Assessment of spatial memory in mice

Sharma, Sunita; Rakoczy, Sharlene; Brown‐Borg, Holly M.

doi:10.1016/j.lfs.2010.09.004

Cited by 269 publications

(204 citation statements)

References 368 publications

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“…We observed no differences in motor coordination (Fig. S4A), but learning and memory, as judged by validated tests (18), were impaired in Cdh1 cKO mice ( Fig. S4 B and C).…”

Section: Resultsmentioning

confidence: 81%

APC/C ^Cdh1 -Rock2 pathway controls dendritic integrity and memory

Bobo-Jiménez

Delgado‐Esteban

Angibaud

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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Disruption of neuronal morphology contributes to the pathology of neurodegenerative disorders such as Alzheimer's disease (AD). However, the underlying molecular mechanisms are unknown. Here, we show that postnatal deletion of Cdh1, a cofactor of the anaphasepromoting complex/cyclosome (APC/C) ubiquitin ligase in neurons [Cdh1 conditional knockout (cKO)], disrupts dendrite arborization and causes dendritic spine and synapse loss in the cortex and hippocampus, concomitant with memory impairment and neurodegeneration, in adult mice. We found that the dendrite destabilizer Rho protein kinase 2 (Rock2), which accumulates in the brain of AD patients, is an APC/C Cdh1 substrate in vivo and that Rock2 protein and activity increased in the cortex and hippocampus of Cdh1 cKO mice. In these animals, inhibition of Rock activity, using the clinically approved drug fasudil, prevented dendritic network disorganization, memory loss, and neurodegeneration. Thus, APC/C Cdh1 -mediated degradation of Rock2 maintains the dendritic network, memory formation, and neuronal survival, suggesting that pharmacological inhibition of aberrantly accumulated Rock2 may be a suitable therapeutic strategy against neurodegeneration.APC/C Cdh1 | Rock | dendrite | memory | neurodegeneration

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“…We observed no differences in motor coordination (Fig. S4A), but learning and memory, as judged by validated tests (18), were impaired in Cdh1 cKO mice ( Fig. S4 B and C).…”

Section: Resultsmentioning

confidence: 81%

APC/C ^Cdh1 -Rock2 pathway controls dendritic integrity and memory

Bobo-Jiménez

Delgado‐Esteban

Angibaud

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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“…The Morris water maze test was used to examine the effect of induced mastication on spatial learning ability, as previously described (35). Although the group fed the hard diet showed a significant decrease in escape latency only at 10 days compared with the group fed the normal diet (Fig.…”

Section: Discussionmentioning

confidence: 99%

Forced mastication increases survival of adult neural stem cells in the hippocampal dentate gyrus

Akazawa

Kitamura

Fujihara

et al. 2012

International Journal of Molecular Medicine

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Abstract. In this study, we examined the effect of forced mastication on neurogenesis in the hippocampal dentate gyrus (DG) of adult mice. Six-week-old mice were subjected to either a hard or normal diet for 13 weeks. They received a daily injection of bromodeoxyuridine (BrdU) for 12 consecutive days beginning at 14 weeks of age. The number of BrdU-positive cells in the DG was counted 1 day after and 5 weeks after the final BrdU injection. The number of BrdU-positive cells 1 day after injection did not differ between the 2 diet groups. However, the number of BrdU-positive cells in the group fed the hard diet was significantly increased 5 weeks after BrdU injection compared to the group fed the normal diet. The results of the Morris water maze test showed that mice fed a hard diet required significantly less time to reach the platform than the control mice when tested at 10 days. Moreover, mice in the group fed the hard diet spent significantly more time in the former platform area than the group fed the normal diet, indicating that hard diet feeding improved spatial memory compared to normal diet feeding. Real-time PCR analysis showed that the expression of glutamate receptor 1 mRNA was significantly increased in the group fed the hard diet compared with the group fed the normal diet. These results suggest that mastication increases the survival of adult neural stem cells in the hippocampal DG.

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“…Then, spatial, recognition, and associative memories were analyzed ( Figure 4). Spatial memory was evaluated by the T-maze spontaneous alternation task (T-SAT) and the object location task (OLT) (40,41), both behavioral tasks sensitive to hippocampal dysfunction albeit not completely dependent (42)(43)(44). In the T-SAT test, mice have the natural tendency to alternate and enter the previously unvisited arm (novel arm) in a T-maze apparatus.…”

Section: Increased P75mentioning

confidence: 99%