Assessment of synthetic chimeric multiple antigenic peptides for diagnosis of GB virus C infection

Gómara, María J.; Fernández, Leticia; Pérez, Teresa; Ercilla, Guadalupe; Haro, Isabel

doi:10.1016/j.ab.2009.09.011

Cited by 31 publications

(20 citation statements)

References 42 publications

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“…Unlike HCV, which elicits antibodies to several viral proteins during viraemia that usually persist throughout infection (Baumert et al , 2000), GBV-C/HGV antibodies are not generally detected during viraemia, although some studies have reported the detection of anti-GBV-C/HGV peptide reactivity (Fernandez-Vidal et al , 2007; Gomara et al , 2010; Pilot-Matias et al , 1996b; Schwarze-Zander et al , 2006; Tan et al , 1999; Van der Bij et al , 2005; Xiang et al , 1998). Following clearance of GBV-C/HGV viraemia, most individuals develop conformation-dependent antibodies to the envelope glycoprotein E2, and thus E2 antibody serves as a marker of prior infection (Barnes et al , 2007; Gutierrez et al , 1997; McLinden et al , 2006; Nakatsuji et al , 1992; Pilot-Matias et al , 1996a; Tacke et al , 1997; Tanaka et al , 1998).…”

Section: Virologymentioning

confidence: 99%

The GB viruses: a review and proposed classification of GBV-A, GBV-C (HGV), and GBV-D in genus Pegivirus within the family Flaviviridae

Stapleton

Foung

Muerhoff

et al. 2010

Journal of General Virology

253

327

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In 1967, it was reported that experimental inoculation of serum from a surgeon (G.B.) with acute hepatitis into tamarins resulted in hepatitis. In 1995, two new members of the family Flaviviridae, named GBV-A and GBV-B, were identified in tamarins that developed hepatitis following inoculation with the 11th GB passage. Neither virus infects humans, and a number of GBV-A variants were identified in wild New World monkeys that were captured. Subsequently, a related human virus was identified [named GBV-C or hepatitis G virus (HGV)], and recently a more distantly related virus (named GBV-D) was discovered in bats. Only GBV-B, a second species within the genus Hepacivirus (type species hepatitis C virus), has been shown to cause hepatitis; it causes acute hepatitis in experimentally infected tamarins. The other GB viruses have however not been assigned to a genus within the family Flaviviridae. Based on phylogenetic relationships, genome organization and pathogenic features of the GB viruses, we propose to classify GBV-A-like viruses, GBV-C and GBV-D as members of a fourth genus in the family Flaviviridae, named Pegivirus (pe, persistent; g, GB or G). We also propose renaming ‘GB’ viruses within the tentative genus Pegivirus to reflect their host origin.

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Section: Virologymentioning

confidence: 99%

The GB viruses: a review and proposed classification of GBV-A, GBV-C (HGV), and GBV-D in genus Pegivirus within the family Flaviviridae

Stapleton

Foung

Muerhoff

et al. 2010

Journal of General Virology

253

327

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“…Unfortunately, no commercial assay for the detection of GBV-C E2 antibodies is currently available. Future development of a GBV-C E2 antibody assay is very much needed in order to provide better insight into GBV-C infection status and thereby enabling better analysis of the impact of GBV-C infection on other viral diseases [40,41]. …”

Section: Gbv-c: Transmission Epidemiology and Detectionmentioning

confidence: 99%

Role of GB virus C in modulating HIV disease

Schwarze‐Zander

Blackard

Rockstroh

2012

Expert Review of Anti-infective Therapy

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GB virus C (GBV-C) is a member of the Flaviviridae family and the most closely related human virus to HCV. However, GBV-C does not replicate in hepatocytes, but rather in lymphocytes. GBV-C has a worldwide distribution and is transmitted sexually, parenterally and through mother-to-child transmission. Thus, co-infection with HCV and HIV is common. Until now, no human disease has been associated with GBV-C infection. However, there are several reports of a beneficial effect of GBV-C on HIV disease progression in vivo. Different mechanisms to explain these observations have been proposed, including modification of antiviral cytokine production, HIV co-receptor expression, direct inhibition of HIV-1 entry, T-cell activation and Fas-mediated apoptosis. Further understanding of these mechanisms may open new strategies for the treatment of HIV/AIDS.

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“…ELISA was performed as previously described . Using the LP1 linear peptide, we initially assessed the serologic reactivity of the 95 sera panel from HIV‐infected patients that previously tested positive for GBV‐C anti‐E2 protein antibodies by the Abbott test.…”

Section: Resultsmentioning

confidence: 99%

Synthetic peptides derived from an N‐terminal domain of the E2 protein of GB virus C in the study of GBV‐C/HIV‐1 co‐infection

Fernández

Chan

Egido

et al. 2012

Journal of Peptide Science

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Synthetic peptides derived from GB virus C (GBV-C) have previously been studied in our group for the development of new systems capable of diagnosing diseases caused by this humanotropic virus. We also recently described specific peptide domains of the E2 envelop protein of GBV-C that have the capacity to interfere with the HIV-1 fusion peptide, produce a notable decrease in cellular membrane fusion, and perturb HIV-1 infectivity in a dose-dependent manner. The present work discloses the design and synthesis of both linear and cyclic branched peptides based on a previously reported N-terminal sequence of the GBV-C E2 protein. Immunoassays and cell-cell fusion assays were performed to evaluate their diagnostic value to detect anti-GBV-C antibodies in HIV-1 patients, as well as their putative anti-HIV-1 activity as entry inhibitors. Our results showed that chemical modifications of the selected E2(7-26) linear peptide to afford cyclic architecture do not result in an enhanced inhibition of gp41 HIV-1-mediated cell-cell fusion nor improved sensitivity in the detection of GBV-C antibodies in HIV-1 co-infected patients. Thus, the ELISA data reinforce the potential utility of linear versions of the E2(7-26) region for the development of new peptide-based immunosensor devices for the detection of anti-GBV-C antibodies in HIV-1 co-infected patients.

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Assessment of synthetic chimeric multiple antigenic peptides for diagnosis of GB virus C infection

Cited by 31 publications

References 42 publications

The GB viruses: a review and proposed classification of GBV-A, GBV-C (HGV), and GBV-D in genus Pegivirus within the family Flaviviridae

The GB viruses: a review and proposed classification of GBV-A, GBV-C (HGV), and GBV-D in genus Pegivirus within the family Flaviviridae

Role of GB virus C in modulating HIV disease

Synthetic peptides derived from an N‐terminal domain of the E2 protein of GB virus C in the study of GBV‐C/HIV‐1 co‐infection

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