Assessment of snake DNA barcodes based on mitochondrial COI and Cytb genes revealed multiple putative cryptic species in Thailand

Laopichienpong, Nararat; Muangmai, Narongrit; Supikamolseni, A; Twilprawat, Panupon; Chanhome, Lawan; Suntrarachun, Sunutcha; Peyachoknagul, Surin; Srikulnath, Kornsorn

doi:10.1016/j.gene.2016.09.017

Cited by 40 publications

(32 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Analysis of N. kaouthia based on meristic and linear measurements found no differentiation (Wüster et al, ), while “typical” and Suphan (white) phase cobras were found to be genetically identical, although only four samples of N. kaouthia were included. Other investigations of genetic differentiation in snake taxa have generally yielded inconclusive results such as Elapidae in Asia, Africa and America (Slowinski & Keogh, ), sequences of caenophidian snakes from GenBank (Kelly, Barker, & Villet, ; Vidal et al, ), sequences of Colubroidea snakes from GenBank (Lawson, Slowinski, Crother, & Burbrink, ; Yan, Li, & Zhou, ) and many snake species in Thailand (Laopichienpong et al, ).…”

Section: Introductionmentioning

confidence: 99%

Geographical differentiation and cryptic diversity in the monocled cobra, Naja kaouthia (Elapidae), from Thailand

et al. 2019

Self Cite

View full text Add to dashboard Cite

South‐East Asia has an exceptionally high diversity of snakes, with more than 250 snake species currently recorded from Thailand. This diversity likely reflects the diverse range of geographical and climatic conditions under which they live, but the evolutionary history and population genetics of many snake species in South‐East Asia have been little investigated in comparison with morphological studies. Here, we investigated genetic variation in the monocled cobra, Naja kaouthia, Lesson, 1831, across its distribution range in Thailand using mitochondrial DNA (cytochrome b, control region) for ~100 individuals and the nuclear DNA gene (C‐mos) for a small subset. Using population genetic and phylogenetic methods, we show high levels of genetic variation between regional populations of this non‐spitting cobra, including the north‐eastern, north‐central and southern regions, in addition to a population on Pha‐ngan Island, 150 km offshore from the southern peninsula. Moreover, inclusion of the north‐eastern population renders N. kaouthia paraphyletic in relation to other regional Naja species. The north‐eastern population is therefore probably specifically distinct. Given that these cobras are otherwise undifferentiated based on colour and general appearance to the “typical” cobra type of this region, they would represent a cryptic species. As has been shown in other animal groups from Thailand, it is likely that the geographical characteristics and/or tectonic alteration of these regions have facilitated high levels of population divergence of N. kaouthia in this region. Our study highlights the need for dense sampling of snake populations to reveal their systematics, plan conservation and facilitate anti‐snake venom development.

show abstract

Section: Introductionmentioning

confidence: 99%

Geographical differentiation and cryptic diversity in the monocled cobra, Naja kaouthia (Elapidae), from Thailand

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…The use of molecular tools not only assist in the rapid species identification but also assure their evolutionary trends, diversification, and other genomic features to discriminate the population (Burbrink and Lawson 2007;Castoe et al 2012). Due to the efficacy of mitochondrial Cytb gene for determining boundaries between snake species (Slowinski and Keogh 2000;Laopichienpong et al 2016), the present study aimed to generate the molecular data of two morphologically identified Elapidae species from Mizoram state and contributed to the global databases (B. fasciatus (ZSI_SHT1): GenBank Accession No. MN853157, BOLD Process ID SKBIO001-20 and O. hannah (ZSI_SHT17): GenBank Accession No.…”

Section: Resultsmentioning

confidence: 99%

Mitochondrial DNA discriminates distinct population of two deadly snakes (Reptilia: Elapidae) in Northeast India

