Assessment of the clinical significance of serum matrix metalloproteinases MMP-2 and MMP-9 in patients with various chronic liver diseases and hepatocellular carcinoma

Abstract

SummaryMatrix metalloproteinases (MMPs) have the ability to degrade basement membranes and may thus play an important role in extracellular matrix turnover in liver fibrosis and carcinogene-sis. Serum levels of MMPs have been suggested as diagnostic markers in these processes.We measured serum MMP-2 and MMP-9 by ELISA in 91 patients with chronic liver disease, including 25 patients with hepatocellular carcinoma (HCC), and in 60 controls.MMP-2 was significantly higher in patients with chronic liver disease compared to controls, and increased with Child-Pugh class. There was a significant correlation between MMP-2 and liver function (bilirubin, albumin, and prothrombin time), and a strong opposite correlation between MMP-9 and these parameters.MMP-2 levels in patients with HCC were significantly higher than in controls, but comparable to patients with chronic liver disease without this malignancy. MMP-9 yielded no significant differences between patients with or without HCC and controls.Serum MMP-2 and to a lesser extent MMP-9 correlate with the severity of liver disease and may reflect changes in extracellular matrix remodeling. Due to a considerable overlap in patients with chronic liver disease with or without HCC, MMP-2 and MMP-9 can not be used as a diagnostic marker for HCC.Theme paper: Part of this paper was originally presented at the joint meetings of the 16th International Congress of the International Society of Fibrinolysis and Proteolysis (ISFP) and the 17th International Fibrinogen Workshop of the International Fibrinogen Research Society (IFRS) held in Munich, Germany, September, 2002.

Hepatitis C Virus as risk factor for development of hepatocellular carcinoma in Egypt: II-Enhancement role of matrix metalloproteinases-2 in dissemination of HCC

“…These data are going in agreement with Kuyvenhoven et al, (2003) (22) who found a significant correlation between MMP-2 and liver function (bilirubin, albumin, SGPT and prothrombin time) concluding that serum MMP-2 correlates with the severity of liver disease and may reflect changes in extracellular matrix remodeling.…”

MMP-2 and MMP-9 Serum Levels Change but their Gene Promoter Polymorphisms are not Associated with Late Phase I/R Injury or Rejection after Orthotopic Liver Transplantation

“…Obviously, due to the donor-recipient differences, the frequency of a mismatch in MMP-2 was significantly higher than in MMP-9 (57.8% versus 33.9 %, Table 2). Serum levels of MMP-2 are increased in patients with chronic liver disease and therefore a drop of MMP-2 levels was expected after OLT [11]. This decrease indeed was seen, as shown in Fig.…”

“…Increased mRNA expression of MMP-2 was reported in liver biopsy samples of patients with cirrhosis [10]. In addition, serum levels of MMP-2 are found to be increased in patients with chronic liver disease and to correlate with the severity of the liver function impairment [11]. MMP-9 is released predominantly from neutrophils and macrophages, but the principal source in the liver is thought to be the Kupffer cell, the resident macrophage of the liver [12].…”

Infliximab treatment influences the serological expression of matrix metalloproteinase (MMP)-2 and -9 in Crohnʼs disease

“…30,32,35 In brief, a polyclonal anti-MMP-2 antibody or monoclonal anti-MMP-9 antibody was used as catching antibody and appropriately diluted samples were incubated overnight at 4°C. Immunodetection of MMP-2 was performed using polyclonal anti-MMP-2 followed by biotin-labeled goat antirabbit-IgG and of MMP-9 with biotin-labeled polyclonal anti-MMP-9 antibody.…”