Assessment of synovial fluid biomarkers in healthy foals and in foals with tarsocrural osteochondrosis

Grauw, Janny C. de; Donabédian, Michaël; Lest, Chris H.A. van de; Perona, Giovanni Emilio; Robert, Céline; Lepage, Olivier; Martin‐Rosset, W.; Weeren, P. R. van

doi:10.1016/j.tvjl.2010.12.001

Cited by 18 publications

(15 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This recovery rate is consistent with prior reports of the use of the IBEX ELISA kit for analysis of synovial fluid (24-26). Dilution studies were not performed due to the relatively small volumes of synovial fluid obtained from the knees; however, these assays have been previously used in other reports of analysis of non-human synovial fluid (27-29). …”

Section: Resultsmentioning

confidence: 99%

Loss of extracellular matrix from articular cartilage is mediated by the synovium and ligament after anterior cruciate ligament injury

Haslauer

Elsaid

Fleming

et al. 2013

Osteoarthritis and Cartilage

View full text Add to dashboard Cite

Objective Post-traumatic osteoarthritis (PTOA) occurs after anterior cruciate ligament (ACL) injury. PTOA may be initiated by early expression of proteolytic enzymes capable of causing degradation of the articular cartilage at time of injury. This study investigated the production of three of these key proteases in multiple joint tissues after ACL injury and subsequent markers of cartilage turnover. Design ACL transection was performed in adolescent minipigs. Collagenase (MMP-1 and MMP-13) and aggrecanase (ADAMTS-4) gene expression changes were quantified in the articular cartilage, synovium, injured ligament, and the provisional scaffold at days 1, 5, 9, and 14 post-injury. Markers of collagen degradation (C2C), synthesis (CPII) and aggrecan synthesis (CS846) were quantified in the serum and synovial fluid. Histologic assessment of the cartilage integrity (OARSI scoring) was also performed. Results MMP-1 gene expression was upregulated in the articular cartilage, synovium and ligament after ACL injury. MMP-13 expression was suppressed in the articular cartilage, but upregulated 100fold in the synovium and ligament. ADAMTS-4 was upregulated in the synovium and ligament but not in the articular cartilage. The concentration of collagen degradation fragments (C2C) in the synovial joint fluid nearly doubled in the first five days after injury. Conclusion We conclude that upregulation of genes coding for proteins capable of degrading cartilage ECM is seen within the first few days after ACL injury, and this response is seen not only in chondrocytes, but also in cells in the synovium, ligament and provisional scaffold.

show abstract

Section: Resultsmentioning

confidence: 99%

Loss of extracellular matrix from articular cartilage is mediated by the synovium and ligament after anterior cruciate ligament injury

Haslauer

Elsaid

Fleming

et al. 2013

Osteoarthritis and Cartilage

View full text Add to dashboard Cite

show abstract

“…These limitations should be overcome by improved knowledge on articular tissue metabolism and molecular factors regulating its normal homeostasis. Proposed diagnostic markers were suggestive of an inflammatory and cartilage turnover condition [14][15][16][17] and proved partially effective in early disease diagnosis. In OA, for example, deregulated levels of IL-1β, IL-6 and TNFα, have been reported, although the functional role of these pro-and antiinflammatory mediators has still to be completely defined [18].…”

Section: Introductionmentioning

confidence: 99%

Gambling on putative biomarkers of osteoarthritis and osteochondrosis by equine synovial fluid proteomics

Chiaradia

Pepe

Tartaglia

et al. 2012

Journal of Proteomics

View full text Add to dashboard Cite

“…Elevated levels of eicosanoids leukotriene B 4 (LTB 4 ) and prostaglandin E 2 (PGE 2 ) could be related to synovial effusion in osteochondritis dissecans (OCD), while substance P proved to differentiate between subjects that did or did not respond to intraarticular diagnostic anesthesia. Combined, these studies also modified my view of the assumed direct relation between SF PGE 2 and joint pain: mature OCD-affected equine subjects were not lame but did have high PGE 2 levels, while lower PGE 2 levels were seen in young foals with OC versus controls (de Grauw et al 2011), and again higher SF PGE 2 was found in lame horses compared to sound controls but this was not related to the response to diagnostic joint anesthesia as an indicator of joint pain. As these findings indicate, it would be an oversimplification to assume a simple linear relation between SF concentrations of individual pro-nociceptive mediators and measures of joint pain; given the complex neural and inflammatory pathways involved in joint nociception and pain (van Weeren and de Grauw 2010), it is not surprising such a linear relation has not been found.…”

Section: Sf Biomarker Studies: Progress and Perspectivesmentioning

confidence: 92%

Molecular monitoring of equine joint homeostasis

Grauw

2011

Veterinary Quarterly

View full text Add to dashboard Cite

Diseases affecting synovial joints are a major cause of chronic disability both in humans and in companion animal species, most notably dogs and horses. As progressive deterioration of the articular cartilage is the hallmark of degenerative joint disease or osteoarthritis, research efforts traditionally tended to focus primarily on cartilage pathology. However, in recent years it has become clear that synovial joints should be considered intricate organs in their own right, with each of the constituent tissues (cartilage, bone, and synovial membrane) interacting with each other both in health and disease. Moreover, with the advent of modern molecular biology techniques, the importance of synovial inflammation in disease development and progression has become increasingly recognized. These realizations have spurred the need for tools that allow a more comprehensive, integral study of synovial joint homeostasis. This review provides a brief overview of synovial joint biology and the concept of joint homeostasis, followed by a discussion of methods that may be used to study joint homeostasis (varying from in vitro tissue culture to in vivo imaging) including specific advantages and limitations of each approach. It then zooms in on one such approach, synovial fluid biomarker analysis, as a promising avenue in synovial joint research, highlighting some results from equine studies performed in the author's own laboratory that illustrate how such studies may help shed light on in vivo joint homeostasis and therapeutic modulation thereof. The review concludes with some future perspectives and promising developments in the field.

show abstract

Assessment of synovial fluid biomarkers in healthy foals and in foals with tarsocrural osteochondrosis

Cited by 18 publications

References 41 publications

Loss of extracellular matrix from articular cartilage is mediated by the synovium and ligament after anterior cruciate ligament injury

Loss of extracellular matrix from articular cartilage is mediated by the synovium and ligament after anterior cruciate ligament injury

Gambling on putative biomarkers of osteoarthritis and osteochondrosis by equine synovial fluid proteomics

Molecular monitoring of equine joint homeostasis

Contact Info

Product

Resources

About