Assessment of sperm DNA integrity within the Palaemon longirostris (H. ) population of the Seine estuary

Abstract

The interpretation of biomarkers in natura should be based on a referential of expected values in uncontaminated conditions. Nevertheless, to build a reference data set of biomarker responses in estuarine areas, which receive chronic pollution loads due to their transition position between continent and sea, is impossible. In this context, the aim of the present work was to propose the use of laboratory recovery period to define a baseline for the measurement of sperm DNA damage by Comet assay in the estuarine prawn Palaemon longirostris. For that, sperm DNA integrity was observed after both a passive (i.e. 20 days in a clean environment) and an active (i.e. forced renewal of spermatophores) recovery of wild P. longirostris specimens from the Seine estuary, in laboratory conditions. Then, the levels of sperm DNA damage recorded within the P. longirostris population of the Seine estuary, during six campaigns of sampling from April 2015 to October 2017, have been interpreted according to the defined threshold values. The results showed a persistence in the level of DNA damage after 20-day in clean environment with the passive recovery. This strategy was inconclusive to reach a baseline level but it revealed the lack of DNA repair mechanisms. For the active recovery, a decrease of 54 % of the level of DNA damage has been observed after the first renewal of spermatophores and this level stabilized after the second renewal. On the basis of this second strategy, we defined a mean basal value of sperm DNA damage of 54.9 A.U. and a maximum threshold of 69.7 A.U. (i.e. 95 %-CI). The analysis of the results using the reference value highlighted significant abnormal sperm DNA damage within the native population of P. longirostris from the Seine estuary on all stations during the six-sampling campaigns.

Coupling caging and proteomics on the European flounder (Platichthys flesus) to assess the estuarine water quality at micro scale

“…The Seine estuary is one of the most anthropogenically polluted estuaries in Europe (Carpentier et al, 2002;Cachot et al, 2006;Burgeot et al, 2017). Its basin covers more than a quarter of the French population and includes major harbours, industrial and agricultural areas (Mouny & Dauvin, 2002;Dévier et al, 2013;Erraud et al, 2018). Thus, the Seine estuary is subjected to eutrophication, organic and metallic contaminations (Blanchet et al, 2008;Dauvin, 2018).…”

“…The growth and survival of fish depend directly of prey availability and predation, but also of hydrological conditions and anthropogenic factors (Amara et al, 2007(Amara et al, , 2009. A large panel of pollutants (metals, PAHs, PCBs, …) are monitored in the Seine estuary, and numerous studies explored the resident wild life responses to xenobiotics in this highly polluted ecosystem (Ohe et al, 2004;Marchand et al, 2004;Galland et al, 2013; Journal Pre-proof J o u r n a l P r e -p r o o f 4 et Borcier et al, 2016;Erraud et al, 2018). In order to monitor the health status of fish in natura, many studies have measured biomarkers as cost-effective, sensitive and early warning signals of pollution in aquatic ecosystems (Amiard-Triquet and Berthet, 2015).…”

Influence of Storage Time on the DNA Integrity and Viability of Spermatozoa of the Spider Crab Maja brachydactyla

“…Thus, Lacaze et al [24] detected it from four days after ejaculation in Gammarus fossarum, including a 5% damage in the DNA within the tail of the comet from the control individuals [47]. Rani et al [48] found that in Macrobrachium rosenbergii, the percentage of genetic damage in sperm in the control individuals is around 20%, while Erraud et al [71,72] found very high values of genetic damage in the Palaemon longirostris prawns obtained on the Seine estuary (naturally exposed to environmental contamination). On the contrary, Feng et al [49] observed only little sperm fragmentation in the Penaeus monodon control individuals.…”

Association between semen quality among men with different occupational exposures and risk of recurrent spontaneous abortion in island residents

“…45 An assessment conducted on the Seine Estuary showed significant abnormal sperm DNA integrity within the native population of Palaemon longirostris, which may be caused by the accumulation of micro-pollutants such as polycyclic aromatic hydrocarbons (PAHs), trace metals and PCB. 46 More importantly, sperm DNA damages persist even 20 days after toxic stress cessation.…”