Assessment of serum biomarkers in rats after exposure to pesticides of different chemical classes

Moser, Virginia C.; Stewart, Nicholas; Freeborn, Danielle L.; Crooks, James; MacMillan, Denise K.; Hedge, Joan M.; Wood, Charles E.; McMahen, Rebecca L.; Strynar, Mark J.; Herr, David W.

doi:10.1016/j.taap.2014.11.016

Cited by 39 publications

(34 citation statements)

References 42 publications

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“…This portion of the study was part of an investigation of the neurotoxic effects of fipronil in rodents (Freeborn et al, 2015; Moser et al, 2015). The animal facility is accredited by the American Association for Accreditation of Laboratory Animal Care International, and all protocols were approved by the National Health and Environmental Effects Research Laboratory Institutional Animal Care and Use Committee at the United States Environmental Protection Agency.…”

Section: Methodsmentioning

confidence: 99%

Identification of fipronil metabolites by time-of-flight mass spectrometry for application in a human exposure study

McMahen

Strynar

Dagnino

et al. 2015

Environment International

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Fipronil is a phenylpyrazole insecticide commonly used in residential and agricultural applications. To understand more about the potential risks for human exposure associated with fipronil, urine and serum from dosed Long Evans adult rats (5 and 10 mg/kg bw) were analyzed to identify metabolites as potential biomarkers for use in human biomonitoring studies. Urine from treated rats was found to contain seven unique metabolites, two of which had not been previously reported—M4 and M7 which were putatively identified as a nitroso compound and an imine, respectively. Fipronil sulfone was confirmed to be the primary metabolite in rat serum. The fipronil metabolites identified in the respective matrices were then evaluated in matched human urine (n = 84) and serum (n = 96) samples from volunteers with no known pesticide exposures. Although no fipronil or metabolites were detected in human urine, fipronil sulfone was present in the serum of approximately 25% of the individuals at concentrations ranging from 0.1 to 4 ng/mL. These results indicate that many fipronil metabolites are produced following exposures in rats and that fipronil sulfone is a useful biomarker in human serum. Furthermore, human exposure to fipronil may occur regularly and require more extensive characterization.

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Section: Methodsmentioning

confidence: 99%

Identification of fipronil metabolites by time-of-flight mass spectrometry for application in a human exposure study

McMahen

Strynar

Dagnino

et al. 2015

Environment International

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“…Liang et al () reported an increase in free amino acids in the serum of rats following long‐term (60 days) exposure to permethrin. A separate study comparing the response from multiple classes of pesticide chemicals found that there was no substantial change in amino acids in rats treated with a high dose of permethrin for 2 hours (Moser et al, ). In this study, the response of amino acids to permethrin exposure was varied, with some increasing and others decreasing (Figure and Table S1), indicating that there is some response to the pesticide exposure, but that it is not a one‐way effect.…”

Section: Discussionmentioning

confidence: 99%

Untargeted metabolomics of neuronal cell culture: A model system for the toxicity testing of insecticide chemical exposure

Hayton

Mullaney

Trengove

2017

J of Applied Toxicology

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Toxicity testing is essential for the protection of human health from exposure to toxic environmental chemicals. As traditional toxicity testing is carried out using animal models, mammalian cell culture models are becoming an increasingly attractive alternative to animal testing. Combining the use of mammalian cell culture models with screening-style molecular profiling technologies, such as metabolomics, can uncover previously unknown biochemical bases of toxicity. We have used a mass spectrometry-based untargeted metabolomics approach to characterize for the first time the changes in the metabolome of the B50 cell line, an immortalised rat neuronal cell line, following acute exposure to two known neurotoxic chemicals that are common environmental contaminants; the pyrethroid insecticide permethrin and the organophosphate insecticide malathion. B50 cells were exposed to either the dosing vehicle (methanol) or an acute dose of either permethrin or malathion for 6 and 24 hours. Intracellular metabolites were profiled by gas chromatography-mass spectrometry. Using principal components analysis, we selected the key metabolites whose abundance was altered by chemical exposure. By considering the major fold changes in abundance (>2.0 or <0.5 from control) across these metabolites, we were able to elucidate important cellular events associated with toxic exposure including disrupted energy metabolism and attempted protective mechanisms from excitotoxicity. Our findings illustrate the ability of mammalian cell culture metabolomics to detect finer metabolic effects of acute exposure to known toxic chemicals, and validate the need for further development of this process in the application of trace-level dose and chronic toxicity studies, and toxicity testing of unknown chemicals.

