Assessment of the anxiolytic activity of phospholipids derived from eggs enriched in n-3 polyunsaturated fatty acids (PUFA) in rat behavioral studies

Rutkowská, M; Słupski, Wojciech; Trocha, Małgorzata; Szandruk, Marta; Rymaszewska, Joanna

doi:10.1016/s1734-1140(13)71380-x

Cited by 1 publication

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“…Notably, PUFAs are abundant in EyPC. 16 EyPC has been widely used in the study of lipid peroxidation using model membranes. 17−21 The focus of this study was to investigate the effects of LA, a drug that increases the fluidity of lipid membranes, on the inhibition of lipid peroxidation occurring in the EyPC bilayer using the 2-thiobarbituric acid reactive species (TBARS) method.…”

Section: T H Imentioning

confidence: 99%

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Effects of Local Anesthetics on Liposomal Membranes Determined by Their Inhibitory Activity of Lipid Peroxidation

et al. 2023

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In this study, we investigated the effects of drugs on membrane function in which lipid peroxidation was inhibited by the antioxidant Trolox (TRO) in liposomes containing egg yolk lecithin. Local anesthetics (LAs), such as lidocaine (LID) and dibucaine (DIB), were used as model drugs. The effect of LAs on the inhibitory activity of TRO was evaluated by calculating the pI 50 from the inhibition constant K calculated by curve fitting. pI 50 TRO indicates the strength of TRO membrane protective function. pI 50 LA indicates the strength of LA activity. LAs inhibited lipid peroxidation in a dose-dependent manner and decreased pI 50 TRO. The effect of DIB on pI 50 TRO was 1.9 times more than that of LID. This result indicated that LA may improve the fluidity of the membrane, which may facilitate the migration of TRO from the membrane to the liquid phase. As a result, TRO is less likely to suppress lipid peroxidation within the lipid membrane, possibly resulting in a decrease in pI 50 TRO. The effect of TRO on pI 50 LA was found to be similar in both, indicating that it did not depend on the type of the model drug. These results suggest that our developed procedure successfully quantified the effects of LAs on lipid membrane functions. We were able to obtain the characteristics of model drugs independent of TRO by simultaneously measuring and analyzing the lipid peroxidation inhibitory activities of TRO and model drugs in liposomes.

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Section: T H Imentioning

confidence: 99%

“…In this study, egg yolk lecithin (EyPC) liposomes were used to mimic the plasma cell membrane. Notably, PUFAs are abundant in EyPC . EyPC has been widely used in the study of lipid peroxidation using model membranes. − …”

Section: Introductionmentioning

confidence: 99%