Assessment of subchondral bone marrow lipids in healthy controls and mild osteoarthritis patients at 3T

Wang, Ligong; Salibi, Nouha; Chang, Gregory; Vieira, Renata; Babb, James S.; Krasnokutsky, Svetlana; Abramson, Steven B.; Regatte, Ravinder R.

doi:10.1002/nbm.1770

Cited by 14 publications

(26 citation statements)

References 28 publications

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“…The phantom replacement technique and coil loading correction using the transmitter amplitude of a 90° pulse or the reference transmitter amplitude are techniques that have been validated by others in the field (16,17) and have been commonly used for metabolite quantification with in vivo MRS. The external reference signal was used for normalization of in vivo lipid peaks (15). …”

Section: Methodsmentioning

confidence: 99%

“…Observed resonances include the olefinic (–CH═CH–) peak at 5.31 ppm, the methylene peaks at 1.3 ppm (–(CH 2 ) n –) and 2.03 ppm (–CH 2 –CH═CH–CH 2 –), and the methyl (–CH 3 ) peak at 0.9 ppm, which were fitted with Lorentzian lines. Peak integrals of these resonances were corrected for coil loading with the transmitter reference amplitude from individual measurements, and then used to normalize the methyl, methylene, and olefinic lipid signals to the corrected peak integral value of the external reference water phantom (15,16). Lateral femur was normalized to the top right voxel of the phantom, lateral tibia to the bottom right, medial femur to the top left, and medial tibia to the bottom left voxel of the phantom, respectively, using the following equation different from that in Refs.…”

Section: Methodsmentioning

confidence: 99%

“…Lateral femur was normalized to the top right voxel of the phantom, lateral tibia to the bottom right, medial femur to the top left, and medial tibia to the bottom left voxel of the phantom, respectively, using the following equation different from that in Refs. (1,2) in response to the water phantom replacement method (15,16) applied in the present work:

\frac{I false(0.9 ppm false)}{I false(phantom false)}, \frac{I false(1.3 ppm false)}{I false(phantom false)}, \frac{I false(2.03 ppm false)}{I false(phantom false)}, \frac{I false(5.31 ppm false)}{I false(phantom false)} .

…”

Section: Methodsmentioning

confidence: 99%

“…The normalized peak integrals were also used in the calculation of the index of unsaturation (2,15):

U = \frac{I false(5.31 ppm false)}{I false(5.31 ppm false) + I false(1.3 ppm false) + I false(2.03 ppm false) + I false(0.9 ppm false)} .

…”

Section: Methodsmentioning

confidence: 99%

“…Although previous studies have used the magnetic resonance spectroscopy (MRS) imaging method to measure the major components of bone marrow signals including water and lipids (1,2,15), to the best of our knowledge, there have been no quantitative assessments of bone marrow lipids alone in different femoral–tibial bone compartments in acute ACL-injured patients. Therefore, we hypothesized in this study that the compartment-specific subchondral lipid variations may be possible precursor of articular degeneration in acute ACL-injured patients, and the MRS of bone marrow signals may be helpful to characterize subchondral bone marrowamong healthy controls, acute ACL-injured patients, and patients with OA.…”

mentioning

confidence: 99%

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Evaluation of Subchondral Bone Marrow Lipids of Acute Anterior Cruciate Ligament (ACL)-Injured Patients at 3 T

et al. 2014

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Rationale and Objectives The objectives of this study were to investigate the changes in compartment-specific subchondral bone marrow lipids of femoral–tibial bone in acute anterior cruciate ligament (ACL)-injured patients compared to that of healthy volunteers and patients with osteoarthritis (OA) (Kellgren–Lawrence [KL] grade 2–3). Materials and Methods A total of 55 subjects were recruited in the study and subdivided into three subgroups: 17 healthy controls (4 females, 13 males; mean age = 41 ± 16, age range 24–78 years), 17 patients with acute ACL injury (3 females, 14 males; mean age = 30 ± 11, age range 18–61 years), and 21 patients with KL2–3 OA (12 females, 9 males; mean age = 65 ± 12, age range 44–89 years). Routine clinical proton density–weighted fast spin echo images in sagittal (without fat saturation), axial, and coronal (fat saturation) planes were acquired on a 3 T clinical scanner for cartilage morphology using Whole-Organ Magnetic Resonance Imaging Score grading. A voxel of 10 × 10 × 10 mm3 was positioned in the medial and lateral compartments of the tibia and femur for proton magnetic resonance spectroscopy measurements using the single voxel stimulated echo acquisition mode pulse sequence. All proton magnetic resonance data were processed with Java-based magnetic resonance user interface. Wilcoxon rank sum test and mixed model two-way analysis of variance were performed to determine significant differences between different compartments and examine the effect of ACL injury, OA grade and compartment, and their interactions. Results The index of unsaturation in lateral tibial compartment in ACL-injured patients was significantly higher (P < .05) than all compartments except lateral femoral in patients with KL2–3 OA. Significantly lower values (P < .05) were also identified in saturated lipids at 2.03 ppm in all compartments in ACL-injured patients than those of all compartments in patients with KL2–3 OA. Conclusions The preliminary results suggest that the indices of unsaturation in the lateral tibial compartment and the peaks of saturated lipids at 1.3 and 2.03 ppm in medial tibial compartment may be clinically useful to characterize subchondral bone marrow among healthy controls, acute ACL-injured patients, and patients with OA.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

\frac{I false(0.9 ppm false)}{I false(phantom false)}, \frac{I false(1.3 ppm false)}{I false(phantom false)}, \frac{I false(2.03 ppm false)}{I false(phantom false)}, \frac{I false(5.31 ppm false)}{I false(phantom false)} .