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“…Moser et al, 2015 identified potential biomarkers in rat serum exposed to a different chemical class of pesticides (permethrin, deltamethrin, imidacloprid, carbaryl, triadimefon, fipronil), Finding mayor differences in acylcarnitines, amino acids and glycerophospholipids (36). Olsvik et al, (2015) presented the effect of vitamin E on fatty acid accumulation and carbohydrates metabolism and how vitamin E supplementation can modify chlorpyrifos toxicity in salmon liver cells (37).…”

Section: Recent Articlesmentioning

confidence: 99%

Metabolómica y Pesticidas: Revisión sistemática de literatura usando teoría de grafos para el análisis de referencias

Zuluaga

Robledo

Osorio-Zuluaga

et al. 2016

nova

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The systematic literature review presented here is designed to illustrate the role of metabonomics and metabolomics in pesticide exposure studies. The search was conducted in Thomson Reuters Web of Science (ISI Web of Knowledge) database. The references and citations for each article were downloaded for analysis. Graph theory was used to determine relevant articles and distinct relationships between classic and current research in this field through its structural characteristics. The initial network included 4423 nodes and 4978 links, from which indegree, outdegree and betweenness indicators were extracted. After preprocessing the data, the network was reduced to 415 nodes and 974 links. From this network, 80 articles with the highest score between the three indicators were extracted for review. This methodology allowed for the identification of different perspectives of metabolomic and metabonomic pesticide studies that included the mode and mechanism of action, toxicological and biological monitoring, environmental metabolomics, metabolism, dose response and biomarkers and its role in pesticide exposure.Keywords: Metabonomics; metabolomics; pesticides. ResumenLa revisión sistemática de literatura presentada a continuación tiene como objetivo dar a conocer el rol que han tenido la metabolómica en el estudio de la exposición a plaguicidas. La búsqueda se llevó a cabo en la base de datos Thomson Reuters Web of Science (ISI Web of Knowledge). Posteriormente, se descargaron todos los registros producto de resultado de la búsqueda y cada citación dentro de cada artículo. Estas referencias fueron analizadas mediante la teoría de grafos con el fin de identificar los artículos más relevantes, los artículos clásicos y recientes y los que presentan mayor intermediación en el tema de investigación. La red de citaciones fue construida con inicialmente con 4423 nodos (artículos) y 4978 enlaces (citaciones) a los cuales se les determinó los indicadores de grado de entrada, grado de salida y centralidad en el grafo. Posteriormente, esta red de citaciones fue procesada eliminando los artículos desconectados, reduciendo la red a 415 nodos y 974 enlaces. De esta red ya procesada se extrajeron 80 artículos que presentaron mayores indicadores de grado y centralidad. Finalmente, esta metodología permitió la identificación de diferentes perspectivas de los estudios metabolómicos y metabonómicos en la exposición a plaguicidas que incluyen estudios de modo de acción, mecanismos de acción, monitoreo biológico y toxicológico, metabolómica ambiental, metabolismo, dosis respuesta e identificación de biomarcadores.Palabras clave: Metabolómica, metabonómica, pesticidas.

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Assessment of serum biomarkers in rats after exposure to pesticides of different chemical classes

Cited by 39 publications

References 42 publications

Identification of fipronil metabolites by time-of-flight mass spectrometry for application in a human exposure study

Identification of fipronil metabolites by time-of-flight mass spectrometry for application in a human exposure study

Untargeted metabolomics of neuronal cell culture: A model system for the toxicity testing of insecticide chemical exposure

Metabolómica y Pesticidas: Revisión sistemática de literatura usando teoría de grafos para el análisis de referencias

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