричні методи [17].…”

Section: національний університет біоресурсів і природокористування уunclassified

“…Одним із сучасних методів, який дозволяє здійснювати багатокомпонентний аналіз в складних матрицях -є метод високоефективної рідинної хроматографії з мас-спектрометричним детектуванням (LC-MS/MS). Як відомо, багатокомпонентний аналіз є можливим завдяки тому, що розділення речовин відбувається у декількох площинах: з одного боку -розділення речовин на хроматографічній колонці, а з іншого -розділення речовин за їх співвідношенням маси до заряду (m/z) [16,17].…”

Section: національний університет біоресурсів і природокористування уunclassified

“…З метою усунення матричних ефектів використовують для побудови градуювальної кривої не чистий розчинник, а екстракт з матриці (наприклад, молока). Таким чином, використання матричних градуювальних кривих для кількісної оцінки мікотоксинів дасть більш точніші результати аналізу, оскільки враховується матричний ефект [16,17,20].…”

Section: національний університет біоресурсів і природокористування уunclassified

See 2 more Smart Citations

Comparative analysis of the effectiveness of analytical methods for the determination of aflatoxins in milk and dairy products (review information)

Senin

Данчук

Мидык

et al. 2020

Nauk. vìsn. vet. med.

View full text Add to dashboard Cite

The dairy industry of Ukraine is developing dynamically, its needs for the quality of raw materials are growing significantly. Detection of mycotoxins in raw milk is one of the main indicators of its safety. The high degree of toxicity of mycotoxins is a threat to the health of the lactating animal, so a large number of them are excreted in milk. If we talk about ruminants, the vast majority of mycotoxins are utilized by microorganisms of the pancreas, which does not occur in monogastric animals, so the list of mycotoxins in their milk can be much wider than the secretion of mammalian mammals. To date, the maximum permissible levels (MRLs) of mycotoxins in raw milk and dairy products have been established. Thus, a comprehensive determination of the content of mycotoxins in the secretion of the breast has not only technological but also important diagnostic value. Milk sample preparation is the most important step in the determination of mycotoxins and consists of sampling, extraction and purification from impurities. For the extraction of aflatoxins, the method of liquid extraction with acetonitrile or chloroform is used. Purification of extracts is carried out on immunoaffinity columns, cartridges with special sorbents or using certain manufacturers (MycoSep®).Enzyme-linked immunosorbent assay and high-performance liquid chromatography with fluorescence detection are used to determine aflatoxin B1 and M1 in raw milk of cows. However, all these methods have a number of disadvantages, namely: long and expensive sample preparation and insufficiently high selectivity. Currently, the complex determination of mycotoxins in various matrices by high-performance liquid chromatography with mass spectrometric detection (LC-MS/MS) and the use of modified QuEChERS sample preparation is gaining popularity. The advantage of this technique is the combination of faster and cheaper sample preparation of QuEChERS samples with highly selective LC-MS/MS chromatography. Key words: mycotoxins, raw milk, analytical methods, QuEChERS.

show abstract

Section: національний університет біоресурсів і природокористування уunclassified

See 1 more Smart Citation

Comparative analysis of the effectiveness of analytical methods for the determination of aflatoxins in milk and dairy products (review information)

Senin

Данчук

Мидык

et al. 2020

Nauk. vìsn. vet. med.

View full text Add to dashboard Cite

show abstract

“…Evaluation of food products' safety lies in determination of toxic substances (Yakubchak et al, 2018) and residues of veterinary drugs used in the process of productive animal breeding. Avermectins (AVM) are the veterinary drugs widely used in animal and cattle breeding for prevention and treatment of diseases caused by endo-and ectoparasites.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Ultra-High-Performance Liquid Chromatography (HPLC) Tandem Mass Spectrometry for Determination of Avermectin Residues in Milk

Bayer¹,

Kaminska²,

Bondarets³

et al. 2019

Ukr J Ecol

View full text Add to dashboard Cite

The study was conducted to evaluate the applicability of ultra high-performance liquid chromatography - tandem mass spectrometry method (UHPLC-MS/MS), establish the MS/MS detection parameters and determine the validation characteristics for the analysis of residual content of avermectins in milk. The UHPLC-MS/MS method has proved to be accurate, practical and universal. This was confirmed by Decision limit (CCα) data: abamectin - 12.56 μg/kg, doramectin - 17.74 μg/kg, eprinomectin - 24.02 μg/kg, ivermectin - 12.53 μg/kg, moxidectin - 44.69 μg/kg, recovery is 88.7-110%. The data obtained for assessing the suitability, accuracy and reproducibility of the results meet the requirements of the European Directive (2002/657/EC). The efficient ultra high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method that was developed and adopted for routine use by the laboratories of veterinary medicine, allows to detect residual quantities of 5 avermectins used in animal breeding for the prevention of helminthiases in food products including milk.

show abstract

Determination Of T-2 And HT-2 Toxin In Wheat Grain By HPLC With Fluorescence Detection

Laposha

Senin

Мидык

et al. 2020

MOCA

View full text Add to dashboard Cite

The procedure of the T-2 and HT-2 toxins determination in wheat grain was developed by high performance liquid chromatography according to the following criteria: specificity, linearity, limits of detection (LOD), limits of quantification (LOQ), trueness (recovery), precision, stability. It was found that the correlation coefficient (R2) for T-2 and HT-2 toxin was 0.9999. Mean recoveries from (R, %) for T-2 and HT-2 toxin at the level of 50–150 μg·Kg-1 from wheat (sample blank) were 91 and 87 %, respectively. The relative standard deviation (RSD, %) of the measurement results under conditions of repeatability and intra-laboratory precision ranged from 0.23 to 3.93 %. The limits of quantification of the method for T-2 and HT-2 toxin was 2.2 and 1.2 μg·Kg-1, respectively. These data are within the range of acceptable minimum levels in accordance with Commission Regulation (EC) No 401/2006. It is confirmed that the standards of T-2, HT2 toxins in solutions can be stored up to 7 days in a freezing chamber, a refrigerator and at room temperature in a tightly closed container without actual loss of concentration. It was found that T-2 and HT-2 in a solution cannot be stored in a lit place, because according to the data obtained, sunlight leads to the destruction of these mycotoxins by 52 % and 59 %, respectively.

show abstract

Assessment Of The Conformity Of The Methods For Aflatoxin B1 And Deoxynivalenol Determination In Grain And Feeds By Method Of High-Performance Liquid Chromatography

Cited by 5 publications

References 0 publications

Comparative analysis of the effectiveness of analytical methods for the determination of aflatoxins in milk and dairy products (review information)

Comparative analysis of the effectiveness of analytical methods for the determination of aflatoxins in milk and dairy products (review information)

Evaluation of Ultra-High-Performance Liquid Chromatography (HPLC) Tandem Mass Spectrometry for Determination of Avermectin Residues in Milk

Determination Of T-2 And HT-2 Toxin In Wheat Grain By HPLC With Fluorescence Detection

Contact Info

Product

Resources

About