Assessment Of Sperm Quality Analyzer Ii B: Comparison With Manual Semen Analysis And Casa

Fuse, Hideki; Akashi, Takuya; Nozaki, Tomoyoshi; Nishio, Remon; Mizuno, Ichiro

doi:10.1080/014850190513012

Cited by 6 publications

(7 citation statements)

References 12 publications

(9 reference statements)

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“…Our study shows that sperm concentration results obtained by the SQA-V are in agreement with manual results, and our findings are similar to those reported earlier by Akashi et al (24) and Fuse et al (25). We found no significant linear deviation when quantifying the agreement between the two methods.…”

Section: Discussionsupporting

confidence: 94%

Automation is the key to standardized semen analysis using the automated SQA-V sperm quality analyzer

Agarwal

Sharma

2007

Fertility and Sterility

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Section: Discussionsupporting

confidence: 94%

Automation is the key to standardized semen analysis using the automated SQA-V sperm quality analyzer

Agarwal

Sharma

2007

Fertility and Sterility

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“…Therefore, sperm velocity, one of the CASA estimates, is a sensitive indicator of sperm functional ability in a postcoital test, artificial insemination with the husband's semen [7] and fertilization rates in IVF [8,18]. Up to now, we also have shown that CASA and SQA are likely to be useful in the clinical practice of infertility as a screening test for semen quality [9].…”

Section: Discussionmentioning

confidence: 99%

“…Fluctuations in OD are registered by a photometric cell and the frequency of the analog wave-like electrical signal is converted into a digital signal to provide a numerical output, and the various parameters are expressed. Same as SQA in former version [2,5,[9][10][11], since SQA-V recognizes only motile particles, problems with debris, which may have 440 T. Akashi et al influenced the difference of the sperm concentration between SQA-V and CASA, have been excluded. And poor conditions, including poor motion or low sperm concentration [2,19] also may influence the difference of the estimates between SQA-V and CASA.…”

Section: Discussionmentioning

confidence: 99%

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Usefulness of Sperm Quality Analyzer-v (Sqa-V) for the Assessment of Sperm Quality in Infertile Men

Akashi¹,

Mizuno²,

Okumura³

et al. 2005

Archives of Andrology

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& The aim of this study was to evaluate the correlation of new of Sperm Quality Analyzer (SQA-V) with the computer-assisted sperm analysis (CASA) and manual semen analysis estimates. One hundred five fresh semen samples were analyzed using SQA-V and CASA and manual semen analysis. Significant correlations of sperm concentration (p < 0.0001), sperm motility (p < 0.0001), and normal morphology (p < 0.0001) were observed between SQA-V variables and manual semen analysis estimates. There also were significant correlations of sperm concentration (p < 0.0001), sperm motility (p < 0.0001), and sperm velocity (p ¼ 0.0235) between SQA-V variables and CASA estimates. Meanwhile, it did not correlate with amplitude of lateral head displacement, beat cross frequency, lineality assessed by CASA. The value of the sperm concentration and the sperm motility measured by SQA-V showed high correlations with the value of those measured by CASA and manual semen analysis. In addition, velocity and sperm morphology may also be evaluated to some extent using SQA-V.

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“…These systems are pre-calibrated vs. manual analysis with strict conformity to WHO 5th Edition and follow regular quality control protocols [9]. Several studies have demonstrated that automated sperm analyzers could provide an accurate and highly correlated alternative to manual sperm analysis [9][10][11][12][13][14][15][16][17]. Basically, there are two categories of automated sperm analyzers on the market which can be characterized by their detection technology.…”

Section: Introductionmentioning

confidence: 99%

Double-blind prospective study comparing two automated sperm analyzers versus manual semen assessment

Lammers¹,

Splingart

Barrière

et al. 2013

J Assist Reprod Genet

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Purpose Despite controversy regarding its clinical value, male fertility investigation mainly relies on semen analysis. Even though reference guidelines are available, manual sperm analysis still suffers from analytical variability, thus questioning the interest of automated sperm analysis systems. The aim of this study is to compared automated computerized semen analysis systems (SQA-V GOLD and CASA CEROS) to the conventional manual method in terms of accuracy and precision. Methods We included 250 men in this double-blind prospective study. The SQA-V GOLD (Medical Electronic Systems) and CEROS, CASA system (Hamilton Thorne) were compared to the standard manual assessment based on the WHO 5th Edition. The main outcome measures were sperm concentration, total sperm number, total motility, progressive motility, non-progressive motility, morphology, motile sperm concentration (MSC) and progressively motile sperm concentration (PMSC) with the three methods. Results Statistical analysis of the test results from the automated systems and the manual method demonstrated no significant differences for most of the semen parameters. The Spearman coefficients of rank correlation (rho) for CASA and the SQA-V GOLD automated systems vs. the manual method were: Sperm concentration (0.95 and 0.95), total sperm number (0.95 and 0.95), MSC (0.94 and 0.96) and PMSC (0.94 and 0.93) correspondingly. Concerning sperm morphology, both automated systems demonstrated high specificity (Sp) and negative predictive values (NPV), despite significantly different medians (CASA: 83.7 % for Sp and 95.2 % for NPV, SQA-V: 97.9 % for Sp and 92.5 %). The highest precision (lowest 95 % confidence interval for duplicate tests) for all semen variables was found in the SQA-V GOLD. Conclusions The advantages of using automated semen analysers are: Standardization, speed (lower turnaround time), precision, reduced potential for human error, automated data recording and less need for highly skilled professionals to run the systems. The disadvantages of using automated systems are: notably the problem with testing some atypical samples and the inability to perform an assessment of morphology abnormalities. Based on the results of this study, the SQA-V Gold demonstrated better agreement vs. the manual method. In conclusion, automated semen analyzers can be used for routine semen analysis providing rapid clinically acceptable results with higher precision, and positively impacting laboratory standardization.

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Assessment Of Sperm Quality Analyzer Ii B: Comparison With Manual Semen Analysis And Casa

Cited by 6 publications

References 12 publications

Automation is the key to standardized semen analysis using the automated SQA-V sperm quality analyzer

Automation is the key to standardized semen analysis using the automated SQA-V sperm quality analyzer

Usefulness of Sperm Quality Analyzer-v (Sqa-V) for the Assessment of Sperm Quality in Infertile Men

Double-blind prospective study comparing two automated sperm analyzers versus manual semen assessment

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