Assessment of sperm quality through fluorometry and sperm chromatin structure assay in relation to field fertility of frozen-thawed semen from Swedish AI bulls

Januškauskas, Aloyzas; Johannisson, Anders; Rodriguez‐Martinez, Heriberto

doi:10.1016/s0093-691x(01)00456-3

Cited by 116 publications

(94 citation statements)

References 30 publications

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“…However, the correlation of this parameter with fertility is questionable. Some studies have shown positive correlation with field fertility (Januskauskas et al 2001) whereas others failed to show any correlation (Bailey et al 1994;Zhang et al 1999). In the present study, although the percentage of motile sperm before freezing was not correlated with nonreturn rate, it was correlated positively with increase in the percentage of acrosome reacted sperm at 4 h post-thaw and the rate of sperm-zona binding.…”

Section: Discussioncontrasting

confidence: 71%

Development of in vitro tests to predict fertility of bulls

Giritharan¹,

Ramakrishnappa²,

Balendran³

et al. 2005

Can. J. Anim. Sci.

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of in vitro tests to predict fertility of bulls. Can. J. Anim. Sci. 85: 47-52. The overall objective was to develop an in vitro test to predict fertility of bulls in the field. We investigated the bull effect on in vitro embryo production, zona binding and acrosome reaction, and the correlation of this effect to field fertility measured by 60-90 d non-return rate. Frozen semen from three separate ejaculates of eight unrelated young bulls, obtained from an artificial insemination (AI) center, was used. On thawing, ejaculates from each bull were pooled, motile sperm were selected and (a) subjected to immunofluorescent assay at 0 and 4 h of incubation in capacitation medium to assess acrosome status, (b) used in an in vitro fertilization assay system to assess cleavage and blastocyst production rates, and (c) sperm-zona binding assay was carried out to determine the number of sperm bound to the zona pellucida of mature oocytes. Percentage of pre-freeze motile sperm (PrFM) and non-return rate data were obtained from the AI center. PrFM, percentage of acrosome reacted sperm at 0 h (AR1), increase in percentage of acrosome reacted sperm after 4 h (InAR) and sperm-zona binding rates (ZB) differed (P < 0.05) among sperm samples obtained from different young bulls. Significant correlations (P < 0.05) were observed between PrFM and AR1 (r = -0.31), InAR (r = 0.36), and ZB (r = 0.32). AR1 was negatively correlated to ZB (r = -0.27) and cleavage rate (r = -0.20), InAR was positively correlated with ZB (r = 0.31) and cleavage rate (r = 0.26). None of the in vitro tests was correlated with non-return rate. These findings indicate that along with pre-freeze motility, a combination of in vitro tests including the percentage of spontaneously acrosome reacted sperm at thawing, might be useful in predicting bull field fertility. Such a combination of assays, however, has yet to be determined. (r = 0,26). Aucun test in vitro ne présente de corrélation avec le taux de non-retour. Ces constatations indiquent qu'outre la motilité des spermatozoïdes avant congélation, la combinaison de divers tests in vitro, dont le pourcentage de spermatozoïdes dont l'acrosome réagit spontanément à la décongélation pourrait avoir son utilité pour prédire la fécondité des taureaux sur le terrain. Reste à établir la combinaison de tests qui convient à un tel usage. Mots clés:Fécondité sur le terrain, réaction de l'acrosome, fixation à la zone pellucide, fécondation IV, évaluation de la féconditéArtificial insemination is one of the most successful reproductive technologies developed to improve reproductive efficiency of farm animals, especially dairy cattle. Accurate evaluation of the fertility of bulls used for AI purposes is of utmost importance since a single ejaculate provides several insemination doses and shows much influence on the reproductive potential of a herd (Rodriguez-Martinez and Larsson 1998). Therefore, the basic purpose of semen evaluation procedures is to ensure that only good quality and highly fertile semen is used for AI purposes....

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Section: Discussioncontrasting

confidence: 71%

Development of in vitro tests to predict fertility of bulls

Giritharan¹,

Ramakrishnappa²,

Balendran³

et al. 2005

Can. J. Anim. Sci.

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“…Combinations of several assays are needed to predict the fertility of a given semen sample (Graham et al, 1990;Rodriguez-Martinez, 2003). Januskauskas et al (2001) found a higher correlation between predicted value with known fertility when motility assessments were combined with other parameters of sperm function (r 2 =0.83) in AI bulls. However, several procedures have been used to evaluate the physiological and morphological characteristics thereby differentiating viable cells from dead cells, such as plasma membrane integrity (Mayer et al, 1951;Swanson and Bearden, 1951;Hackett and Macpherson, 1965) and acrosomal integrity (Wells and Awa, 1970;Chacarov and Mollova, 1976;Cross and Meizel, 1989;Ericsson, 1989;Axnér et al, 2002) but the number of cells examined by these two procedures are small.…”

Section: In Vitro Assessment Of Semen Fertilitymentioning

confidence: 93%

Induced immotility during long-term storage at +5°C does not prolong survival of dog spermatozoa