Kundu

Lalremsanga

Tyagi

et al. 2020

Mitochondrial DNA Part B

View full text Add to dashboard Cite

The DNA data of Indian snakes are scanty in the global database, especially from the northeastern states. The present study generated the mitochondrial Cytochrome b gene information of two morphologically identified deadly elapid species from Mizoram. Both, the King Cobra (Ophiophagus hannah) and Banded Krait (Bungarus fasciatus) showed monophyletic clades in the BA topology and cohesively clustered with the database sequences generated from distant geographical locations. The studied O. hannah depicted 2.7-7.6% K2P genetic distances with the specimens collected from China, Vietnam, and Thailand. Further, the northeast Indian B. fasciatus revealed 3.3-4% K2P genetic distance from Chinese, Vietnamese, Thailand, Indonesian, and Australian specimens. The TCS network showed distinct haplotypes for both the species collected from northeast India. The genetic information of these venomous snakes would be helpful for further rapid identification from the museum as well as from roadkilled specimens, curbing the venom poaching and medical avenues.

show abstract

“…Apart from taxonomic studies, few integrated approaches have been induced to understand this charismatic reptile group (Gvo zd ık et al 2010(Gvo zd ık et al , 2013Pan et al 2015;Song et al 2015, Lavin andGirman 2019;Cai et al 2020). The advancement of molecular tools is not only helpful to identify species, but is valuable information to reconstruct phylogenetic relationship (Wiens and Slingluff 2001;Vidal and Hedges 2005;Townsend et al 2008;W€ uster et al 2008;Vidal and Hedges 2009;Wiens et al 2010;Mulcahy et al 2012;Pyron et al 2013), to detect cryptic diversity (Laopichienpong et al 2016), and to understand the origin as well as the group diversification (Sanmart ın et al 2001; Burbrink and Lawson 2007;Guo et al 2012). In previous studies, both nuclear and mitochondrial genes were successfully used to discriminate Anguinae species, including D. gracilis (Lavin and Girman 2019).…”

Section: Introductionmentioning

confidence: 99%

DNA barcoding reveals distinct population of Dopasia gracilis (Squamata: Anguidae) in Mizoram, Northeast India

Kundu

Lalremsanga

Biakzuala

et al. 2020

Mitochondrial DNA Part B

View full text Add to dashboard Cite

The DNA barcode data of Asian Glass Lizard, Dopasia gracilis, is limited in the global database, especially from India. The present study aimed to generate a barcode sequence of morphologically identified D. gracilis from the Mizoram state in northeast India and compared with other Anguidae species. The studied species showed monophyletic clustering in the Bayesian analysis (BA) phylogeny with strong posterior probability support and also discriminated sufficient Kimura 2 parameter genetic distances. The barcode data of D. gracilis revealed high intra-species genetic variability and formed two clusters in BA phylogeny. The Templeton, Crandall, and Sing network also depicted four different haplotypes within the barcode sequences of D. gracilis. The DNA sequences generated from northeast India showed 6.5-6.6% and 7.3% genetic distances with the sequences generated from Yunnan Province and Tibetan Plateau, respectively. Considering the high genetic distances, multiple clustering, and distinct haplotypes, the present study assumed the presence of possible cryptic diversity of D. gracilis in the Indochina sub-region and a distinct population in northeast India. We recommended the generation of more DNA information from different localities to elucidate the actual diversity of D. gracilis within the known range distribution.

show abstract

Assessment of snake DNA barcodes based on mitochondrial COI and Cytb genes revealed multiple putative cryptic species in Thailand

Cited by 40 publications

References 44 publications

Geographical differentiation and cryptic diversity in the monocled cobra, Naja kaouthia (Elapidae), from Thailand

Geographical differentiation and cryptic diversity in the monocled cobra, Naja kaouthia (Elapidae), from Thailand

Mitochondrial DNA discriminates distinct population of two deadly snakes (Reptilia: Elapidae) in Northeast India

DNA barcoding reveals distinct population of Dopasia gracilis (Squamata: Anguidae) in Mizoram, Northeast India

Contact Info

Product

Resources

About