Assessment of the Antimutagenic Effects of Aqueous Extracts from Herbal Medicines against N-Alkyl-N-nitrosoureas-induced Mutagenicity Using the umu Test

Inami, Keiko; Takada, M; Itoh, Kei; Ishikawa, Satoko; Mochizuki, Masahito

doi:10.3123/jemsge.2014.003

Cited by 2 publications

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References 47 publications

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“…Pharmacologically, it exhibits anti-inflammatory [14, 15] and antitumour activities [16, 17]. Our previous study showed that an aqueous extract of S. suberectus Dunn showed the most potent antimutagenic effects against MNU by umu test screening [18].…”

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confidence: 99%

Antimutagenic components in Spatholobus suberectus Dunn against N-methyl-N-nitrosourea

et al. 2019

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BackgroundAn extract from Spatholobus suberectus (S. suberectus) Dunn has been reported to show potent antimutagenic effects against N-alkyl-N-nitrosoureas in umu screening. The aim of this study was to identify the antimutagenic components from extracts of S. suberectus against N-methyl-N-nitrosourea (MNU) in the Ames assay with Salmonella typhimurium strain TA1535 and to elucidate the antimutagenic mechanism of the flavonoids.ResultsFrom the ethyl acetate fraction obtained from fractionation of the methanol extract of S. suberectus Dunn, medicarpin, formononetin and isoliquiritigenin were successfully isolated through a combination of normal- and reversed-phase chromatography. Genistein and naringenin, which were already reported to be contained in S. suberectus Dunn, were also tested for their antimutagenicity towards MNU, along with formononetin, isoliquiritigenin and medicarpin. Our results demonstrated that genistein, isoliquiritigenin, medicarpin and naringenin were antimutagenic against MNU without showing cytotoxicity. MNU is reported to cause not only DNA alkylation but also induce reactive oxygen species. The hydroxyl radical scavenging capacity of the flavonoids was correlated with the antimutagenic capacity, indicating that the hydroxyl radical scavenging activity was involved in their antimutagenicity towards MNU.ConclusionsIt is important to prevent DNA damage by N-nitrosamines for cancer chemoprevention. Genistein, isoliquiritigenin, medicarpin and naringenin were demonstrated to possess an antigenotoxic effects against carcinogenic MNU due to their radical scavenging activity.

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confidence: 99%

Antimutagenic components in Spatholobus suberectus Dunn against N-methyl-N-nitrosourea

et al. 2019

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Antimutagenic components in Glycyrrhiza against N-methyl-N-nitrosourea in the Ames assay

Inami

Mine

Kojo

et al. 2016

Natural Product Research

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Antimutagenesis against N-nitroso compounds contribute to prevention of human cancer. We have found that Glycyrrhiza aspera ethanolic extract exhibits antimutagenic activity against N-methyl-N-nitrosourea (MNU) using the Ames assay with Salmonella typhimurium TA1535. In the present study, eight purified components from Glycyrrhiza, namely glabridin, glycyrrhetinic acid, glycyrrhizin, licochalcone A, licoricesaponin H2, licoricesaponin G2, liquiritigenin and liquiritin were evaluated for their antimutagenicity against MNU in the Ames assay with S. typhimurium TA1535. Glycyrrhetinic acid, glycyrrhizin, licoricesaponin G2, licoricesaponin H2 and liquiritin did not show the antimutagenicity against MNU in S. typhimurium TA1535. Glabridin, licochalcone A and liquiritigenin reduced revertant colonies derived from MNU in S. typhimurium TA1535 without showing cytotoxic effects, indicating that these compounds possess antimutagenic activity against MNU. The inhibitory activity of glabridin and licochalcone A was more effective than that of liquiritigenin. Thus, Glycyrrhiza contains antimutagenic components against DNA alkylating, direct-acting carcinogens.

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Assessment of the Antimutagenic Effects of Aqueous Extracts from Herbal Medicines against N-Alkyl-N-nitrosoureas-induced Mutagenicity Using the umu Test

Cited by 2 publications

References 47 publications

Antimutagenic components in Spatholobus suberectus Dunn against N-methyl-N-nitrosourea

Antimutagenic components in Spatholobus suberectus Dunn against N-methyl-N-nitrosourea

Antimutagenic components in Glycyrrhiza against N-methyl-N-nitrosourea in the Ames assay

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