Assessment of the antimicrobial activity of the lipoidal and pigment extracts of Punica granatum L. leaves

Elbatanony, Marwa M.; El-Feky, Amal M.; Hemdan, Bahaa A.; El‐Liethy, Mohamed Azab

doi:10.1016/j.chnaes.2018.05.003

Cited by 25 publications

(15 citation statements)

References 18 publications

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“…In our work we attempted to discover the correlation of the lipoidal and phenolic compounds and their impact on some human cell line carcinoma. An extensive number of hydrocarbons and triterpenoids with different groups are known to exhibit chemoprevention and cytotoxicity against many tumor cells as well as anticancer remedy both in vitro and in vivo [24][25][26] Quantitative structure-activity relationship studies on the cytotoxic effect of phenolic compounds have been examined in the recent period by many studies [27][28][29]. Hesperidin which is a major flavonoid in the ethyl acetate fractions of both plants under study, has demonstrated to protectively affect CCl 4 -induced oxidative stress and resultant dysfunction of rat liver which has been correlated to its antioxidant property [30].…”

Section: Discussionmentioning

confidence: 99%

Anti-cancer potential of the lipoidal and flavonoidal compounds from Pisum sativum and Vicia faba peels

El-Feky

Elbatanony

Mounier

2018

Egyptian Journal of Basic and Applied Sciences

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For a long time, plant secondary metabolites have been strongly examined for their antitumor and cytotoxic impacts. These days, there is another pattern of making utilization of the waste products of plants because of their extravagance of numerous phytochemical components and adequacy on human wellbeing. This research work is handling the effect of diversity of lipoidal and phenolic compounds found in the peels of two common edible plants in the Middle East; Pisum sativum and Vicia faba L. for their assesment as anticancer agents. The GC/MS of the n-hexane extract of both plant peels led to identification of twenty compounds (82.99%) and seventeen compounds (85.97%) of the total lipoidal contents from P. sativum and V. faba, respectively. While the HPLC analysis of the ethyl acetate fraction of the two plant peels resulted in recognition of 17 flavonoids and 18 phenolics from P. sativum and 16 flavonoids and 17 phenolics from V. faba. Moreover, four flavonoidal compounds were isolated to our knowledge for the first time from the peels and tested separately against different human cancer cell lines and the mode of action of the most potent compound has been determined. P. sativum ethyl acetate fraction possessed the highest scavenging activity (31.2%) as well as the most cytotoxic effect on breast carcinoma cell line. Apigenin proved to be the most potent tested compound on (MCF-7) and has no cytotoxic effect on normal human skin cell lines.

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Section: Discussionmentioning

confidence: 99%

Anti-cancer potential of the lipoidal and flavonoidal compounds from Pisum sativum and Vicia faba peels

El-Feky

Elbatanony

Mounier

2018

Egyptian Journal of Basic and Applied Sciences

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“…Dried PoP powder has been pretty good for treating many disorders, particularly headaches and stomach problems (Gullon et al ., ). PoP powder has been phenomenal in treating bleeding gums as well as plaques and also has promising AMA (Elbatanony et al ., ). PoP contains polyphenols that combat microbial pathogens and cause least or no toxicity to host organisms.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial potential of pomegranate peel: a review

Singh

Kaur

et al. 2018

Int J of Food Sci Tech

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Pomegranate peel (PoP) is a byproduct of the fruit juice processing industry, comprising nearly 30-40% of fruit portion. PoP is rich in polyphenols (phenolic acids, tannins and flavonoids particularly anthocyanins) which are known to have diverse biological functions including effectiveness against pathogenic microorganisms. PoP has shown a broad spectrum antimicrobial activity (AMA) of PoP against both Gram-positive and Gram-negative bacteria. It also presented promising AMA against antibiotic resistant microbial strains such as methicillin-resistant Staphylococcus aureus. The high levels of polyphenols, particularly punicalagin and ellagic acid, present in PoP have been responsible for its antifungal properties. PoP is effective against many fungi including both pathogenic (Aspergillus flavus) and opportunistic pathogens. These activities of PoP may be exploited as a phytomedicine for humans, in order to eliminate the use of antibiotics and reducing their cost. This review provides collective up-to-date information on the efficacy of plant-derived AMA of PoP.Pomegranate peel antimicrobial activity B. Singh et al. 960

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“…Next, the samples were suspended in 1 mL of 96% ethanol, mixed using a Vibromix, and centrifuged for 10 min at 20˚C and 12000 × g. The obtained supernatants were separated from the remaining silica gel particles, transferred into clean Eppendorf tubes, and centrifuged again under the previously described conditions. All extraction steps were performed in the dark to avoid cis-trans photoisomerization and photodestruction because chlorophylls and carotenoids are light-and heat-sensitive [20,21].…”

Section: Thin-layer Chromatography (Tlc)mentioning

confidence: 99%

“…The application of TLC resulted in the separation of major and minor chlorophyll and carotenoid components, leading to a reduced overlap of absorption peaks in the blue-green region during the spectrophotometric assay [20,[37][38][39]. Consequently, minor pigment component PLOS ONE absorption peaks were detected using a UV-Vis spectrophotometer.…”

Section: Differences In Estimated Pigment Concentrations Before and After Tlcmentioning

confidence: 99%

Enhanced pigment content estimation using the Gauss-peak spectra method with thin-layer chromatography for a novel source of natural colorants

Twardowska

2021

PLoS ONE

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Alternative pigment sources that are harmless to human health and can be produced in an eco-responsible way are of great research interest. The experiments undertaken in this study were conducted using autumn leaves of Aesculus hippocastanum as potential novel colorant sources. This study focused on improving the Gauss-peak spectra method (a less expensive alternative to high-pressure liquid chromatography) in combination with thin-layer chromatography, leading to the development of a new methodology. The collected leaves were stored at two different temperatures: 20°C and −20°C. The data obtained by spectrophotometric scanning of the samples were analyzed using the Gauss-peak spectra method in the R program with three wavelength ranges: 350–750 nm, 390–710 nm, and 400–700 nm. The results were then assessed for statistically significant differences in the estimated concentrations for the different wavelength ranges regarding (1) total pigment, carotenoid, and chlorophyll concentration (two-sample t-test) and (2) concentration of each indicated pigment (two-way analysis of variance). The results were also tested for differences between the estimated concentrations of samples stored under the different conditions. The Gauss-peak spectra results with and without thin-layer chromatography were statistically compared using a paired t-test. The results showed that thin-layer chromatography greatly enhanced the efficiency of the Gauss-peak spectra method for estimating the major and minor pigment composition without generating high additional costs. A wavelength range of 400–700 nm was optimal for all Gauss-peak spectra methods. In conclusion, the proposed method is a more successful, inexpensive alternative to high-pressure liquid chromatography.

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Assessment of the antimicrobial activity of the lipoidal and pigment extracts of Punica granatum L. leaves

Cited by 25 publications

References 18 publications

Anti-cancer potential of the lipoidal and flavonoidal compounds from Pisum sativum and Vicia faba peels

Anti-cancer potential of the lipoidal and flavonoidal compounds from Pisum sativum and Vicia faba peels

Antimicrobial potential of pomegranate peel: a review

Enhanced pigment content estimation using the Gauss-peak spectra method with thin-layer chromatography for a novel source of natural colorants

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