Assessment of the Biotechnological Potential of Cyanobacterial and Microalgal Strains from IPPAS Culture Collection

Optimization of CO2 Supply for the Intensive Cultivation of Chlorella sorokiniana IPPAS C-1 in the Laboratory and Pilot-Scale Flat-Panel Photobioreactors

“…On the third day of cultivation, when the samples were taken, the cultures had different biomass concentrations and were in different growth stages. The highest protein content is usually observed in exponentially growing cells [ 20 ], and exponential growth is possible when the cells are not limited in their light and nutrients [ 34 ]. Considering that all cultures were grown in the same medium and under the same average irradiance, the cultures with a lower biomass concentration were less limited with light and nutrients and, thus, were enriched in proteins at the expense of starch.…”

“…The samples for dry weight estimation were incubated overnight at 80 °C in pre-weighted plastic microtubes and then weighed. The detailed procedures of protein and starch extraction and determination of their concentration are described in [ 20 ]. Briefly, the total protein concentration was estimated with BCA assay, and the starch concentration was measured using the phenol-sulphuric method.…”

“…Intensive cultivation of microalgae is a promising method to capture and utilize the excess carbon dioxide [ 1 , 2 ]. As a result, the task of carbon footprint reduction with the simultaneous production of high-value products, theoretically, has been solved [ 3 , 4 , 5 ] and reflected in a solid number of publications [ 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 ]. It was demonstrated that, depending on a variety of parameters and cultivation conditions, a high carbon fixation rate (CFR) can be achieved [ 6 , 7 ].…”

“…Our previous studies on the cultivation of various strains of microalgae and cyanobacteria in the laboratory system made it possible to determine their technological ranges, which are suitable for the intensive cultivation of a wide range of photosynthetic microorganisms [ 20 ]. Those results provided the initial experimental data for the design of scalable flat-panel vertical PBRs with a high biomass yield [ 24 ] and allowed for the refinement of the technologies for the cultivation of photosynthetic microorganisms.…”

Study of the Polysaccharide Production by the Microalga Vischeria punctata in Relation to Cultivation Conditions

“…Additionally, the majority of the fat components are polyunsaturated fatty acids, and some of these algae have been found to contain significant amounts of valuable eicosapentaenoic acid [ 10 ]. Under stress conditions, these microalgae are known to be a superproducer of eicosapentaenoic fatty acid [ 11 , 12 ]. Schnepf et al [ 13 ] notes that all Eustigmatophyceae are characterized by the presence of so-called refractive granules, which are a storage substance of a β-1-3-linked polysaccharide.…”