…”

Section: Methodsmentioning

confidence: 99%

“…The normalized peak integrals were also used in the calculation of the index of unsaturation (2,15):

U = \frac{I false(5.31 ppm false)}{I false(5.31 ppm false) + I false(1.3 ppm false) + I false(2.03 ppm false) + I false(0.9 ppm false)} .

…”

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Evaluation of Subchondral Bone Marrow Lipids of Acute Anterior Cruciate Ligament (ACL)-Injured Patients at 3 T

et al. 2014

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Imaging‐based assessment of fatty acid composition in human bone marrow adipose tissue at 7 T: Method comparison and in vivo feasibility

Emin

Oei

Englund

et al. 2023

Magnetic Resonance in Med

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To demonstrate the feasibility and accuracy of chemical shift-encoded imaging of the fatty acid composition (FAC) of human bone marrow adipose tissue at 7 T, and to determine suitable image-acquisition parameters using simulations. Methods:The noise performance of FAC estimation was investigated using simulations with a range of inter-echo time, and accuracy was assessed using a phantom experiment. Furthermore, one knee of 8 knee-healthy subjects (ages 35-54 years) was imaged, and the fractions of saturated fatty acids (SFA) and polyunsaturated fatty acids (PUFA) were mapped. Values were compared between reconstruction methods, and between anatomical regions.Results: Based on simulations, ΔTE = 0.6 ms was chosen. The phantom experiment demonstrated high accuracy of especially SFA using a constrained reconstruction model (slope = 1.1, average bias = −0.2%). The lowest accuracy was seen for PUFA using a free model (slope = 2.0, average bias = 9.0%). For in vivo images, the constrained model resulted in lower intersubject variation compared with the free model (e.g., in the femoral shaft, the SFA percent-point range was within 1.0% [vs. 3.0%]). Furthermore, significant regional FAC differences were detected. For example, using the constrained approach, the femoral SFA in the medial condyle was lower compared with the shaft (median [range]: 27.9% [27.1%, 28.4%] vs. 32.5% [31.8%, 32.8%]). Conclusion: Bone marrow adipose tissue FAC quantification using chemical-shift encoding is feasible at 7 T. Both the noise performance and accuracy of the technique are superior using a constrained signal model.

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Resolving the omega‐3 methyl resonance with long echo time magnetic resonance spectroscopy in mouse adipose tissue at 9.4 T

et al. 2020

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Tissue omega‐3 (ω‐3) content is biologically important to disease; however, its quantification with magnetic resonance spectroscopy in vivo is challenging due to its low concentration. In addition, the ω‐3 methyl resonance (≈ 0.98 ppm) overlaps that of the non‐ω‐3 (≈ 0.90 ppm), even at 9.4 T. We demonstrate that a Point‐RESolved Spectroscopy (PRESS) sequence with an echo time (TE) of 109 ms resolves the ω‐3 and non‐ω‐3 methyl peaks at 9.4 T. Sequence efficacy was verified on five oils with differing ω‐3 fat content; the ω‐3 content obtained correlated with that measured using 16.5 T NMR (R2 = 0.97). The PRESS sequence was also applied to measure ω‐3 content in visceral adipose tissue of three different groups (all n = 3) of mice, each of which were fed a different 20% w/w fat diet. The fat portion of the diet consisted of low (1.4%), medium (9.0%) or high (16.4%) ω‐3 fat. The sequence was also applied to a control mouse fed a standard chow diet (5.6% w/w fat, which was 5.9% ω‐3). Gas chromatography (GC) analysis of excised tissue was performed for each mouse. The ω‐3 fat content obtained with the PRESS sequence correlated with the GC measures (R2 = 0.96). Apparent T2 times of methyl protons were assessed by obtaining spectra from the oils and another group of four mice (fed the high ω‐3 diet) with TE values of 109 and 399 ms. Peak areas were fit to a mono‐exponentially decaying function and the apparent T2 values of the ω‐3 and non‐ω‐3 methyl protons were 906 ± 148 and 398 ± 78 ms, respectively, in the oils. In mice, the values were 410 ± 68 and 283 ± 57 ms for ω‐3 and non‐ω‐3 fats, respectively.

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Assessment of subchondral bone marrow lipids in healthy controls and mild osteoarthritis patients at 3T

Cited by 14 publications

References 28 publications

Evaluation of Subchondral Bone Marrow Lipids of Acute Anterior Cruciate Ligament (ACL)-Injured Patients at 3 T

Evaluation of Subchondral Bone Marrow Lipids of Acute Anterior Cruciate Ligament (ACL)-Injured Patients at 3 T

Imaging‐based assessment of fatty acid composition in human bone marrow adipose tissue at 7 T: Method comparison and in vivo feasibility

Resolving the omega‐3 methyl resonance with long echo time magnetic resonance spectroscopy in mouse adipose tissue at 9.4 T

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