Assessment of Subepithelial Angiogenesis in Acquired Cholesteatoma between Pediatric and Adult Patients

Abstract

Objective: The aim of this study was to compare subepithelial angiogenesis developing within the perimatrix of the cholesteatoma between pediatric and adult patients. Methods:Sixty-one patients who underwent mastoidectomy for the first intent because of chronic otitis media with cholesteatoma between 1993 and 2013 and from whom appropriate tissue specimens were taken were included in the study. The patients were classified in the pediatric patient group if they were under the age of 18 years and the adult patient group if they were 18 years and older. Immunohistochemical staining for CD-31 was performed on new sections taken during surgery and sections prepared from archived tissues in paraffin blocks. Results were compared between the groups.Results: A total of 61 patients, of whom 25 were pediatric and 36 were adult patients, were included in the study. The mean CD-31 immunopositive microvessel rates were 8.8 (3-15) and 6.61 (2-14) for the pediatric and adult patient groups, respectively. The difference between the groups was statistically significant (p=0.037). Correlation analysis showed a statistically significant negative correlation between the CD-31 immunopositive microvessel rates and age (p=0.036). Conclusion:Subepithelial angiogenesis developing within the perimatrix of the cholesteatoma of the pediatric patients was more expressed than that of the adult patients. Keywords

The correlations between dynamic contrast enhanced magnetic resonance imaging and immunohistochemical data in head and neck squamous cell carcinomas

“…Immunohistochemical staining of the tissue sections in paraffin-embedded blocks was conducted, as described previously, with ki-67 (Roche Diagnostics., prediluted), CD34 (Roche Diagnostics., prediluted), and CD105 (Cornomics, 1/100 dilution) primary antibodies [ 6 ]. Digital images of the sections were evaluated on a computer via a camera attached to a light microscope.…”

“…Angiogenesis is a significant factor in tumor growth, and it is vital to observe and measure growth quantitatively to determine the malignant behavior of the tumor [ 4 ]. The quantitative analysis of angiogenesis could be performed using immunohistochemical methods and antibodies specific to the antigens found in vascular endothelium, such as CD34 and/or CD105 [ 5 , 6 ]. Although there are certain limitations, positron emission tomography can provide practical information, while perfusion imaging can provide information on capillarity; however, both methods lack adequate histopathological details [ 1 , 7 ].…”