Assessment of the acute and sub-acute toxicity of the ethanolic extract of the aerial parts of Crassocephalum rabens (Asteraceae) in rats

Hsu, Pang-Kuei; Tsai, Yi Yu; Lin, Yu-Cheng; Kuan, Chen-Meng

doi:10.1016/j.toxrep.2021.12.005

Cited by 7 publications

(5 citation statements)

References 20 publications

(24 reference statements)

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“…The safety of the C. rabens extract has been verified by acute and sub-acute toxicity studies in rats, and the no-observed-adverse-effect-level (NOAEL) was reported to be greater than 1666.7 mg/kg body weight in male and female rats [30]. Accordingly, the acceptable daily intake (ADI) of the extract for a 60 kg adult is approximately 1 g, based on a 100-fold safety factor [31].…”

Section: Discussionmentioning

confidence: 98%

Ameliorating Effect of Crassocephalum rabens (Asteraceae) Extract on Skin Aging: A Randomized, Parallel, Double-Blind, and Placebo-Controlled Study

Kuan¹,

Liang

Chuang³

et al. 2022

Nutrients

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Crassocephalum rabens (Asteraceae) is a common herb used in Taiwanese folk medicine to treat inflammation-related syndromes. Pharmacological studies have revealed that galactolipids exhibit anti-oxidative, anti-inflammatory, and anti-hyaluronidase activities and improve skin wrinkles, moisture, and elasticity in healthy subjects. However, the anti-aging effects of C. rabens and its primary active compound, 1,2-di-O-linolenoyl-3-O-β-galactopyranosyl-sn-glycerol (dLGG), remain elusive. Here, we investigated whether C. rabens can improve skin conditions in healthy individuals using a double-blind approach. Forty enrolled volunteers were randomly and equally assigned to the control or treatment group and were required to take either a placebo or a C. rabens extract capsule daily for one month. Skin parameters were measured before and after the study. The results showed significant differences in skin elasticity, wrinkles, collagen content, brightness, and hydration between the baseline and week 4 in the treatment group. Particularly, compared with those in the placebo group, skin wrinkles (p < 0.05), brightness (p < 0.001), collagen content (p < 0.01), and UV spots (p < 0.05) were notably improved after treatment with the C. rabens extract. Our study successfully demonstrated the application of C. rabens in preventing skin aging. Further investigations will be conducted to study the underlying anti-aging mechanism of dLGG.

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Section: Discussionmentioning

confidence: 98%

Ameliorating Effect of Crassocephalum rabens (Asteraceae) Extract on Skin Aging: A Randomized, Parallel, Double-Blind, and Placebo-Controlled Study

Kuan¹,

Liang

Chuang³

et al. 2022

Nutrients

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“…are represented as mean±SEM (n=5 rats/group) Acute toxicity data show limited clinical application since cumulative toxic effects do occur even at low doses. Hence, repeated dosing toxicological evolution that is sub-acute toxicity study is almost always invaluable for the safety profile of phytomedicines [15]. Daily administration may cause accumulation in the body with gradual effects on tissues and organs rodents.…”

Section: Fig 1: Representation Of Sub-acute Toxicity Effect Of Methan...mentioning

confidence: 99%

Evaluation of Acute and Sub-Acute Toxicity of Methanolic Leaf Extract of Bambusa Vulgaris on Male Wistar Rats

SARKAR,

ROY CHOWDHURY,

CHOUDHURI

2024

Asian J Pharm Clin Res

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Objectives: The study aims to evaluate the potential toxicity of methanolic extract of leaves of Bambusa vulgaris in male Wistar rats. Methods: The methanolic extract was prepared for acute and sub-acute toxicity to determine the lethal dose. During the acute toxicity study, male rats were orally administered with leaf extracts at single doses of 100, 500,800, and 2000 mg/kg according to OECD Guidelines 425 for 14 days. Sub-acute toxicity dose at 200, 400, and 800 mg/kg was studied by daily dosing on male rats for 30 days. Results: The acute toxicity study revealed no lethal effects and behavioral signs of toxicity at the tested doses indicating that LD50 is <2000 mg/kg. In a sub-acute study, non-significant changes in the body weight (p<0.05), body weight gain percentage (p<0.05) food intake (p<0.05), and relative organ weight percentage (p<0.05), and hematological parameters (p<0.05) of the rats were observed. A significant (p<0.05) increase in ALT level was observed dose at 800 mg/kg. Histopathological analysis showed mild cell distortion in the liver and kidney in male rats treated at 800 mg/kg of extract. Conclusion: Our study suggests that oral administration of methanolic leaf extract Bambusa vulgais is safe. However, a dose of 800 mg/kg may be toxic for the liver and kidney.