Shahiduzzaman

Linde‐Forsberg

2007

Theriogenology

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The days are not so far away when sex sorted preserved liquid semen will be available in drug stores saying "Let me stay cool, I will give you beautiful puppies".To my parents (Abdus Satter and Jamela Khatun ) Preservation of canine semen by extension and chilling has provided the AI veterinarians with an opportunity to overcome limitations of both time and space. Several parameters are assessed to predict the fertility of canine spermatozoa for a given sample. Motility is one of them. Spermatozoa stay immotile while preserved in CLONE (Cryogenetic Laboratories of New England, Inc, USA) Chilled Semen Extender whereas they maintain motility over the whole 23 days period of study while preserved in a Tris egg yolk glucose extender at 5°C.The aim of this study was to investigate whether the immotility induced by the CLONE chilled semen extender prolongs the lifespan of dog spermatozoa stored at 5°C, compared with a Tris-egg yolk-glucose (TG) extender, which maintains motility. Semen from eleven dogs was pooled, split in four aliquots, centrifuged, and the supernatants removed. The four sperm pellets were mixed with a TG extender; with the CLONE chilled semen (CL) extender; with TG extender mixed with TG but without egg yolk and with less glucose (TG+A TG ); or with the CLONE extender mixed with the CLONE activator (CL+A CL ) to a final sperm concentration of 200 x 10 6 spermatozoa / mL. The samples were stored at 5ºC for 23 days and examined twelve times for sperm motility by computer-assisted sperm analysis (CASA), as well as for plasma membrane and acrosome integrity, glucose consumption, and DNA fragmentation index (DFI). The experiment was performed in triplicate. The third replicate was examined for presence of bacteria. For the statistical calculations, the 23 experimental days were divided into three periods, the first period comprising days 1-8, the second period days 9-14, and the third period days 15-23. Total motility (TM%) did not differ between extenders in the first period, but was higher in TG and TG+A TG compared with CL+A CL in the second and third periods, and with CL in the third period (P<0.05). Progressive motility (PM%) was higher in TG (P<0.0001), TG+A TG (P=0.0007) and CL+A CL (P=0.0176) than in CL in the first period. Both TG and TG+A TG maintained higher PM% than did CL and CL+A CL in the second and third period. Tris-egg yolkglucose and TG+A TG maintained higher straight line velocity (VSL) and average path velocity (VAP) compared with CL and CL+A CL during the whole experimental period. Curvilinear velocity (VCL) was higher in TG, CL, and TG+A TG than in CL+A CL samples (P<0.05) in the first and third periods. No differences in lateral head displacement (LHD) were observed between extenders or over time. Acrosome and plasma membrane integrity decreased over time, but there were no differences among the extenders in the first and second periods (P>0.05). In the third period, however, TG, TG+A TG , and CL maintained acrosome and plasma membrane integrity better than CL+A CL . Glucose consu...

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“…Existe controvérsia entre estudos em humanos [72,73] e bovinos em relação aos testes que avaliam a estrutura da cromatina e sua associação com fertilidade, embora em humanos a técnica scsa tenha comprovada associação clínica com fertilidade, em bovinos os resultados não são conclusivos [74][75][76] já que cada teste avalia uma particularidade do dano ao dna, e o número de avaliações dos ensaios não permite associação estatística.…”

Section: Estudo Da Cromatina Espermáticaunclassified

Avaliações seminais e novas ferramentas genômicas na estimação da fertilidade em touros

Vega

Maciel

2015

Spei Domus

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Cómo citar este artículo: Ortiz-Vega WH, Maciel-Júnior VL, Quirino CR. Avaliações seminais e novas ferramentas genômicas na estimação da fertilidade em touros. Spei Domus. 2015;11(23) Resumo. Tema e alcance: a estimativa da fertilidade do reprodutor é uma ferramenta importante na escolha do macho, não só porque reflete o estado individual do reprodutor, mas também porque dito resultado influencia o futuro do rebanho. A presente revisão aborda o assunto fertilidade no macho bovino sob a perspectiva da biotecnologia do sêmen. Características: descreve as características seminais que influenciam o potencial reprodutivo e apresenta de forma geral técnicas laboratoriais rotineiras e inovadoras na avaliação de espermatozoides. Documentos base na área de biotecnologia de sêmen assim como artigos das últimas quatro décadas foram utilizados na revisão; além disso, aspectos-chave de manuais e livros de difusão mundial complementam o acervo bibliográfico referenciado. Achados: o amplo número de estudos sobre o tema e a diversidade nos resultados pressupõem vazios no conhecimento que abrem uma oportunidade na utilização de ferramentas genômicas e permitem explicar com maior acurácia os diferentes eventos fisiológicos presentes na expressão das características seminais referentes à fertilidade. Conclusões: a variância genética e as mudanças epigenéticas podem estar influenciando o potencial reprodutivo no macho; fenômenos funcionais ainda pouco conhecidos precisam ser elucidados no intuito de compreender e, talvez, alterar essa variação entre e inter reprodutores.Palavras-chave: espermatozoide, inseminação artificial, marcador molecular, proteômica.

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Assessment of sperm quality through fluorometry and sperm chromatin structure assay in relation to field fertility of frozen-thawed semen from Swedish AI bulls

Cited by 116 publications

References 30 publications

Development of in vitro tests to predict fertility of bulls

Development of in vitro tests to predict fertility of bulls

Induced immotility during long-term storage at +5°C does not prolong survival of dog spermatozoa

Avaliações seminais e novas ferramentas genômicas na estimação da fertilidade em touros

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