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“…For the preclinical safety assessment of the probiotic yogurt, two in vivo toxicological tests were considered with albino rats: acute oral toxicity assessment (OECD, 2001;Hsu et al, 2021) and 28-day repeated dose toxicity assessment (OECD, 2008;Hsu et al, 2021).…”

Section: Conditioning Of Experimental Animalsmentioning

confidence: 99%

“…The study was conducted based on the OECD 423: 2001 Standard on Acute Oral Toxicity (Organisation for Economic Cooperation and Development, 2001;Hsu et al, 2021). Twelve male rats were randomized into two groups uniformly.…”

Section: Acute Oral Toxicity Assessmentmentioning

confidence: 99%

“…The study was conducted based on the OECD 407: 2008 Standard on Repeated Dose Oral Toxicity for 28 days (Organisation for Economic Cooperation and Development, 2008;Hsu et al, 2021) and previous results obtained from acute oral toxicity assessment. Twelve rats were randomized into two groups uniformly.…”

Section: Evaluation Of Oral Toxicity By Repeated Dose For 28 Daysmentioning

confidence: 99%

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Preclinical safety evaluation of a probiotic yogurt made with tumbo pulp (Passiflora tripartita Kunth)

Inocente Camones,

Arias Arroyo,

Flores López

et al. 2024

Braz. J. Food Technol.

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A probiotic yogurt made from the pulp of the tumbo fruit (Passiflora tripartita Kunth) possesses antioxidant capacity, physicochemical and microbiological quality and stability; however, it needs to be safe for later studies of clinical functionality. The present study would be considered as a precursor to the preclinical safety evaluation of a functional food by in vivo toxicological testing. The study aimed to evaluate the preclinical safety of yogurt made from the pulp of the tumbo fruit (P. tripartita). The toxicological studies proposed were based on the OECD 423:2001 standard on Acute Oral Toxicity, performing the peroral administration of the probiotic yogurt at a dose of 2000 mg/kg one-time in a group of randomized male rats, for its evaluation during 14 days compared to a control group; and on the OECD 407: 2008 on Repeated Dose Oral Toxicity, a daily dose of 2000 mg/kg of the yogurt was administered for 28 days in male rats, considering the evaluation of their clinical, hematological and biochemical parameters, to determine any possible toxic effect produced by the probiotic formulation. The results showed that in both toxicological studies, no adverse alterations were detected in their clinical signs, relative organ weights and hematological and biochemical profiles. In addition, a possible improvement in the immunological status, liver function and hematological profile was evidenced as a finding. In conclusion, it was possible to establish knowledge on the preclinical safety of the formulation of a probiotic yogurt made with tumbo fruit pulp, obtaining a NOAEL (No Observed Adverse Effect Level) value higher than 2000 mg/kg body weight in male rats.

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Assessment of the acute and sub-acute toxicity of the ethanolic extract of the aerial parts of Crassocephalum rabens (Asteraceae) in rats

Cited by 7 publications

References 20 publications

Ameliorating Effect of Crassocephalum rabens (Asteraceae) Extract on Skin Aging: A Randomized, Parallel, Double-Blind, and Placebo-Controlled Study

Ameliorating Effect of Crassocephalum rabens (Asteraceae) Extract on Skin Aging: A Randomized, Parallel, Double-Blind, and Placebo-Controlled Study

Evaluation of Acute and Sub-Acute Toxicity of Methanolic Leaf Extract of Bambusa Vulgaris on Male Wistar Rats

Preclinical safety evaluation of a probiotic yogurt made with tumbo pulp (Passiflora tripartita Kunth)

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